[ccp4bb] Graphic for rotation about axis
Where can you get hold of a graphic that indicates a rotation around the horizontal or vertical axis These occur in a lot of papers to indicate the relation between two views but I don't seem to easily be able to google how to get hold of them best wishes Nick -- Prof Nicholas H. Keep Executive Dean of School of Science Professor of Biomolecular Science Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences Birkbeck, University of London, Malet Street, Bloomsbury LONDON WC1E 7HX email n.k...@mail.cryst.bbk.ac.uk Telephone 020-7631-6852 (Room G54a Office) 020-7631-6800 (Department Office) Fax 020-7631-6803 If you want to access me in person you have to come to the crystallography entrance and ring me or the department office from the internal phone by the door
[ccp4bb] AW: [ccp4bb] Enzyme kinetics
Dear Rohit, If you want to study E1 using the E2 reaction as a read-out, you have to make sure that the E1 reaction is rate-limiting and not the E2 reaction. Ideal would be if you could purchase P1 and first map the E2 reaction with P1. Once you know the Km, Vmax and pH behavior of E2, you can calculate how much E2 you should add to make sure the E2 reaction is not rate-limiting. Alternatively, you could add varying amounts of E2 to the reaction mixture, to find out the point where the overall reaction speed no longer increases upon E2 addition, which indicates that the E1 reaction has become rate-limiting. As long as you do not know which of the two reactions is rate-limiting, it does not help to do the measurements at any specific pH. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von rohit kumar Gesendet: Freitag, 8. Mai 2015 15:23 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Enzyme kinetics Dear all, Sorry for off topic discussion. i have a doubt for the enzyme kinetic. [Inline image 2] Above is the reaction of my interest. it is a couple reaction. I want to determine the Km and Vmax for the E1 enzyme by the help of E2 enzyme by decreasing the amount of NADH (at 340 nm). if i don't know the optimum pH for E1. So is it ok, for publication point of view, to determine the Km and Vmax value of E1 enzyme at pH 7.5 ( a physiological pH) . Suppose if i determine the optimum pH of E1 by the help of E2 enzyme, that will be solely depend on the behaviour of E2 at different pH (if i am not wrong). Please suggest. Thanks in advance. -- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
Re: [ccp4bb] Enzyme kinetics
In these coupled enzyme assays, concentrations of [E1], [S], [E2] and [NADH] need to be chosen such that the rate of the second reaction is at least one to two orders of magnitude faster than the first, otherwise the measured rate -d[NADH]/dt will not be rate-limited by -d[S]/dt. Normally this is accomplished by using relatively high [E2] and [NADH] and relatively low [E1]. When varying the pH of the reaction, care must be taken to ensure that this condition is maintained, as the coupled reaction (P1 - P2) may also have a pH-rate variation. You can do a crude pH-rate profile by choosing a single concentration of [S] and assaying at various pH values to establish the approximate pH optimum. However, to establish the true pH optima you would need to establish values of kcat and kcat/Km at each pH value by measuring rates at various [S] values and fitting to the Michaelis-Menten equation (if M-M behavior is appropriate for E1). Cheers, ___ Roger S. Rowlett Gordon Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu On 5/8/2015 9:23 AM, rohit kumar wrote: Dear all, Sorry for off topic discussion. i have a doubt for the enzyme kinetic. Inline image 2 Above is the reaction of my interest. it is a couple reaction. I want to determine the Km and Vmax for the E1 enzyme by the help of E2 enzyme by decreasing the amount of NADH (at 340 nm). if i don't know the optimum pH for E1. So is it ok, for publication point of view, to determine the Km and Vmax value of E1 enzyme at pH 7.5 ( a physiological pH) . Suppose if i determine the optimum pH of E1 by the help of E2 enzyme, that will be solely depend on the behaviour of E2 at different pH (if i am not wrong). Please suggest. Thanks in advance. -- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
[ccp4bb] Enzyme kinetics
Dear all, Sorry for off topic discussion. i have a doubt for the enzyme kinetic. [image: Inline image 2] Above is the reaction of my interest. it is a couple reaction. I want to determine the Km and Vmax for the E1 enzyme by the help of E2 enzyme by decreasing the amount of NADH (at 340 nm). if i don't know the optimum pH for E1. So is it ok, for publication point of view, to determine the Km and Vmax value of E1 enzyme at pH 7.5 ( a physiological pH) . Suppose if i determine the optimum pH of E1 by the help of E2 enzyme, that will be solely depend on the behaviour of E2 at different pH (if i am not wrong). Please suggest. Thanks in advance. -- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
Re: [ccp4bb] Graphic for rotation about axis
