Hi César
we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for
cryosections of kidney biopsies (indirect immunofluorescence), it won't work
on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d
(BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buf
Dear People from Histonet
Could you share your experience with C4d Antibody ?
I couldn't get any results with the mouse monoclonal from Quidel.
I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe.
I work in a Public Hospital with many kidney transplantations per
For Sale
2 set of paraffin block drawers $25
20 or better cardboard slide holders (holds 20 slides) $1 each
Cathy Mayton
Wasatch Histo Consultants, Inc.
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Laurie, et al. -
This is what we do in my lab...
We use a computer quality control program called QPulse. Everytime an SOP
is changed (even slightly, such as vendor or incubation time change), the
new revision is made through the QPulse Document Draft program, and in the
process of making th
I am a firm believer in lab notebooks.
In it we record all routine maintenance, quality control, solution
preparation (especially dye lot numbers, source etc) as well as
variations to technique caused by vagaries of dye reagents.
Any developmental work is also recorded, trial of new dyes, techniq
Thanks for the tip! I was sure thi= s list would have the answers. I
actually didn't know about the archives un= til you mentioned it, and
did a search for it just now. Thanks so much!
Jenee S. Odani, D.V.M., Dip= l. ACVP
Veterinary Medical Officer
Hawaii State Veterinary = L
We are also a research lab and do both decal and non-decal bone from mouse to
horse. We have three Leicas, 2 RM 2255 and one older RM2155. They are all
motorized and we love them with tungsten carbide or disposable knives. We have
them for 3 and 4 years with never a problem.
Pam Marcum
What is the best microtome on the market these days?
We are a research lab sectioning mainly mouse tissue, and mainly
decalcified bone. We have beginners to experts in the lab all who
will need to use it.
It's been a while since I have been in the market for a microtome, so
although I have m
Laurie,
I have a binder that is for Continuous Quality Improvement. If something comes
up that is not part of my regular schedule of Quality Control or Quality
Improvement it goes in that binder. I have a form that I use to state the
reason for the Quality Improvement (what initiated the chang
I have running a experiment in rabbit that included the bone reconstruction
using mesenchymal stem cell seeded previously in calcium phosphate and calcium
silicate scaffold. I need to know if, after some time, the scaffold remains in
the tissue or they are reabsorbed and the bone is reconstru
I'm hoping someone can help me. We are looking for a copy of the Operations
Manual for a lift made by Lipshaw, Model 15BS. We use this for moving bodies
in our morgue, and can not find information about servicing it.
Thanks,
Toni
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Dear Histonetters,
Is there anyone out there that follows the JCAHO - Universal
Protocol/Time Out procedure when the pathologist perform his/her own
FNA's? We have a pathologist that will perform FNA's when asked and I
was told that when he performs these procedures that we need to be
following t
Anita, I have seen this when using plastic embedding molds. The molds
themselves hold an electric charge. It's annoying when your samples all migrate
to the edges.
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City,
I'm wondering if anyone has any helpful tips for deplastisizing Spurr.
We currently use saturated Sodium Hydroxide in 100% alcohol. We tried a
5% and a 1% solution as well, but still not perfect. The main issue we
are having is the tissue is lifting off of the slide during staining.
For microtomy
wondering if anyone else has had this problem? when embedding small core bxs
they are like they are charged and go off of the forceps to the edge of the
embedding mold. its like they are running away from the forceps. what would
be causing this? thanks so much.
anita dudley
providence hosp
I think that distilled water starts out at a ph of around 6-6.5, but usually
DI water is closer to 7. Over time, both will drop in pH unless you buy
specialized housing for the DI water. Since many people doing IHC at my
university are using RO water, which has a pH similar to what the pH of the
I think that distilled water starts out at a ph of around 6-6.5, but usually
DI water is closer to 7. Over time, both will drop in pH unless you buy
specialized housing for the DI water. Since many people doing IHC at my
university are using RO water, which has a pH similar to what the pH of the
I think that distilled water starts out at a ph of around 6-6.5, but usually
DI water is closer to 7. Over time, both will drop in pH unless you buy
specialized housing for the DI water. Since many people doing IHC at my
university are using RO water, which has a pH similar to what the pH of the
Dear Histonetters!
I was wondering if anyone is working with Desmoplasia markers? I am working
with xenograft models (human tumors implanted in mice). So the result is
human tumor cells and Murine stroma. I am trying to test various desmoplasia
markers found in literature, but almost all of them ar
I would like to add to the cooments of others concerning distilled water and
deionised water:
1) I think most histology labs use deionised water as it is cheaper and easier?
to obtain.
2) However, even deionised water will turn red with Schiff reagent.
3) For silver stain solutions, our lab uses
I would strongly recommend di Fiore's Atlas of Histology by Victor P.
Eroschenko. I don't know what edition its in now. I used it when I went through
my program. It is great for microscopic anatomy, especially when combined with
actual slide viewing.
Claire
Fro
So what does an Anne Preece go for? Can I retire too? I've got one of those
Sheehan's too.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From: histonet-b
I'm curious as to what others do, if anything, to document corrective
actions or improvements for H&E staining and/or tissue processing. For
example, I made changes to my biopsy processing program and to my breast
processing program which helped improve the cutting quality of both
types of tissue.
Make sure you get enough to have visitors!
Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lbla...@digestivespecialists.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Gosh - my retirement fund just appeared... a Sheehan for $2500? Wow! I
have one here (2nd edition) but not mine to sell - besides, I use it. I
do have a 3rd edition Lillie "Histopathologic Technic and Practical
Histochemistry" (1965), pre-autographed by me when I bought it that I'd
sell for the
Hi Jenee: You need to check the histonet archieves. For the last two weeks
there has been an on going conversation in regard to disposal of microscopic
slides. Since I am in a COR lab most of my slides are sent to the PI's that
order the slides therefore I do not have to deal with this as of
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