We first use a pH strip to test neutrality and then use a test kit to test for
formaldehyde. We get this from Fisher and the number is HC426043.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[ma
We have one too. We have been getting ribbons from Surgipath/Leica. The number
is 38V0590001F. we have Tech One Biomedical service it. They have been great.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwes
We use acetone to denature fatty tumors without any problems. They sit in it
for at least half an hour. I don't rinse them.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun..
We have had a Sakura tape coverslipper for 17 years with hardly any problems.
The slide storage quality is excellent.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@list
We have a Sakura tissue processor that is more than 25 years old and still
working very well with annual preventative maintenance.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-
It doesn't seem cold but in relation to how hot the other areas are it seems to
be sufficient. It has been very reliable. We have had three in the 22 years I
have used them and two are still in use.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message---
Same here.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Tuesday, January 14, 2014 9:53 AM
To: Jennifer MacDon
I have two chromo map kits for the Discovery I can send you.
Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine
Simonson
Sent
If anyone out there is using a decloaker for antigen retrieval, when doing ihc
staining, would you please contact me on my e-mail address? I have been
struggling with a BVD test for months. It is not working consistently and I
want to explore using this method along with our Benchmark XT stainer
That's what we do.
Lynn Burton
Animal Disease Lab
Galesburg, Il
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Tuesday, May 28, 2013 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histone
I have been very pleased with the Discovery and Benchmark from Ventana.
Lynn Burton
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal
Sent: Friday, May 17, 2013 2:59 PM
To: Nails, Felton
Cc: h
We have had a Sakura film coverslipper since 1997. It has had three service
calls, mostly in last 5 years. Those service calls were minor problems quickly
fixed. When we demoed several different coverslippers, we found this one to
have the least amount of "tweeking" involved. It has been a wonde
Both animal disease labs in the state of Illinois have used the Sakura machines
for the twenty years I have been here with great success. We also have aSakura
processor that has been going for 25 years and a coverslipper that has only had
3 service calls for minor problems in the past 15+ years.
Amen. I never voted for him
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Kemler
Sent: Monday, November 19, 2012 8:49 PM
To: Fellow HistoNetters
Subject: Re: [Histonet] 88305TC starting to hit the
We just got a Discovery XT and have had the Benchmark XT. It still works well
after 10 years.
Lynn
-Original Message-
From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org]
Sent: Monday, October 29, 2012 9:38 AM
To: Terri Brown; Sebree Linda A; Burton, Lynn; Gloria Tharp;
histonet
We have been dealing with Ventana for at least 10 years with very good service
and reliability. Their phone tech support is excellent and we have never waited
long for in house service.
Lynn Burton
Lab Associate
Animal Disease Lab
Galesburg, Il
-Original Message-
From: histonet-boun...@l
Perhaps they have a contact person since they do so much work throughout the US.
From: jsjurc...@comcast.net [mailto:jsjurc...@comcast.net]
Sent: Friday, September 21, 2012 3:16 PM
To: Jay Lundgren
Cc: Histonet; Burton, Lynn
Subject: Re: [Histonet] microtome
As I said, I'm not sure they
I would try Tech One Biomedical. They have a great staff and they have done all
of our repairs for a long time.
1-866-497-3033 www.techoneweb.com
Lynn Burton
Galesburg Animal Disease Lab
Galesburg, Il
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-bo
We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows:
780mL 95 %ETOH
100mL Eosin
10mL Phloxine B
4mL glacial acetic acid
We seem to be consistent. We have used this for 17 years.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
I have an operator guide.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
[allison_sc...@hchd.tmc.edu]
Sent: Tu
We have. I don't recall the amount dispensed but my notes say the cost, per
slide, is as follows:
LCS $.02
CC1.28
EZ Prep $.15
Reaction Buffer $.02
Ultra View kit $5.09
My brain keeps telling me the dispensed amount is 1,uL? Tech support at Ventana
is great about answering those questions.
Let's move on please.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine
(CDC/OID/NCEZID) [j...@cdc.gov]
Sent: We
The ultra view platform changed its clearing process in November. After
staining slides are to be run through two changes of soapy water and then
distilled water. After that is done they go on a slide dryer/warmer for at
least 15 minutes before going into xylene and then coverslipped. Our sales
One great thing is that it does everything on the machine. It deparrifinizes as
well as stains. The ultra view platform has even simplified the steps from
machine to coverslipping. It can be set to run overnight. It has delayed timers
for starting or ending.
Lynn Burton
Lab Assoc I
Animal Disea
I don't have any ideas but I am sorry to hear you are leaving. Good Luck with
your future.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On
We have used this product for many years as well. It has performed well without
the hazards of xylene. We still use xylene on the processor for cleaning. I
have not used it in special stains, only on the processor.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
I still use that. It is 70% with 5mL of hydrochloric acid if making 1L.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysa
Sorry Sara,
We make our own here too. I can't remember why exactly. One reason is the space
for a 50 gallon drum I believe. Good Luck. Bureaucrats always think personnel
is cheap enough to be expendable.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
___
Try Tech One Biomedical at www.techoneweb.com or 1-866-497-3033. They do good
work.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf
We have a 15 year old Sakura film coverslipper that has performed famously with
Venatana labels.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.ed
We calculated that to be right around $4.05 per slide. That does not include
labor.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf
Thanks for all the great info Pearl! I forget some of those easy things day to
day.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf
That could also be tendonitis related to the "snuff" joint. It starts with a D.
Dequarveins or something, someone will know. I got it when I was pregnant with
my second child. There are exercises for strengthening that tendon.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
Tech One Biomedical offers services in that area. 1-866-497-3033 techone
b...@attbi.com. We have used them for years with great success.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[
Fisher has a Hema 3 System in 500mL bottles for $144.75 that I use.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Po
]
Sent: Wednesday, January 05, 2011 1:32 PM
To: Burton, Lynn; sgoe...@mirnarx.com; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Stupid, stupid static!!
If you use lotion in the lab, won't it get on your slides and cause havoc
(big oil droplets)?
Jan Shivers
- Original Me
Use fabric softener sheets. You can wipe the blades and knife holders with
them. My daughter's American Girl magazine says to pour lotion in your hand,
rub it between your hands and while it is still shiny run your hands through
your hair to control static.
Lynn Burton
Lab Assoc I
Animal Disease
I am in need of references for stain protocols. The stain I am searching for in
particular is the modified Gimenez, also known as Pierce-Vanderkamp for
chlamydia and rickettsia. If anyone can point me in the right direction I would
appreciate it.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Ga
It has been my experience that if I use charged slides I get overstaining.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Chakib Boussahmain
Sent: Tue 8/31/2010 9:22 AM
To: histonet@li
From: Burton, Lynn
Sent: Tue 7/13/2010 2:17 PM
To: histonet-boun...@lists.utsouthwestern.edu
Subject: M. Bovis IHC question
If anyone out there is using M. Bovis antobody from Chemicon can you please
tell me what dilution you arer using? We had a computer
I am looking for a vendor of BVDV antibody for IHC testing. We had used
Syracuse Bioanalytical in the past. They seem to be gone. I would appreciate
any suggestions.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
___
Histonet mai
Where did you purchase them?
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Merriam
Sent: Thu 6/10/2010 7:57 AM
To: lau...@conxis.com
Cc: Histonet
Subject: Re: [Histonet] Histodeck
Does anyone out there have an IHC protocol, for Vimentin, in animal tissues,
that has been working for them on the Ventana Benchmark XT? I would appreciate
any suggestions at this point.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
___
Try Tech One Biomedical. I am not sure what you are asking for.
www.techoneweb.com
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Lucy Zong
Sent: Thu 10/22/2009 1:43 PM
To: histonet@l
Our dilemma has been that we aren't sure how they should be disposed of. We
have chemicals that are probably more than 30 years old.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kel
I read our SOP's on storage and referred to Dr. webb, our pathologist. His
feeling was that they, AAVLD, leave that up to each lab depending on the state
statutes regarding law suits. Our SOP says seven years on blocks and nine years
on slides. I would agree with you that as long as you have blo
I don't know what the COST to produce an H&E is but we charge outside entities
$8 each to stain them.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amy Johnson
Sent: Wed 9/2/2009 11:
We can do that but what kind of volume are you looking at? Where are you
located?
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
309-344-2451
From: histonet-boun...@lists.utsouthwestern.edu on behalf of b427...@aol.com
Sent: Thu 7/23/2009 7:02
The prices I have are on the list you sent. I couldn't find the CC2 price
quickly.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tiana Fountain
Sent: Fri 6/19/2009 10:42 AM
To: histo
Is your formalin picking up water from the air in the room? Check the humidity
level in your room.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Christine I.
Braaten
Sent: Thu 5/21/
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: Burton, Lynn
Sent: Tue 3/24/2009 10:31 AM
To: histonet-bou...@lists.utsouthwestern.edu
Subject: formalin neutralizers
What is everyone using to neutral their formalin? Who has the best
Dako did acquire Surgipath recently. The Surgipath rep said it has been a good
"marriage" for them so far.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pamela Marcum
Sent: Thu 3/12/
We have had some trouble but they are planning to replace them. We received a
letter that they had changed vendors for parts and found they need to switch
back. They did replace one section earlier when it stopped working completely.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 614
In the summertime ours is at around 60-70%, which seems to work well. In the
winter time it is less than 0 and I run a humidifier full out to try to achieve
20%. It is definitely easier in the summer.
Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401
_
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