Hi Nick, These symmetry element fonts at the IUCr website might be useful or at least inspirational: http://www.iucr.org/resources/symmetry-font Best regards, Blaine Blaine Mooers, Ph.D. Assistant Professor Director of the Laboratory of Biomolecular Structure and Function Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- Small Angle Scattering webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-scattering-links-27aug2014.html?sfvrsn=0 X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Nicholas Keep [n.k...@mail.cryst.bbk.ac.uk] Sent: Friday, May 08, 2015 9:37 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Graphic for rotation about axis Where can you get hold of a graphic that indicates a rotation around the horizontal or vertical axis These occur in a lot of papers to indicate the relation between two views but I don't seem to easily be able to google how to get hold of them best wishes Nick -- Prof Nicholas H. Keep Executive Dean of School of Science Professor of Biomolecular Science Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences Birkbeck, University of London, Malet Street, Bloomsbury LONDON WC1E 7HX email n.k...@mail.cryst.bbk.ac.uk Telephone 020-7631-6852 (Room G54a Office) 020-7631-6800 (Department Office) Fax 020-7631-6803 If you want to access me in person you have to come to the crystallography entrance and ring me or the department office from the internal phone by the door
Re: [ccp4bb] Graphic for rotation about axis
These can be created from almost any graphics program, including powerpoint (see attached for a few simple examples). P. Shing Ho, Ph.D. Professor Chair Biochemistry Molecular Biology 1870 Campus Delivery Colorado State University Fort Collins, CO 80523-1870 970-491-0569 (phone) On 5/8/15, 12:43 PM, Mooers, Blaine H.M. (HSC) blaine-moo...@ouhsc.edu wrote: Hi Nick, These symmetry element fonts at the IUCr website might be useful or at least inspirational: http://www.iucr.org/resources/symmetry-font Best regards, Blaine Blaine Mooers, Ph.D. Assistant Professor Director of the Laboratory of Biomolecular Structure and Function Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology /faculty/blaine-mooers-ph-d- Small Angle Scattering webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/sm all-angle-scattering-links-27aug2014.html?sfvrsn=0 X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology /department-facilities/macromolecular-crystallography-laboratory From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Nicholas Keep [n.k...@mail.cryst.bbk.ac.uk] Sent: Friday, May 08, 2015 9:37 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Graphic for rotation about axis Where can you get hold of a graphic that indicates a rotation around the horizontal or vertical axis These occur in a lot of papers to indicate the relation between two views but I don't seem to easily be able to google how to get hold of them best wishes Nick -- Prof Nicholas H. Keep Executive Dean of School of Science Professor of Biomolecular Science Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences Birkbeck, University of London, Malet Street, Bloomsbury LONDON WC1E 7HX email n.k...@mail.cryst.bbk.ac.uk Telephone 020-7631-6852 (Room G54a Office) 020-7631-6800 (Department Office) Fax 020-7631-6803 If you want to access me in person you have to come to the crystallography entrance and ring me or the department office from the internal phone by the door Arrows.pptx Description: Arrows.pptx
Re: [ccp4bb] Graphic for rotation about axis
Dear Nick, if I understand correctly, you mean anything like the attached file? I created this one with xfig, my favourite vector graphics editor. Best, Tim On 05/08/2015 04:37 PM, Nicholas Keep wrote: Where can you get hold of a graphic that indicates a rotation around the horizontal or vertical axis These occur in a lot of papers to indicate the relation between two views but I don't seem to easily be able to google how to get hold of them best wishes Nick -- -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A rot.pdf Description: Adobe PDF document signature.asc Description: OpenPGP digital signature
[ccp4bb] Your abstract can still be considered for an oral presentation at the American Crystallographic Assn Annual meeting
Hi CCP4 users, A message from the American Crystallographic Association! YES! We are still accepting abstracts. No late fee. ACA Annual Meeting • Philadelphia, PA • July 25 - 29, 2015 Submit now to be considered for an oral presentation. Once all the lectures are scheduled, abstracts will continue to be accepted for poster presentations. See: http://www.AmerCrystalAssn.org All the best, Tom T Vice-President, ACA
Re: [ccp4bb] Graphic for rotation about axis
Thanks, these are great! I installed the ttf font on a windows box, and the symbols are available in MSWord and Photoshop (and presumably powerpoint and everything else). No more designing my own symbols in photoshop! eab On 05/08/2015 02:43 PM, Mooers, Blaine H.M. (HSC) wrote: Hi Nick, These symmetry element fonts at the IUCr website might be useful or at least inspirational: http://www.iucr.org/resources/symmetry-font Best regards, Blaine Blaine Mooers, Ph.D. Assistant Professor Director of the Laboratory of Biomolecular Structure and Function Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: blaine-moo...@ouhsc.edu Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- Small Angle Scattering webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-scattering-links-27aug2014.html?sfvrsn=0 X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Nicholas Keep [n.k...