Re: [ccp4bb] CNS installation

[Fulvio Saccoccia]

Dear ccp4 users,

   I succeeded to install and use CNS 1.3 on 64bit Debain-based Linux distributions. Here my report:

  

  1. install CNS and edit cns_solve_env as described by http://cns-online.org/v1.3/;

  

  2. no other modifications of cns_solve_env or .cns_solve_ens_sh
  were required; use only the first script;

  

  3. in a shell, move to the directory you want to work in and type
  /CNS_intsallation_dir/cns_solve_1.3/cns_solve_env: this will set
  all environment variables for that shell (remember to work only on
  it - csh or tcsh is required, if not present, install one);

  

  4. in the same shell, type cns_solve;

  

  5. the CNS prompt will appear;

  

  6. now, it is possible to run any .inp just typing @command (i.e.
  CNSsolve@cross_rotation.inp).

  

  cns_solve_env and cns_solve_env_sh do not run if I stay in the
  installation directory; that is, running them in
  ~/xtal/CNS/cns_solve_1.3 I still receive the message "Word too
  long". I don't know why

  

  That's all. So far, CNS runs both under Ubuntu 12.04 and Debian
  6.0.4.

  

  Thanks to all for precedent replies.

  

  Best regards

  

  Fulvio Saccoccia

  Dept. of Biochemical Sciences

  Sapienza University of Rome, Italy

  

Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal)

[Radisky, Evette S., Ph.D.]

As I recall, I used to wear latex gloves, add extra padding to the handle end 
of the wand (and other tools) with bits of tubing, and take my tools out of the 
cold room every 20 min or so to de-ice and dry them.  I didn't really have a 
choice about the cold room because it was the only place my crystals would 
grow, and my crystallization solution was 20% isopropanol which was too 
volatile to work with outside the cold room anyway.

 

Evette S. Radisky, Ph.D. 
Assistant Professor 
Mayo Clinic Cancer Center 
Griffin Cancer Research Building, Rm 310 
4500 San Pablo Road 
Jacksonville, FL 32224 
(904) 953-6372 

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Bosch, 
Juergen
Sent: Friday, July 13, 2012 8:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] harvesting in cold room (was: cryo for high salt crystal)

 

This is a very interesting topic I have to say.

 

But what I missed in this discussion is the pain you go through when freezing 
in the cold room. As the name implies it's supposed to be cold (most of the 
times). But that's not too much of an issue as you can dress up accordingly. 
The problem I always had was freezing up of the advertisement Hampton 
Magnetic Wand /advertisement and icing up towards your fingertips after some 
time when moisture from the cold room condenses and freezes. I hate wearing 
gloves when handling crystals so there was not much of a skin protection.

 

How do you guys solve this problem ?

 

Jürgen

 

..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry  Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab:  +1-410-614-4894
Fax:  +1-410-955-2926
http://lupo.jhsph.edu