Re: [base] spot images error

2008-03-27 Thread Lepp, Dion
Thanks, that was the problem.  It is working now.  

One quirk/bug I came across is that after re-creating spot images for a
bioassay, the images from the original creation still remain.  Logging
in and out of Base does not correct this.  However, refreshing the
webpage (which automatically logs you out) and then logging back in
causes the new images to be displayed.

Dion
 
 Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire
Canada

-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Nicklas
Nordborg
Sent: Thursday, March 27, 2008 2:59 AM
To: BASE ML
Subject: Re: [base] spot images error


Lepp, Dion wrote:
 I am uing BASE 2.4.5 (build #3894; schema #40)
 
 Dion
  
  Agriculture and Agri-Food Canada - Agriculture et Agroalimentaire
 Canada
 
 -Original Message-
 From: [EMAIL PROTECTED]
 [mailto:[EMAIL PROTECTED] On Behalf Of
Nicklas
 Nordborg
 Sent: Wednesday, March 26, 2008 1:16 PM
 To: BASE ML
 Subject: Re: [base] spot images error
 
 
 Lepp, Dion wrote:
 I'm trying to create spot images from a tiff file (single layer from 
 Scanarray software) and keep receiving an error with the following
 stack 
 trace.  The image is 5 um, and I'm using the parameters given in the 
 .gpr file (xscale= 5, yscale=5, xoffset= 1155, yoffset = 1577) and a 
 spot size = 32.  I've tried a number of variations on these values
 with 
 no luck.  Any ideas?

It happens because there is at least one spot which has a null value in 
it's X coordinate. It is not possible to extract the spot images unless 
the data contains X and Y coordinates and those spots should of course 
be ignored. We'll fix this in 2.6.2.

/Nicklas


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Re: [base] spot images error

2008-03-27 Thread Nicklas Nordborg
Lepp, Dion wrote:
 Thanks, that was the problem.  It is working now.  
 
 One quirk/bug I came across is that after re-creating spot images for a
 bioassay, the images from the original creation still remain.  Logging
 in and out of Base does not correct this.  However, refreshing the
 webpage (which automatically logs you out) and then logging back in
 causes the new images to be displayed.

I think that is your browser that caches the images. The reason that 
logging in and out helps is that the session ID is part of the URL to 
the images which means that they are reloaded by the browser.

/Nicklas

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[base] spot images error

2008-03-26 Thread Lepp, Dion
I'm trying to create spot images from a tiff file (single layer from Scanarray 
software) and keep receiving an error with the following stack trace.  The 
image is 5 um, and I'm using the parameters given in the .gpr file (xscale= 5, 
yscale=5, xoffset= 1155, yoffset = 1577) and a spot size = 32.  I've tried a 
number of variations on these values with no luck.  Any ideas?

 

java.lang.NullPointerException
at net.sf.basedb.core.SpotImages.createSpotImages(SpotImages.java:487)
at net.sf.basedb.plugins.SpotImageCreator.run(SpotImageCreator.java:148)
at 
net.sf.basedb.core.PluginExecutionRequest.invoke(PluginExecutionRequest.java:89)
at net.sf.basedb.core.InternalJobQueue$JobRunner.run(InternalJobQueue.java:421)
at java.lang.Thread.run(Thread.java:595)

 

 

Thanks,

 

Dion

 


Dion Lepp, M.Sc.
Guelph Molecular Biology Research Unit
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
Telephone/Téléphone: 519-780-8090
Facsimile/Télécopieur: 519-824-2600
93 Stone Road West
Guelph, Ontario
N1G 5C9
[EMAIL PROTECTED] mailto:[EMAIL PROTECTED] 

 

 

 

 

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