@mail.cryst.bbk.ac.uk] Sent: Friday, May 08, 2015 9:37 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Graphic for rotation about axis Where can you get hold of a graphic that indicates a rotation around the horizontal or vertical axis These occur in a lot of papers to indicate the relation between two views but I don't seem to easily be able to google how to get hold of them best wishes Nick -- Prof Nicholas H. Keep Executive Dean of School of Science Professor of Biomolecular Science Crystallography, Institute for Structural and Molecular Biology, Department of Biological Sciences Birkbeck, University of London, Malet Street, Bloomsbury LONDON WC1E 7HX email n.k...@mail.cryst.bbk.ac.uk Telephone 020-7631-6852 (Room G54a Office) 020-7631-6800 (Department Office) Fax 020-7631-6803 If you want to access me in person you have to come to the crystallography entrance and ring me or the department office from the internal phone by the door
[ccp4bb] Two tenure track research group leader positions - ZIK HALOmem - University of Halle-Wittenberg, Germany
Since its foundation in 2008, the *Centre for Innovation Competence (ZIK) HALOmem*at the Martin Luther University Halle-Wittenberg in Germany (www.halomem.de http://www.halomem.de) has devoted itself to the structural characterisation of membrane proteins together with analysis of the role played by the surrounding membrane. HALOmem is expanding its expertise platform with the establishment of *two new independent research groups*, working and collaborating within the stimulating multidisciplinary environment that has established Halle as an internationally recognised centre for pure and applied protein biochemistry, biotechnology and biophysics. HALOmem and the German Federal Ministry of Education and Research (BMBF) seek highly motivated and outstanding junior scientists for the positions of: *Research Group Leader “Cryo-Electron Microscopy of Membrane Protein Complexes”* The successful candidate will establish a team to study membrane protein complexes using cryo-electron microscopy (single particle reconstruction and/or tomography). The applicant will have trained in membrane protein biochemistry and/or cryo-EM, with documented experience in these fields at postgraduate and postdoctoral level. The candidate will have unrestricted access to the 300 kV JEOL JEM-3200FSC equipped with field emission gun, cryo-stage, in-line energy filter and GATAN K2 direct electron detector that are presently being installed. *Research Group Leader “Biophysical Characterisation of Medically Relevant Membrane Proteins”* The successful candidate will establish a team to develop technologies for the biophysical characterisation of membrane proteins and the analysis of their interactions with protein and synthetic ligands. The applicant will have received training in protein ligand interactions / membrane protein biochemistry covering a wide array of methods. Documented experience in these fields at postgraduate and postdoctoral level is expected; experience in an industrial environment is welcome. Furthermore, applicants should ideally have experience in: * Working in an international scientific environment * Project coordination and leadership * Obtaining third party funding * Interacting within an interdisciplinary milieu Each group will receive funding from the BMBF, including an additional four co-workers and consumables for five years, as well as laboratory equipment necessary for the project. Implementation of the extended ZIK HALOmem Strategy Concept is a requirement for both group leaders, and successful candidates are expected to contribute to establishment of the Research training programme “Halle School of Molecular Life Sciences (HaMol)”. Group leaders may be awarded the status of Junior Professor, in which case active participation in academic and administrative duties within the University is expected according to the /Hochschulgesetz Sachsen-Anhalt/(§ 34 HSG-LSA), with corresponding requirements for recruitment according to § 35 HSG-LSA. Junior Professorships are awarded initially for a period of three years, after which a further three years may be granted upon positive evaluation. In such a case, both positions offer the possibility of subsequent tenure at the Martin Luther University, which may or may not be submitted for tender depending on the success of the group leader. The Martin Luther University Halle-Wittenberg strives to promote equal opportunities in science. Female and disabled applicants, qualified according to the above criteria, will be given preference over other candidates with equivalent relevant qualifications. Salary scale is subject to legal and budgetary policies of the University and candidates are expected to start by the beginning of 2016. The University provides arrangements for coordinating occupation and family. Applications should be addressed to _both_ZIK HALOmem _and_the Project Management Organisation Jülich (representing the BMBF) with the subject headline “ZIK HALOmem II”: *ZIK HALOmem: Prof. Milton T. Stubbs, **stu...@biochemtech.uni-halle.de* *Organisation Jülich: **Dr. Christoph Wannek, **c.wan...@fz-juelich.de* *Application deadline: 12*^*th* *June 2015* *Interviews are planned from 6*^*th* *– 9*^*th* *July 2015* *For further information please contact:* i...@halomem.de; http://www.halomem.de *For further details on the application requirements please see: * http://www.unternehmen-region.de/de/374.php HALOmem_en 8p138x255 06-5.pdf Description:
