Dear CCP4ers, a post-doctoral position is immediately available in my lab at the Institute of Cancer Research, Chelsea, London to study multi-subunit enzymes, transcription factors and regulatory complexes involved in RNA Polymerase III transcription (www.icr.ac.uk/alessandrovannini). The position is offered initially for 3 years but can be further extended. Expression and purification protocols from endogenous and recombinant sources have been already established in the lab and hybrid techniques (X-ray crystallography and EM) will be employed for structural characterization. Please find below details on how to apply. For informal inquiries you can contact me directly at alessandro.vann...@icr.ac.uk .
Best, Ale A post-doctoral position is available immediately in the laboratory of Dr. Alessandro Vannini, Division of Structural Biology at The Institute of Cancer Research. We are looking for highly motivated individuals with a strong interest in structural characterization of multi-subunit macromolecular complexes. We currently employ single particle electron microscopy analysis and X-ray crystallography, as well as biochemical and biophysical analysis, to elucidate the role of the RNA Polymerase III and associated factors in cancer development. The Division of Structural Biology has managed facilities for protein crystallography (Bruker Microstar and CCD detector and crystallisation robots), cryo-electron microscopy (FEI Tecnai F20 and T12), and protein production with expertise in multi-subunit expression (insect cell, yeast and bacterial expression, including a 60 L fermentor). The Division is also well equipped with equipment for biophysical analysis (e.g. ITC, fluorescence, multi-angle light scattering). Applicants should possess a PhD (or equivalent) in biochemistry or molecular biology and a sound knowledge in production and purification of macromolecular complexes for structural biology analysis. Previous experience with purification of multi-subunit complexes and/or biochemistry of protein-nucleic acid complexes would be beneficial. Previous experience in protein crystallography and/or single particle electron microscopy would be desirable. Appointment will be on Fixed Term Contract for 3 years in the first instance, with a starting salary in the range of £28,425 to £34,944 p.a. inclusive (based on previous post-doctoral experience). CV and covering letter must be submitted online at http://www.icr.ac.uk/jobsearch Job Ref. No. 1378320 Closing Date: 29th November 2013
