Subject: [ccp4bb] Do my SAXS data agree with the crystal structure?
Dear all,
I have solved a protein-DNA structure, and I also did SAXS to get
some ideas of the solution structure. The SAXS data were good, no
aggregation at all three tested concentrations. I tried to use Crysol
University of Pennsylvania
kgu...@mail.med.upenn.edu
215-573-7260 / 267-259-0082
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Xun
Lu
Sent: Saturday, June 16, 2012 2:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Do my SAXS
In theory, there should be a simple way to calculate P(r) directly from the
crystal structure rather than indirectly from the expected scattering curve.
Distribution of pair distances, r^2 weighted. This would remove any ambiguity
about choice of Dmax. ... but I can't think of any of the
: Saturday, June 16, 2012 2:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Do my SAXS data agree with the crystal structure?
Dear all,
I have solved a protein-DNA structure, and I also did SAXS to get
some ideas of the solution structure. The SAXS data were good
Hi Xun,
it is difficult to judge without seeing the P(r) plots, but seeing as
you have a dimer in your crystal structure and a dimer in your SAXS,
AND your Chi2 value seems reasonable for a good match between PX and
SAXS, I'd say you've got what you need.
A matching P(r) plot would be nice, but
-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Xun Lu
Sent: Saturday, June 16, 2012 2:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Do my SAXS data agree with the crystal structure?
Dear all,
I have solved a protein-DNA structure, and I also did SAXS