Re: [Histonet] Sakura glas2

2018-07-18 Thread Mac Donald, Jennifer via Histonet
We use Permount and an aliphatic hydrocarbon clearing reagent. Jennifer Get Outlook for iOS From: Bitting, Angela K. via Histonet Sent: Wednesday, July 18, 2018 8:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura glas2

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2018-07-18 Thread Cristi Rigazio via Histonet
Have you checked the processors? It is easy to “mix up” the reagents. Has tissue collection changed? Are the specimens left out longer or placed on paper? Do you use sponges? Any chance the fixation time has been decreased? Sent from my iPhone > On Jul 18, 2018, at 3:46 PM, Mac Donald, Jen

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2018-07-18 Thread Mac Donald, Jennifer via Histonet
Is anyone using freezing spray? We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing “freezer burn” to the GI biopsies. Jennifer Get Outlook for iOS From: Char

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2018-07-18 Thread Charles Riley via Histonet
Our pathologists have said that we have had a few cases the past few days that are unreadable. They say the cells appear "cooked". Nothing in our process has changed, we have been using the same lot of reagents since the middle of June, and no errors were reported in our processing runs. Do anyon

Re: [Histonet] New Microtome

2018-07-18 Thread Terri Braud via Histonet
We are using a Leica 2235 and love it. Manual or Auto mode, the control panel is on a moveable pad, so can be used anywhere. It has a foot pedal if you like that too! The techs took to it like a duck to water and it cuts like butter! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Labo

Re: [Histonet] Microtomes

2018-07-18 Thread Laurie Colbert via Histonet
We have three Thermo 355S's.  They are nice because you can use them in the automated mode or manual mode.  All of our HT's use the manual mode, so now we just buy the manual version. Laurie Redmond HT (ASCP) Path MD -Original Message- From: Piche, Jessica via Histonet To: histonet S

[Histonet] Microtomes

2018-07-18 Thread Piche, Jessica via Histonet
Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control p