[Histonet] Re: Muscle biopsies

Toni Rathborne, Pathology Supervisor, Somerset Medical Center, Somerville NJ asks: Can anyone recommend a reputable lab (preferable upper-east coast) to send muscle biopsies to? The preparation of muscle biopsy specimens is very exacting - they need to be transported on wet ice, quite unfixed -

RE: [Histonet] Cutting paraffin sections in a warm room

a thermostat at all, and the microtome is inside the lab. I was wondering if anyone had any ideas on how to section in a warm room. Thanks, Adam __ Information from ESET Smart Security, version of virus signature database 4576 (20091105) __ The message was checked by ESET Smart

[Histonet] workflow

We currently have 3 histotechs at our lab. They arrive at 5 am, 7 am, and 8:30 am. The first tech embeds ½ of the workload and is supposed to cut ½ of the workload. They typically complete a couple of cases or 1 tray by 8 am. The second tech also embeds ½ of the work and cuts ½. The third

[Histonet] Processing small gi biopsies, skin lesions, currettings

I am in the process of reviewing our procedures in the gross room. Currently we have a few techs that do these small biopsies. Each one actually has a bachelor of science degree along with their HT certification. The number of these folks is shrinking and I want to include some other

[Histonet] Light Box to quench autofluorescence

Hello, I've received information from several sources saying that using a light box is a good way to quench autofluorescence for IF. Does anyone know where I can purchase one of these? Thanks ___ Histonet mailing list

Re: [Histonet] lab spill clean-up

I've always been in institutions (hey, no jokes) where more than a liter of spilled formalin, alcohol, xylene, etc is cleaned up by the in house haz mat people. From: kristen arvidson arvidsonkris...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Date: 11/05/2009 02:22 PM Subject:

[Histonet] lab spill clean-up

I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up.  Are there set guidelines for when you should call for help? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Processing small gi biopsies, skin lesions, currettings

Thanks for throwing this one out there. I am also interested in this topic. Can you please post replies to the net? Thanks !!! :-) --- On Thu, 11/5/09, Vickroy, Jim vickroy@mhsil.com wrote: From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] Processing small gi biopsies, skin

[Histonet] Looking for Ray Ortiz

Can someone tell Ray to contact me? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] OSCAR antibody

What do the letters that make up the name OSCAR mean ? There is a debate in my lab over it? :) Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 Notice from St. Joseph Health System:

[Histonet] Whole mount porcine trachea staining

Would anyone have an idea about how to stain whole porcine trachea so that the cartilage rings show prominently? There have been several excellent articles that use Alcian Blue or an Alcian Blue/Alizarin Red S combination to stain rodent tracheas (mouse/rat) and the pictures are wonderful

Re: [Histonet] OSCAR antibody

Patti Loykasek responded to that question a few weeks back: Actually the name stands for Our Second Cytokeratin Antibody Rocks! A bit of path humor (or at least Dr. Gown tried to be humorous!). We are truly a bit silly sometimes. Kelly Turner, HTL(ASCP)QIHC PhenoPath Laboratories Seattle, WA

[Histonet] cryopreservation of rodent nerves for frozen sections

Hello everyone, Happy Thursday. I am working on a project where the investigator is doing immunostaining on thoracic cranial nerves in rat on frozen sections. The initial sections were too riddled with ice artifact to be useable so we are trying again and I am doing sucrose cryopreservation

[Histonet] marilyn weiss will be out of the office as of 6 a.m.11/5/09 returningMonday 11/9

I will be out of the office starting 11/05/2009 and will not return until 11/09/2009. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. ___ Histonet mailing list

Re: [Histonet] lab spill clean-up

Our institution had an after hours spill of xylene (approx. 1/2 gallon). People panicked, and, long story short, called Hazmat. Of course, it was SuperBowl weekend, and it took them 3 hrs to go the distance of 1 hr. Our lab, clinical lab and emergency was closed down awaiting the clean-up

Re: [Histonet] Cutting paraffin sections in a warm room

One of the little things I learned along the way regarding bone sectioning: if time allows, trim (face) the blocks and leave them in the deep freeze (-?20) overnight. T Then when you are ready to section, take ONE block out at a time and place on ice. this trick is especially useful for us as we