[ccp4bb] 23rd Annual SCSB Symposium, April 28th, Galveston Texas

[Mark A. White]

You and your colleagues are cordially invited to join us for the 23rd
Annual Structural Biology Symposium to be held at Levin Hall on the
University of Texas Medical Branch Campus on beautiful Galveston Island
on Saturday, April 28, 2018.  The meeting is organized by the Sealy
Center for Structural Biology & Molecular Biophysics and co-sponsored by
the Keck Center for Computational & Structural Biology.  This year’s
speakers include: 


   Grant Jensen, Ph.D.,   California Institute of Technology
 “Progress and Challenges in Electron Cryotomography” 

 Ayyalusamy Ramamoorthy, Ph.D., University of Michigan
“Membrane-Assisted Dynamic Protein-Protein Interactions by NMR"

Andrew Routh, Ph.D.,  University of Texas Medical Branch
   “Parallel ClickSeq and Nanopore Deep-Sequencing Characterizes the
  Emergence and Evolution of Defective Viral Genomes” 

 Joan-Emma Shea, Ph.D., University of California Santa Barbara
  “Binding and Assembly of Intrinsically Disordered Peptide” 

   Yizhi Tao, Ph.D.,  Rice University
“The Tail of a Nematode Virus” 

   Adam Zlotnick, Ph.D.,  Indiana University
  “Multiple Mechanisms for Assembly-Directed HBV Antivirals” 


 To register or for more information, 
  visit http://scsb.utmb.edu/symposium


-- 
Yours sincerely, 

Mark A. White, Ph.D. 
Associate Professor of Biochemistry and Molecular Biology, 
Manager, Sealy Center for Structural Biology and Molecular Biophysics 
Macromolecular X-ray Laboratory, 
Basic Science Building, Room 6.658A 
University of Texas Medical Branch 
Galveston, TX 77555-0647 
Tel. (409) 747-4747 
mailto://mawh...@utmb.edu 
http://xray.utmb.edu 

QQ: "For a man to conquer himself is the first and noblest of all
victories." 
- Plato 

[ccp4bb] Job opening at Evotec UK Ltd.

[Hole, Alison]

Dear All,

There is an opportunity to join the Structural Biology team at Evotec based in 
the U.K as a Senior Scientist (Crystallography). Please see the advert in the 
link below. Interested candidates should apply directly through our website. 
Please do not send applications through to me directly. Closing date is April 
20th and applicants must be eligible to work in UK. The position is for a 6 
month contract.

https://recruitment.evotec.com/VacancyDetails.aspx?FromSearch=True=QSAukis1tlc==616

Best regards,
Alison Hole

STATEMENT OF CONFIDENTIALITY.

This email and any attachments may contain confidential, proprietary, 
privileged and/or private information.  
If received in error, please notify us immediately by reply email and then 
delete this email and any attachments from your system. Thank you!


Evotec (UK) Ltd is a limited company registered in England and Wales. 
Registration number:2674265. Registered office: 114 Innovation Drive, Milton 
Park, Abingdon, Oxfordshire, OX14 4RZ, United Kingdom.

[ccp4bb] COMPPÅ symposium - register now!

[Oliver Clarke]

Hi all,

The deadline to register for the COMPPÅ symposium (covering production, 
structure determination, functional analysis and engineering of membrane 
proteins) is coming up in less than a month! 

Take a look at the attached flyer for confirmed speakers - I think it’s a 
pretty top notch line up and will be a great meeting.

The meeting will be held here at Columbia University, in the Roy and Diana 
Vagelos Education center, June 17-19 2018.

See you there!

Registration: https://www.comppaa.org/comppaa-symposium-2018 


Flyer: 
https://docs.wixstatic.com/ugd/d999b5_3c8dfb411cff4ea39277fbfb352ca402.pdf

Cheers

Oli Clarke

Re: [ccp4bb] Refmac: problem with anisotropic refinement of heavy atoms.

[M T]

Sorry, I moved the image before the sending of the mail and then I was not
attached.

