[ccp4bb] Good NMR Board
Morning every body! Does anybody know a board for NMR releated questions which has a quality like this? Thx for your answers
Re: [ccp4bb] Good NMR Board
I guess you can use this one - it certainly isnt restricted to crystallographic Qs.. Eleanor Justin Schmitz wrote: Morning every body! Does anybody know a board for NMR releated questions which has a quality like this? Thx for your answers
Re: [ccp4bb] Good NMR Board
There is an NMR Board. Problem is that you have to abide by its Rules: First Rule: You do not talk about the NMR board. Second Rule: You DO NOT talk about the NMR board. James Justin Schmitz wrote: Morning every body! Does anybody know a board for NMR releated questions which has a quality like this? Thx for your answers
Re: [ccp4bb] Good NMR Board
CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK wrote on 02/08/2007 06:34:44 AM: Thanks for the links to the boards! But I think you could also answer my question. I need a way to remove gas, solved in my protein sample after using pressure filtration. My problem is the small sample volume of 400 mikroliters. greets Justin Hi Justin - I was also going to suggest the MicroCal degasser; of course you have to have the equipment already. Some other, lower-tech possibilities: - Put the sample in a 15-ml Falcon tube. Attach house vacuum to the top of the tube - they can usually take it without cracking. Tap tube vigorously on the benchtop to nucleate the bubbles. - A brief spin in a speedvac might work - just don't leave it in or you'll dry the sample out. - Don't use pressure filtration. Try a small-volume centrifugal filter instead. Like these: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=82031-362inE=1highlight=82031-362 Disclaimer: I haven't tried the first two methods for this purpose. The first method is similar to how I degassed my calorimetry samples before Microcal introduced its degasser. You might want to try it on a dry tube first... - Matt -- Matthew Franklin , Ph.D. Senior Scientist, ImClone Systems 180 Varick Street, 6th floor New York, NY 10014 phone:(917)606-4116 fax:(212)645-2054 Confidentiality Note: This e-mail, and any attachment to it, contains privileged and confidential information intended only for the use of the individual(s) or entity named on the e-mail. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that reading it is strictly prohibited. If you have received this e-mail in error, please immediately return it to the sender and delete it from your system. Thank you.
Re: [ccp4bb] Good NMR Board
Put it in 1 ml syringe, seal the end with your finger (use a piece of parafilm in between if you need it) and then draw out the plunger to create negative pressure, and your bubbles will go away. Then let it gravity-drain or very slowly push the contents into an eppindorf tube. or Speed-vac it for about 1 or 2 minutes (not long enough to lose volume). On Thu, 8 Feb 2007, Justin Schmitz wrote: Thanks for the links to the boards! But I think you could also answer my question. I need a way to remove gas, solved in my protein sample after using pressure filtration. My problem is the small sample volume of 400 mikroliters. greets Justin P.s. special thanks to James for his constructive advice!!
[ccp4bb] off-topic Apple computer question
Hi folks: Sorry this is a wee bit off topic, but since I am more likely to get a straight answer from people here, I'm going to ask.. I have 2 identical laptops. We bought both for the lab about 2 years ago. They are G4 ppc. I bought an extra half gig of memory for each at the time of purchase, but I think it is from ramjet, not Apple. Both drives failed within a few weeks of one another, making me wonder if they were really built by Ford. The second one came back from Apple today with a snotty message saying that the third-party memory had caused the problem and that they will refuse to do a repair if we ever send them a computer in the future with a third- party memory chip in it. This strikes me as absolute horse-hockey, but then again, maybe I am not aware of something I should be. Thanks. Bill
Re: [ccp4bb] off-topic Apple computer question
Hi Bill, This sounds very unusual. We have 3rd party RAM in almost all of our Macs, and I've never had a repair request denied by AppleCare. This is an allowed DIY (do it yourself) installation in most if not all their recent machines. It's common knowledge now that hard drives fail all the time, new or old - just seems to be hit or miss from all manufacturers these days and certainly not the users fault. We had an iMac hard drive fail within 3 months, and had no problem getting the repair from AppleCare. If it is AppleCare your are dealing with, call back and explain what you received back from them. If you don't get a satisfactory answer, ask to talk to a supervisor, and keep going up the chain until someone with sufficient authority can explain this message or get it repaired properly. I would also suggest running memtest, or their own extended Apple Hardware Test (usually on the discs that came in the box), to prove the memory is working fine (from bootable CD/DVD or external drive). With their own test verifying the RAM, how can they prove it caused a disk failure? In one and only one case, I had to go outside AppleCare. The best route turned out to be through the institute's procurement department. There is a single Apple higher education representative that takes all the orders from here via procurement. That turns out to be a lot of orders (i.e. money), so there is more concern about resolving problems. I wrote a detailed e-mail and received a positive reply that led to a full repair. There may also be Apple higher education account executives and engineers working with your university. They may be helpful as well. Your work has undoubtedly helped to promote Macs and probably a few sales as a result. I would suggest tactfully including this in your correspondence should it come to this. If its not AppleCare, then I hope a similar approach would also work. Best of luck, Jeff On Feb 8, 2007, at 3:04 PM, William Scott wrote: Hi folks: Sorry this is a wee bit off topic, but since I am more likely to get a straight answer from people here, I'm going to ask.. I have 2 identical laptops. We bought both for the lab about 2 years ago. They are G4 ppc. I bought an extra half gig of memory for each at the time of purchase, but I think it is from ramjet, not Apple. Both drives failed within a few weeks of one another, making me wonder if they were really built by Ford. The second one came back from Apple today with a snotty message saying that the third-party memory had caused the problem and that they will refuse to do a repair if we ever send them a computer in the future with a third- party memory chip in it. This strikes me as absolute horse-hockey, but then again, maybe I am not aware of something I should be. Thanks. Bill
[ccp4bb] Converting TNT HKL Files to MTZ using CCP4I in CCP4 6.0.2
Hi, I have a basic operational problem. I am trying to convert a pair of TNT HKL format files into an MTZ file using the CCP4I interface. (I realize this is probably not the most heavily exercised code in the package.) When I run the task I get the following output. #CCP4I VERSION CCP4Interface 1.4.4.2 #CCP4I SCRIPT LOG import #CCP4I DATE 08 Feb 2007 22:59:11 #CCP4I USER dale #CCP4I PROJECT TST #CCP4I JOB_ID 17 #CCP4I SCRATCH /tmp/dale #CCP4I HOSTNAME terbium.uoregon.edu #CCP4I PID 18342 #CCP4I TERMINATION STATUS 0 Error from script /usr/local/ccp4-6.0.2/ccp4i/scripts/import.script: can't read fo: no such variable #CCP4I TERMINATION TIME 08 Feb 2007 22:59:11 #CCP4I MESSAGE Task failed I'm afraid I'm not fluent in TCL/TK. Can anyone tell me what I should do to get this working? Thanks, Dale Tronrud
