when. I'd like
to avoid traumatically amputating my arms if possible, thank you.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Geoff
Sent: Mon 9/17/2012 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: mouse test
m
Subject: [Histonet] RE: mouse testis in Bouins
Hi Margaret,
Our protocol for whole mouse embryos E14.5-E18.5 was to fix in
Bouin's for 5-7
days at room temp (I have gone longer, but it isn't exactly
recommended). Most
of the length of time, however, was to compensate for the large
nk
To: E. Wayne Johnson ; Jackie O'Connor
Cc: histonet
Sent: Sat, Sep 15, 2012 7:53 am
Subject: Re: [Histonet] RE: mouse testis in Bouins
>From CDC - not quite a lab, but in Jan. 2002, this company was "melting"
down the plastic from around capacitors, to regain the metals ins
believe most labs are getting away
from Bouins.
Jackie O'
-Original Message-
From: Frances Elizabeth Barron
To: histonet
Sent: Fri, Sep 14, 2012 12:21 pm
Subject: [Histonet] RE: mouse testis in Bouins
Hi Margaret,
Our protocol for whole mouse embryos E14.5-E18.5 was to fix in Bo
e most labs are getting away from Bouins.
Jackie O'
-Original Message-
From: Frances Elizabeth Barron
To: histonet
Sent: Fri, Sep 14, 2012 12:21 pm
Subject: [Histonet] RE: mouse testis in Bouins
Hi Margaret,
Our protocol for whole mouse embryos E14.5-E18.5 was to fix in Bouin's
o, and our testicle experts
are happy. I believe most labs are getting away from Bouins.
Jackie O'
-Original Message-
From: Frances Elizabeth Barron
To: histonet
Sent: Fri, Sep 14, 2012 12:21 pm
Subject: [Histonet] RE: mouse testis in Bouins
Hi Margaret,
Our protocol for whole mous
Hi Margaret,
Our protocol for whole mouse embryos E14.5-E18.5 was to fix in Bouin's for 5-7
days at room temp (I have gone longer, but it isn't exactly recommended). Most
of the length of time, however, was to compensate for the large tissue size and
need for good penetration. I'm not sure how
Haven't seen mouse balls in years. My laptop has a trackpad.
But seriously, folks, I concur - excessive time in Bouin's fixative
will harden the tissue - move it to 70% alcohol.
I've used Davidson's fixative (3 parts water, 3 parts alcohol, 2 parts
37% formaldehyde, 1 part acetic acid) with human
