Re: [ccp4bb] COOT (version 0.2, Jan 2007) and .FCF file
Hi Paul,coot, version 0.3.1 finally have given me the map from .fcf file, but at the begining error messages are same as version 0.2. Date: Thu, 7 Jun 2007 09:39:45 +0100 From: [EMAIL PROTECTED] Subject: Re: [ccp4bb] COOT (version 0.2, Jan 2007) and .FCF file To: CCP4BB@JISCMAIL.AC.UK On Thu, 2007-06-07 at 05:39 +, U Sam wrote: Hi would appreciate suggestion/comments. I am having a problem of opening .fcf file (created by shelx) in a new version of COOT (0.2, January 2007) ((safe_scheme_command) Error in proc: key: unbound-variable args:(#f Unbound variable: ~S (read-cif-data-with-phases) #f)) This looks a bit like problem reported by George Sheldrick and fixed in later versions - my memory is hazy about ancient coots. /usr/bin/awk exit status: 0 This is coot checking that awk is available (it's running which awk) Finally chances are that coot uses an environment variable to call coot. Sounds like a cool idea - there's a lot of recursion in coot. Not implemented yet :) So Sam, please try 0.3.1 - if that fails then I'll do something about it. Paul. _ Make every IM count. Download Windows Live Messenger and join the i’m Initiative now. It’s free. http://im.live.com/messenger/im/home/?source=TAGWL_June07
[ccp4bb]
Hi All I'm getting clusters of crystals (tiny and long needles) and I'm trying to get good single crystals. Even I'm getting the same result at low salt concentrations. I reduced the temperature to slow down the evaporation, still no change. So I'm trying with mineral oil. Here I'm getting somewhat thicker needles and the number of needles is reduced. But my drop seems to be dried out in two or three days (I dont think there is a sealing problem). Can anyone suggest me to overcome this and to get good crystals. Thankz in advance Sajid Download prohibited? No problem! To chat from any browser without download, Click Here: http://in.messenger.yahoo.com/webmessengerpromo.php
Re: [ccp4bb] DANO from PDB
I add a fake sigma column for each data column because so many programs require one. This is slightly tangential, but does anyone know of a good way to generate semi-realistic sigma values for calculated/simulated data? The best I've been able to do is borrow from an experimental dataset of the same protein (after scaling), but that doesn't work unless you've got an experimental dataset corresponding to your simulated one. I also tried a least-squares fit (following a reference I don't have in front of me...this was a while ago), which didn't result in a good fit for our data. Pete Pete Meyer Fu Lab BMCB grad student Cornell University