2018-04-04 11:26 GMT+02:00 M T :

> Hello,
>
> I am refining a structure at 2.1Å, using Refmac and manual constructions
> in coot.
> I have few Ca, Cl and Mg in my structure and some of them have a clearly
> anisotropic distribution, then I decided to use ANISOU line in pdb file
> only for these atoms.
> In refinement parameters of Refmac I set "mixed (isotropic/anisotropic)
> temperature factors".
> And I have a problem with some of them which are obviously badly refined
> (see attached picture).
>
> What could be the reason of that? What should I badly set?
>
> Thank you for your help.
>

[ccp4bb] 3. User Call for MAX IV BioMAX beamtime, period Oct. 2018-June 2019, deadline April 27

[Uwe Müller]

Dear all,
MAX IV in Lund, Sweden has opened the 3. user call to receive proposals 
requesting beamtime at BioMAX for the period Oct 2017-June 2019 until April 27.
In order to submit a new proposal, please visit this page:
https://www.maxiv.lu.se/users/open-calls/

BioMAX started its user operation in March 2017 and could successfully operate 
already around 50 proposals within a limited number of user operations. Within 
the next beam period we will ramp up the operations and will offer BioMAX to 
the broad international user community. The beam line is under continuous 
commissioning and will offer the following features during the next user period:

• 20x5 µm^2 beam focus at sample position (can be further reduced by apertures 
down to 5x5 µm^2)
• Around 7-9 x 10^12 Phot/sec at 200 mA ring filling at sample position
• Average exposure time between 8-10 msec / frame, corresponding to 
10-30% transmission
• Tuneable energy range 5-20 keV
• Initial sample changer capability using SPINE samples and UNIPucks possible 
with ISARA sample changer
• Eiger 16M hybrid pixel detector 130 Hz frame rate for 16M mode
• MD3 micro diffractometer
• HClab crystal dehydration device with fast nozzle changer for crystal 
dehydration and room temperature data collections
• MXCuBE3 experiment control software & ISPyB database support
• Automatic data processing during data collection
• Standard crystallographic software installed
• >1000 core HPC environment availalbe
• Sample preparation laboratory for crystal handling

MAXIV is member of the EU project iNEXT, which also supports travel and 
accommodation for international user groups (See also: 
http://www.inext-eu.org). On campus, we will have a guesthouse available, which 
can be booked via MAXIV before the beamtime as well.
We are looking forward for your beamtime proposals and the collaboration during 
your stay at MAX IV.

Please take also into account the long EBS-upgrade shutdown of the ESRF!
See: 
http://www.esrf.eu/home/UsersAndScience/Publications/Highlights/highlights-2015/introduction.html

Uwe & the MAX IV MX-team!


Uwe Mueller
Group manager Macromolecular Crystallography
BioMAX beam line manager

MAX IV Laboratory
Lund University
Postal address: P.O. Box 118, SE-221 00 Lund, Sweden
Visiting address: Fotongatan 2, 225 94 Lund, Sweden
Mobile:  +46(0)730-655268
E-mail: uwe.mul...@maxiv.lu.se
Url: www.maxiv.se
VAT No.: SE 2021 0032 1101

[ccp4bb] Refmac: problem with anisotropic refinement of heavy atoms.

[M T]

Hello,

I am refining a structure at 2.1Å, using Refmac and manual constructions in
coot.
I have few Ca, Cl and Mg in my structure and some of them have a clearly
anisotropic distribution, then I decided to use ANISOU line in pdb file
only for these atoms.
In refinement parameters of Refmac I set "mixed (isotropic/anisotropic)
temperature factors".
And I have a problem with some of them which are obviously badly refined
(see attached picture).

What could be the reason of that? What should I badly set?

Thank you for your help.

Re: [ccp4bb] Sulphur SAD at home source

[Kay Diederichs]

Hi Manoj,

it depends on crystal and hardware (detector, beam, goniometer) quality as well 
as on the correct strategy whether you will be able to solve the structure with 
sulfur SAD. 6 sulfur in 12 kDa should be doable, if the crystals diffract to 
2.5A or better. Others have given good advice or links on these topics.

My only addition is: you should not assume that there is no radiation damage on 
the home source, rather, it is very noticeable - see e.g. Sarma and Karplus, 
"In-house sulfur SAD phasing: a case study of the effects of data quality and 
resolution cutoffs" Acta Cryst. (2006). D62, 707–716. This means: 
high-multiplicity data from a single crystal are not guaranteed to be 
isomorphous. If you have many crystals, however, you could merge several data 
sets.

best wishes,

Kay

On Tue, 3 Apr 2018 10:26:50 -0400, Manoj Saxena  wrote:

>Hi All,
>
>I am writing to seek advice on doing  sulphur SAD data collection
>at Cu based home source for a protein that is 12 KDa and has 6 S atoms.
>I have seen some links online and some references but would be grateful if
>you can share your know-how for success with this.
>Like what multiplicity of data would be good to aim for and
>data processing tips.
>Inputs from people who have tried and failed would also be highly
>appreciated.
>
>Thank you
>Manoj Saxena
>University of Puerto Rico
>