[ccp4bb] 9th workshop on Neutron Scattering Applications in Structural Biology

[Meilleur, Flora]

First announcement

9th Workshop on Neutron Scattering Applications in Structural Biology
Oak Ridge, TN. June 11 - June 15, 2018

Detailed information can be found on the workshop web page: 
https://conference.sns.gov/biologyneutrons18/
Application deadline: April 30, 2018

The workshop on Neutron Scattering Applications in Structural Biology aims at 
enabling structural biologists to fully exploit the latest instrumentation and 
software development at the SNS and HFIR facilities at Oak Ridge National 
Laboratory. Attendees will participate in lectures and tutorials focusing 
exclusively on neutron techniques applied in structural biology. The workshop 
is designed for graduate students, post-doctoral fellows and faculty new to or 
with limited experience of neutron scattering.

Flora Meilleur, Ph. D
Associate Professor, Molecular and Structural Biochemistry
North Carolina State University
Neutron Scattering Scientist, Neutron Sciences Directorate
Oak Ridge National Laboratory

Re: [ccp4bb] protein quasicrystals?

[Prem Prakash]

Hi Yu,

Microseeding along with additive screening could give a better result,
because I encountered a situation once like this. Try higher concentration
of protein with bigger drop volume during crystallisation.

Good luck

Prem

On Wed, Feb 14, 2018 at 4:16 PM, Huyton, Trevor  wrote:

> Dear Yu,
>
>
>
> If the crystals are grown from salt then put the drop over water for a few
> hours and dissolve them, then put it back over the reservoir solution and
> recrystallize.
>
> Sometimes you can get better crystals this way.
>
> You could also try melting them with temperature and recrystallizing
>
>
>
> Best
>
>
>
> Trevor
>
>
>
> *From: *CCP4 bulletin board  on behalf of Yu Qiu <
> yu@sanofi.com>
> *Reply-To: *"yu@sanofi.com" 
> *Date: *Tuesday 13 February 2018 at 16:11
> *To: *"CCP4BB@JISCMAIL.AC.UK" 
> *Subject: *[ccp4bb] protein quasicrystals?
>
>
>
> Hi,
>
>
>
> I have been trying to crystallize a protein complex and keep getting
> sphere shape crystals. The diffraction is around 3 angstrom, but looks like
> multiple lattices. I am wondering if it could be a quasi crystal? Is there
> anyone has such experience?
>
>
>
> Thanks,
>
> Yu
>



-- 
With kind regards,

Prem Prakash
PhD Research Scholar
Protein Crystallography Lab
Biosciences and Bioengineering
IIT Bombay

Re: [ccp4bb] protein quasicrystals?

[Huyton, Trevor]

Dear Yu,

If the crystals are grown from salt then put the drop over water for a few 
hours and dissolve them, then put it back over the reservoir solution and 
recrystallize.
Sometimes you can get better crystals this way.
You could also try melting them with temperature and recrystallizing

Best

Trevor

From: CCP4 bulletin board  on behalf of Yu Qiu 

Reply-To: "yu@sanofi.com" 
Date: Tuesday 13 February 2018 at 16:11
To: "CCP4BB@JISCMAIL.AC.UK" 
Subject: [ccp4bb] protein quasicrystals?

Hi,

I have been trying to crystallize a protein complex and keep getting sphere 
shape crystals. The diffraction is around 3 angstrom, but looks like multiple 
lattices. I am wondering if it could be a quasi crystal? Is there anyone has 
such experience?

Thanks,
Yu