Re: [ccp4bb] TEV Protease in low reducing agent?

[Chun Luo]

Hi Nicola,

Our TurboTEV (http://accelagen.com/TurboTEV-datasheet.htm) is very stable and 
does not need additional reducing agent for its activity. The storage buffer 
has 1 mM TCEP. It'll be likely <0.1 mM in your digestion. You can get it from 
our European distributors or directly from Accealgen. Sigma probably sells it 
in Europe as well. TurboTEV is shipped with gel packs and can be stored at -20 
for long time.

Cheers,
Chun
Accelagen

-Original Message-
From: CCP4 bulletin board  On Behalf Of Nicola Evans
Sent: Wednesday, February 13, 2019 10:46 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] TEV Protease in low reducing agent?

I am interested in purifying proteins with 1 or 2 disulphide bonds, and have 
been using enterokinase to cleave off N-terminal tags but we have had issues 
with poor cleavage and want to try TEV cleavage instead. However TEV protease 
is usually kept in a high amount of DTT and I am concerned about reducing the 
disulphide bonds in my purified proteins. I have used TEV protease before with 
0.25mM TCEP and it worked well. Is there a way to use TEV with very little or 
no reducing agent? Perhaps by optimising conditions such as adding glycerol? We 
were thinking of buying in commercial TEV protease so any advice on that is 
also welcomed (is there any merit, except cost, to making it ourselves?)



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[ccp4bb] Re : [ccp4bb] Can anyone run refmac from the WINDOWS command prompt?

[Philippe BENAS]

Hi Kevin,

Since CCP4 v.7 there is an issue with the term Windows. I was used to run any 
CCP4 program from anywhere on my disks with v. 6.x but with v.7x you have to 
move every needed fiies to C:\CCP4\ccp4-7.0 (or something like this): Indeed 
where the shortcut of the CCP4 consol is located.

Some environment variables are not well set. Hum... I have to confess I wanted 
to do that during some spare time. The trouble is I never found spare time 
since the v.7.0 release...

HTH and all the best,
Philippe


Philippe BENAS, Ph.D.

ARN UPR 9002 CNRS
IBMC Strasbourg
15, rue René Descartes
F-67084 STRASBOURG cedex
+33.3.8841.7109

E-mails: p.be...@ibmc-cnrs.unistra.fr, philippe_be...@yahoo.fr
URLs:   http://www-ibmc.u-strasbg.fr/ , http://www-ibmc.u-strasbg.fr/spip-arn/





En date de : Mer 13.2.19, Kevin Cowtan 
<2ba34e97fcaf-dmarc-requ...@jiscmail.ac.uk> a écrit :

 Objet: [ccp4bb] Can anyone run refmac from the WINDOWS command prompt?
 À: CCP4BB@JISCMAIL.AC.UK
 Date: Mercredi 13 février 2019, 12h29
 
 Hi! Can anyone run refmac from the
 WINDOWS command prompt?
 I've been experimenting with
 various combinations of environment variables, but I always
 get an error. The one instance of this error on google is an
 unanswered CCP4BB question.
 Dictionary path has not
 been definedCheck the environment
 variable CLIBD_MONCurrent value of
 CLIBD_MON is
 C:\Apps\CCP4-7\7.0\lib\data\monomersIt should be set to wherever_dict/dic/===> 
Error: Wrong path for the dictionary
 files Refmac:  Wrong path for the dictionary
 files Refmac:  Wrong path for the
 dictionary files
 The directory
 C:\Apps\CCP4-7\7.0\lib\data\monomers
 contains the following:
  Directory of
 C:\Apps\CCP4-7\7.0\lib\data\monomers
 12/02/2019  15:42             
 .12/02/2019  15:42           
   ..12/02/2019  15:38         
     012/02/2019  15:39       
       112/02/2019  15:39     
         212/02/2019  15:39   
           312/02/2019  15:39 
             412/02/2019 
 15:39             
 512/02/2019  15:39           
   612/02/2019  15:39         
     712/02/2019  15:40       
       812/02/2019  15:40     
         912/02/2019  15:40   
           a12/02/2019  15:40 
             b12/02/2019 
 15:40             
 c28/11/2016  21:43             7,640
 COPYING12/02/2019  15:40       
       d28/11/2016  21:43           
  3,553 dnarna_basepairs.txt28/11/2016  21:43 
            3,553
 dnarna_basepairs_2.txt28/11/2016  21:43     
        5,248 dnarna_params.txt12/02/2019 
 15:40             
 e27/04/2018  10:59           105,193
 ener_lib.cif28/11/2016  23:06         
  105,136 ener_one_lib.cif12/02/2019  15:40   
           f12/02/2019  15:40 
             g12/02/2019 
 15:41             
 h12/02/2019  15:41           
   i12/02/2019  15:41         
     j12/02/2019  15:41       
       k12/02/2019  15:41     
         l12/02/2019  15:41   
           list12/02/2019 
 15:41             
 m28/11/2016  21:43         1,372,146
 mon_lib_ind.cif28/11/2016  21:43       
  6,490,087 mon_lib_ind2.cif28/11/2016  23:06 
              120
 mon_lib_one_ind.cif28/11/2016  23:06       
        396 mon_lib_one_ind2.cif12/02/2019 
 15:41             
 n31/08/2018  15:52            35,312
 new_entries.txt31/08/2018  15:52         
   23,685 not_replaced_entries.txt12/02/2019 
 15:41             
 o12/02/2019  15:41           
   p28/11/2016  21:43         2,490,954
 pdb_alt_names.txt28/11/2016  21:43         
    7,794 pdb_v2to3.py12/02/2019  15:41   
           q12/02/2019  15:41 
             r31/08/2018 
 15:52            29,731
 replaced_entries.txt31/08/2018  15:52       
        754 revert_list.txt12/02/2019 
 15:41             
 s12/02/2019  15:42           
   t28/11/2016  21:43               557
 template_link.pdb12/02/2019  15:42   
           u12/02/2019  15:42 
             v12/02/2019 
 15:42             
 w31/08/2018  15:52                24
 windows_reserved_words.txt12/02/2019  15:42   
           x12/02/2019  15:42 
             y12/02/2019 
 15:42              z-- 
 Professor Kevin
 CowtanYork Structural Biology
 Laboratory / Department of Chemistry
 University of York, Heslington, York, YO10 5DD,
 UK
 https://www.york.ac.uk/chemistry/staff/academic/a-c/kcowtan/
 EMAIL DISCLAIMER: http://www.york.ac.uk/docs/disclaimer/email.htm
 
 
 
 
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Re: [ccp4bb] TEV Protease in low reducing agent?

[Zhijie Li]

Hi Nicola,

0.1-1mM Cysteine, GSH or BME will work fine.  You can also try using very fresh 
TEV without reducing reagent (or store it with BME and remove the reducing 
reagent by some column just before use). Depending on how concentrated the 
stock is, diluting it 3-5x could also sufficiently reduce the redox potential 
of the solution. Another thing to try is to add some GS-SG to consume the DTT 
if it is already there.
TEV is not a very stable protease due to both oxidation and aggregation. So 
yes, there is a strong reason to make it in the lab. We can usually make 
20-40mg crude TEV from a liter of LB culture.

Zhijie

> On Feb 13, 2019, at 1:47 PM, Nicola Evans 
> <251ca3b4615e-dmarc-requ...@jiscmail.ac.uk> wrote:
> 
> I am interested in purifying proteins with 1 or 2 disulphide bonds, and have 
> been using enterokinase to cleave off N-terminal tags but we have had issues 
> with poor cleavage and want to try TEV cleavage instead. However TEV protease 
> is usually kept in a high amount of DTT and I am concerned about reducing the 
> disulphide bonds in my purified proteins. I have used TEV protease before 
> with 0.25mM TCEP and it worked well. Is there a way to use TEV with very 
> little or no reducing agent? Perhaps by optimising conditions such as adding 
> glycerol? We were thinking of buying in commercial TEV protease so any advice 
> on that is also welcomed (is there any merit, except cost, to making it 
> ourselves?)
> 
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1



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Re: [ccp4bb] TEV Protease in low reducing agent?

[Bonsor, Daniel]

You can use a redox system which should maintain disulfide bonds, but still 
offer enough reduction to give TEV active. Use 3mM reduced glutathione/0.3mM 
oxidized glutathione. Add the protein to TEV so the DTT is rapidly diluted. 

We started to make our own when it rapidly became used my nearly everyone else 
in the lab. Typically 6l of cells which produce enough TEV for a lab of 6-8 
people for about 1-1.5 yr. I believe we obtained the plasmid from David Waugh 
(https://mcl1.ncifcrf.gov/waugh_tev.html) sometime last decade. 

Best of luck,


Dan

Daniel A Bonsor PhD.
Sundberg Lab
Institute of Human Virology
University of Maryland, Baltimore
725 W Lombard Street N370
Baltimore
Maryland
MD 21201
Tel: (410) 706-7457


-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Nicola 
Evans
Sent: Wednesday, February 13, 2019 1:46 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] TEV Protease in low reducing agent?

I am interested in purifying proteins with 1 or 2 disulphide bonds, and have 
been using enterokinase to cleave off N-terminal tags but we have had issues 
with poor cleavage and want to try TEV cleavage instead. However TEV protease 
is usually kept in a high amount of DTT and I am concerned about reducing the 
disulphide bonds in my purified proteins. I have used TEV protease before with 
0.25mM TCEP and it worked well. Is there a way to use TEV with very little or 
no reducing agent? Perhaps by optimising conditions such as adding glycerol? We 
were thinking of buying in commercial TEV protease so any advice on that is 
also welcomed (is there any merit, except cost, to making it ourselves?)



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1



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[ccp4bb] TEV Protease in low reducing agent?

[Nicola Evans]

I am interested in purifying proteins with 1 or 2 disulphide bonds, and have 
been using enterokinase to cleave off N-terminal tags but we have had issues 
with poor cleavage and want to try TEV cleavage instead. However TEV protease 
is usually kept in a high amount of DTT and I am concerned about reducing the 
disulphide bonds in my purified proteins. I have used TEV protease before with 
0.25mM TCEP and it worked well. Is there a way to use TEV with very little or 
no reducing agent? Perhaps by optimising conditions such as adding glycerol? We 
were thinking of buying in commercial TEV protease so any advice on that is 
also welcomed (is there any merit, except cost, to making it ourselves?)



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1

[ccp4bb] postdoc position

[Alexandra Deaconescu]

Dear ccp4bb enthusiasts,

Please help spread the word about the postdoc position described below.

Thank you,

Alexandra


*POSTDOCTORAL POSITION*

*at Brown University, USA*

The Deaconescu Lab at Brown University has one opening for a 
postdoctoral researcher. The laboratory's interests lie primarily in 
stress responses, with particular emphasis on responses to DNA damage 
and the mechanochemistry of DNA-based motors. We utilize a combination 
of biochemical, biophysical and structural techniques. (e.g. X-ray 
crystallography, small-angle X-ray scattering, transmission electron 
microscopy). For examples of work, please see Vemu et al. /Science 
/(2018), Le TT et al. /Cell/ (2018), Deaconescu et al. /Photochemistry 
and Photobiology /(2017), Kutter et al. /JMB/ (2016), Szyk et al, /Cell/ 
(2014), Deaconescu et al., /PNAS/ (2012), Szyk et al., /NSMB/ (2012) and 
Deaconescu et al., /TIBS/ (2013).


A successful candidate should have an established track-record of 
publications in peer-reviewed journals; solid experience with protein 
biochemistry (including their purification from E.coli/yeast expression, 
functional characterization and assay development).*_Prior knowledge of 
crystallography and/or single-particle electron microscopy is highly 
desirable_*. Crystals/data at various stages of the structure 
determination process are available for immediate work. Must be highly 
motivated and work independently as well as in a team. Excellent spoken 
and written English are required. New Ph.D. graduates are encouraged to 
apply.


*Interested candidates should send a CV, one page research experience 
summary and contact information for three references to 
**_alexandra_deacone...@brown.edu 
_.*Salary and starting date are 
negotiable.Please write Postdoctoral Candidate in the e-mail subject 
header.Brown University, an Ivy League school, is located less than one 
hour away by train from Boston.Lab webpage: https://deaconesculab.com


--
Alexandra Deaconescu, B.E., Ph.D.
Assistant Professor
Brown University

Office: (401) 863-3215
Wet Lab: (401) 863-6729
Computational Lab: (401) 863-7031

For Mail:
Laboratories of Molecular Medicine
70 Ship St. GE-4
Providence, RI 02903

For Courier:
Laboratories of Molecular Medicine
Brown University
70 Ship St., Chestnut St. Loading Dock
Providence, RI 02903

Website: www.deaconesculab.com

Admin
Ms. Christina Fournier
Email: christina_fournier[at]brown.edu
Mailing Address:
Box G-E, Brown University,
Providence, RI 02912-G
Telephone: 401-863-2782

Confidentiality Notice:
This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential, proprietary and privileged 
information. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, please contact the sender 
immediately and destroy or permanently delete all copies of the original 
message.




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Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] refmac same residue different names

[Clemens Vonrhein]

Dear Herman,

On Thu, Feb 07, 2019 at 10:26:09AM +, Herman Schreuder wrote:
> I did understand your question correctly and (at least for ligands)
> the procedure I and also Diana Tomchick described, worked. However,
> I just did a test with both Refmac and Buster and it seems that
> these programs have now so far been perfected that “errors” like
> this cannot occur anymore.

You might not have seen the reply to Ed's original question on the
BUSTER discussion mailing list [1]. It is not fully automatic and
might look slightly complicated (the intention was to give the full
background information for such a problem) - so doesn't qualify as a
"native" solution that Ed was looking for.

It is of course possible to handle micro-heterogeneity in BUSTER
(and also in REFMAC - after all: most structures marked with
"MICROHETEROGENEITY" in the PDB have been refined with it). If this
becomes a more common and pressing need for our users, development
of a "native" solution would move higher up the priority list list
in the future. It is very good to hear from users (like Ed) when
something doesn't quite work as expected - even if they then decide
to switch to another package/program for a particular problem.

Cheers

Clemens, Peter & Gerard

[1] 
http://www.globalphasing.com/pipermail/buster-discuss/2019-February/thread.html

> So it seems that the poor crystallographers who have crystallized proteins 
> which are heterogeneous at certain positions, will have to switch to Phenix.



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Re: [ccp4bb] 2019 mid-range & high-end LINUX laptops for structural biology

[Jon Agirre]

Dear Domen,

I bought a Lenovo Thinkpad p72 for both MX and cryoEM work last year and I
am generally happy. The Xeon CPUs do get hot from time to time when
governor is set to performance, but the thing is well ventilated and not
too noisy.

The Quadro GPU I have is, performance-wise, equivalent to a GTX 1070, so
good enough for Cuda too. Works well in Ubuntu as soon as you get rid of
the nouveau driver.

Also, if you find a 17” laptop too big for you, there are smaller
alternatives in the same line.

Am no Lenovo fan, but I find this machine offers similar or better
performance than that of a gaming laptop, but without all the flashy skully
flamboyances.

