Hello Histonet,
Wondering what those of you that do IHC on bones do to ensure that the tissue
remains on the slide during processing?
Thanks,
Sara McCabe HT(ASCP)
DuBois Regional Medical Center
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Hello Histonetters,
Wondering if anyone is using PMS2 Mismatch Repair Enzyme from Novocastra/Leica.
Have tried varied dilutions and retrievals without any luck. Any suggestions
would be great! Thanks!
Sara McCabe, BS, HT (ASCP)
DuBois Regional Medical Center
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Happy Friday Histonetters!
Anyone performing CMV by IHC? If so what are you using for a protocol? We
ordered CMV from Cell Marque but they give no suggestions for a protocol. We
are using the Leica Bond for our IHCs. Any input is greatly appreciated!
Thanks!
Sara McCabe, HT (ASCP)
DuBois Reg
Hi Nancy,
We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is
working great for us!
Sara McCabe, HT (ASCP)
DuBois Regional Medical Center
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We always put our patient and control tissue on the same slide. We have many
controls already cut and then we place the patient tissue below. If we are
using blanks that were previously cut then we cut the control and place that
below the patient tissue. We chose to not invest in the pre-marke