Re: [galaxy-user] FastQC in main galaxy server?

2012-03-07 Thread Jennifer Jackson
Hi Daniel, This was due to a small problem on a couple of our cluster nodes and we believe that this has been corrected. Please re-run you job and let us know if this error comes up again, Thank you for reporting the problem! Best, Jen Galaxy team On 3/6/12 11:49 AM, Daniel Sobral wrote:

Re: [galaxy-user] Metagenomics

2012-03-07 Thread Jennifer Jackson
Hi Vincent, Scott, Filtering raw hits is an important part of a metagenomics analysis pipeline. Please see the methods described in the published metagenomics analysis paper associated with this tool set: Koskovsky Pond S, Wadhawan S, Chiaromonte F, Ananda G, Chung W, Taylor J, and Nekrutenk

Re: [galaxy-user] description of "Find diagnostic hits"

2012-03-07 Thread William Hsiao
Hi Jen, Thank you for getting back to me and for pointing out the live projects, which definitely will provide some great resources for me. Cheers, Will On Mar 7, 2012, at 5:50 PM, Jennifer Jackson wrote: > Hi Will, > > An short description of what the Metagenomics tools do is on each of the

Re: [galaxy-user] description of "Find diagnostic hits"

2012-03-07 Thread Jennifer Jackson
Hi Will, An short description of what the Metagenomics tools do is on each of the tool forms. One bit that many be confusing is that what is often called the "Fetch Taxonomic Ranks" tool on these forms has been renamed to be the "Fetch taxonomic representation" tool. In short, the "Find diag

Re: [galaxy-user] question from a new Galaxy user

2012-03-07 Thread Jennifer Jackson
Hello Elwood, Just to double check (although you have probably already seen it), when using the FTP client, the same basic steps from this wiki were followed? Also please that you can FTP compressed data: http://wiki.g2.bx.psu.edu/FTPUpload Moving data from the FTP transfer area on the "Get

[galaxy-user] question from new Galaxy user

2012-03-07 Thread Elwood Linney
Hello: I apologize for bothering you but I am just beginning to work with mRNAseq data and it appears as though Galaxy will be quite useful but I suspect, even after looking some of the tutorials that I will be either making mistakes or just not have a good sense of things. I used "Fetch" off a ne

Re: [galaxy-user] Question about plotting circos plot

2012-03-07 Thread Björn Grüning
Hi Shamsher. We have a small initial wrapper for circos. Its not complete yet and we are not sure if its even possible to wrap such a complex tool in a good galaxy UI. But if you are interested i can share our code. Cheers, Bjoern > I wonder if is it possible to visualize mutation data in circul

[galaxy-user] Fwd: Question about plotting circos plot

2012-03-07 Thread shamsher jagat
My apology for re-posting the same question however I believe my first message was returned. I wonder if is it possible to visualize mutation data in circular plot termed as circos plot e.g @http://www.eurekalert.org/multimedia/pub/31019.php?from=181881 Any suggestion for an alternative tool wi

Re: [galaxy-user] Fwd: visualisation of a bam file

2012-03-07 Thread Jennifer Jackson
Visualization dataset index correction, please see below: On 3/7/12 6:56 AM, Jennifer Jackson wrote: Hello, I was able to create a visualization using hg19 as the reference and load this dataset and visualize it. It is important to allow the BAM file to index fully, do not navigate away from t

[galaxy-user] Dataset name in dataset file

2012-03-07 Thread Cittaro Davide
Hi all, I have a question about a weird usage of galaxy: suppose I have 23 files, each for a chromosome, suppose the dataset name (in history) contains the chromosome name but the dataset content don't (i.e. I only have chrom position and 2 associated values), is there a way to add a column so

Re: [galaxy-user] Fwd: visualisation of a bam file

2012-03-07 Thread Jennifer Jackson
Hello, I was able to create a visualization using hg19 as the reference and load this dataset and visualize it. It is important to allow the BAM file to index fully, do not navigate away from the page while this is going on. As a test, I also used SAMTools BAM-to-SAM, then SAM-to-BAM (with hg

[galaxy-user] bed to gff

2012-03-07 Thread Robin Mjelle
Dear Galaxy I have a problem converting Interval to GFF. I used the tool BED-to-GFFconverter. My Interval file contains 18 coulmns: *chr1 3330899833309020+ cel-let-7-5p0 chr1 33308999255 22M

[galaxy-user] Job running speed is really slow

2012-03-07 Thread 杨继文
Dear Galaxy team, I found glalaxy (website) is running really slow today. I just did some text manipulations over a very small file. It takes a long time till the gray colour (waiting) turns into yellow (running). Now the jobs are still waiting to be processed (gray colour). I have to analyz

[galaxy-user] Tophat

2012-03-07 Thread 杨继文
Dear all, This might be a silly question, but I couldn't figure it out by myself :-((. Could you please tell me how I can find out how many reads have been mapped to the genome after running Tophat for pair end RNA seq data? Thanks in advance. JIwen_