[Histonet] brain sections falling off slides during IHC

2009-02-11 Thread Perry, Margaret
A few years ago we also had this problem. The brains were terrible. We found that changing our processing chemicals, especially the wax, more frequently got rid of most of our problems. We use Fisher plus slides. There is a difference in plus slides. I recommend you contact several distribut

Subject: [Histonet] brain sections falling off slides during IHC

2009-02-11 Thread Bull, Jennifer L.
t; lists.utsouthwestern.edu<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>] On Behalf Of Thurby, Christina Sent: Wednesday, February 11, 2009 7:38 AM To: histonet <@t> lists.utsouthwestern.edu<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Subject

Re: [Histonet] brain sections falling off slides during IHC

2009-02-11 Thread TF
stern.edu 抄送: 主题: [Histonet] brain sections falling off slides during IHC Hello all, Can any of you all with experience immunostaining brain sections please help. My sections are almost completely washing off the slides. I am using 10% NBF fixed canine brain sections. Embedded in paraffin

[Histonet] brain sections falling off slides during IHC

2009-02-11 Thread Thurby, Christina
Hello all, Can any of you all with experience immunostaining brain sections please help. My sections are almost completely washing off the slides. I am using 10% NBF fixed canine brain sections. Embedded in paraffin and sectioned at 4 microns on plus slides with distilled water in the water b