[ccp4bb] Can I add PEG or Li2SO4 into protein sample for new screen?
Hi everyone, I wonder if I can add PEG or Li2SO4 into protein sample for new screen? Because optimization seems no good result, initial crystal was got in Bis-Tris, PEG8000, Li2SO4. If it is OK, then what's the concentration of PEG or Li2SO4? Best regards, Shiqing
Re: [ccp4bb] Can I add PEG or Li2SO4 into protein sample for new screen?
Hi, Shiqing, In my opinion, it is not good idea. If you want to optimize the protein buffer than you could look at thermofluor method of protein buffer optimization. BTW, have you tried seeding? 03.06.2013 10:56, Qing Shi ?: Hi everyone, I wonder if I can add PEG or Li2SO4 into protein sample for new screen? Because optimization seems no good result, initial crystal was got in Bis-Tris, PEG8000, Li2SO4. If it is OK, then what's the concentration of PEG or Li2SO4? Best regards, Shiqing -- Eugene Osipov Junior Research Scientist Laboratory of Enzyme Engineering A.N. Bach Institute of Biochemistry Russian Academy of Sciences Leninsky pr. 33, 119071 Moscow, Russia e-mail: e.m.osi...@gmail.com
[ccp4bb] MAX IV Life Science Director position
Hi, If anyone fancies becoming Life Science Director at the world's brightest light source, look no further than here: http://www.lunduniversity.lu.se/o.o.i.s?id=24914Dnr=547454Type=E /Derek Derek Logan tel: +46 46 222 1443 Associate Professor mob: +46 76 8585 707 Dept. of Biochemistry and Structural Biology www.cmps.lu.sehttp://www.cmps.lu.se Centre for Molecular Protein Science www.maxlab.lu.se/node/307http://www.maxlab.lu.se/node/307 Lund University, Box 124, 221 00 Lund, Sweden
Re: [ccp4bb] MAX IV Life Science Director position
Brighter than XFEL? Or is it going to be an XFEL? JPK On Mon, Jun 3, 2013 at 10:36 AM, Derek Logan derek.lo...@biochemistry.lu.se wrote: Hi, If anyone fancies becoming Life Science Director at the world's brightest light source, look no further than here: http://www.lunduniversity.lu.se/o.o.i.s?id=24914Dnr=547454Type=E /Derek Derek Logan tel: +46 46 222 1443 Associate Professor mob: +46 76 8585 707 Dept. of Biochemistry and Structural Biology www.cmps.lu.se Centre for Molecular Protein Science www.maxlab.lu.se/node/307 Lund University, Box 124, 221 00 Lund, Sweden -- *** Jacob Pearson Keller, PhD Looger Lab/HHMI Janelia Farms Research Campus 19700 Helix Dr, Ashburn, VA 20147 email: kell...@janelia.hhmi.org ***
Re: [ccp4bb] MAX IV Life Science Director position
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Probably brighter than an XFEL if you average over a reasonable amount of time greater than fs ;-) Tim On 06/03/2013 05:13 PM, Jacob Keller wrote: Brighter than XFEL? Or is it going to be an XFEL? JPK On Mon, Jun 3, 2013 at 10:36 AM, Derek Logan derek.lo...@biochemistry.lu.se wrote: Hi, If anyone fancies becoming Life Science Director at the world's brightest light source, look no further than here: http://www.lunduniversity.lu.se/o.o.i.s?id=24914Dnr=547454Type=E /Derek Derek Logan tel: +46 46 222 1443 Associate Professor mob: +46 76 8585 707 Dept. of Biochemistry and Structural Biology www.cmps.lu.se Centre for Molecular Protein Science www.maxlab.lu.se/node/307 Lund University, Box 124, 221 00 Lund, Sweden - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRrLVvUxlJ7aRr7hoRAueEAJ4hMgXQ1RPQ/IhCiCHTEug1ofKDKQCg6X76 eicKXxWNf20+O5eX5R4xOYM= =AmlU -END PGP SIGNATURE-
Re: [ccp4bb] MAX IV Life Science Director position
Thanks Tim! But seriously, MAX IV will, as well as being a synchrotron, have a Short Pulse Facility at the end of its linac: http://www.maxlab.lu.se/femtomax By world's brightest light source of course I loaned a management slogan, but MAX IV will be a 3 GeV facility with emittance around 0.25 nmrad, which is a factor of 4 better than PETRA-III and will, at least as long as records tend to hold (i.e. not long), have the best specs in the world: http://www.maxlab.lu.se/node/206 /Derek On 3 Jun 2013, at 17:25, Tim Gruene t...@shelx.uni-ac.gwdg.de wrote: -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Probably brighter than an XFEL if you average over a reasonable amount of time greater than fs ;-) Tim On 06/03/2013 05:13 PM, Jacob Keller wrote: Brighter than XFEL? Or is it going to be an XFEL? JPK On Mon, Jun 3, 2013 at 10:36 AM, Derek Logan derek.lo...@biochemistry.lu.se wrote: Hi, If anyone fancies becoming Life Science Director at the world's brightest light source, look no further than here: http://www.lunduniversity.lu.se/o.o.i.s?id=24914Dnr=547454Type=E /Derek Derek Logan tel: +46 46 222 1443 Associate Professor mob: +46 76 8585 707 Dept. of Biochemistry and Structural Biology www.cmps.lu.se Centre for Molecular Protein Science www.maxlab.lu.se/node/307 Lund University, Box 124, 221 00 Lund, Sweden - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -BEGIN PGP SIGNATURE- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFRrLVvUxlJ7aRr7hoRAueEAJ4hMgXQ1RPQ/IhCiCHTEug1ofKDKQCg6X76 eicKXxWNf20+O5eX5R4xOYM= =AmlU -END PGP SIGNATURE-