Best wishes,
Jon

On Wed, 13 Feb 2019 at 14:09, Domen Zafred  wrote:

> Dear CCP4 community,
>
> My 8-year-old Dell Inspiron with Ubuntu 10 is waving goodbye and I would
> ask for some help on this bulletin as buying/setting up a new workstation
> is common trouble in our community.
> Has anyone recently set up a laptop for structural work with all the MD,
> docking, etc. *in silico *simulations? Is dual boot Linux/Win still a
> thing, or do you rather run Windows virtually (which VM software)? Are
> there any known issues with Nvidia Quadro P series GPUs or Intel i5&i7
> processors? Has anyone tried Xeon/ECC on a laptop? Does Ubuntu LTS still
> win the hearts of non-enthusiasts?
>
> Any successfully tested laptops (with names and/or listed configuration)
> are very welcome and any answers that include fruits or hamburger names may
> be kindly avoided :)
>
> Thanks a lot,
>
> Domen
>
>
>
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
>
-- 
Dr Jon Agirre
Royal Society University Research Fellow
York Structural Biology Laboratory / Department of Chemistry
University of York, Heslington, YO10 5DD, York, UK
http://www.york.ac.uk/chemistry/research/ysbl/people/staff/jagirre/
Office: /B/K/065 Phone: +44 (0) 1904 32 8252
Twitter: @alwaysonthejazz



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Re: [ccp4bb] 2019 mid-range & high-end LINUX laptops for structural biology

[Tristan Croll]

I've been really happy with the performance of my laptop (a 2016-era 
Asus ROG Strix GL502vs) for MD work. It's a gaming model (GTX 1070 
rather than Quadro, and an i7 CPU), but it's solid and seriously 
powerful. I dual-boot Fedora and Windows (I initially tried with Ubuntu, 
but its setup crashed in the middle of rearranging the hard drive 
partitions leaving me too scared to try again). It works nicely enough, 
other than the occasional issue with kernel updates breaking the Nvidia 
driver and/or GRUB.


On 2019-02-13 14:09, Domen Zafred wrote:

Dear CCP4 community,

My 8-year-old Dell Inspiron with Ubuntu 10 is waving goodbye and I
would ask for some help on this bulletin as buying/setting up a new
workstation is common trouble in our community.

Has anyone recently set up a laptop for structural work with all the
MD, docking, etc. _in silico _simulations? Is dual boot Linux/Win
still a thing, or do you rather run Windows virtually (which VM
software)? Are there any known issues with Nvidia Quadro P series GPUs
or Intel i5&i7 processors? Has anyone tried Xeon/ECC on a laptop? Does
Ubuntu LTS still win the hearts of non-enthusiasts?

Any successfully tested laptops (with names and/or listed
configuration) are very welcome and any answers that include fruits or
hamburger names may be kindly avoided :)

Thanks a lot,

Domen

-

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 https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 [1]

Links:
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[ccp4bb] 2019 mid-range & high-end LINUX laptops for structural biology

[Domen Zafred]

Dear CCP4 community,

My 8-year-old Dell Inspiron with Ubuntu 10 is waving goodbye and I would
ask for some help on this bulletin as buying/setting up a new workstation
is common trouble in our community.
Has anyone recently set up a laptop for structural work with all the MD,
docking, etc. *in silico *simulations? Is dual boot Linux/Win still a
thing, or do you rather run Windows virtually (which VM software)? Are
there any known issues with Nvidia Quadro P series GPUs or Intel i5&i7
processors? Has anyone tried Xeon/ECC on a laptop? Does Ubuntu LTS still
win the hearts of non-enthusiasts?

Any successfully tested laptops (with names and/or listed configuration)
are very welcome and any answers that include fruits or hamburger names may
be kindly avoided :)

Thanks a lot,

Domen



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[ccp4bb] Can anyone run refmac from the WINDOWS command prompt?

[Kevin Cowtan]

Hi! Can anyone run refmac from the WINDOWS command prompt?

I've been experimenting with various combinations of environment variables,
but I always get an error. The one instance of this error on google is an
unanswered CCP4BB question.

Dictionary path has not been defined
Check the environment variable CLIBD_MON
Current value of CLIBD_MON is C:\Apps\CCP4-7\7.0\lib\data\monomers
It should be set to wherever_dict/dic/
===> Error: Wrong path for the dictionary files

 Refmac:  Wrong path for the dictionary files
 Refmac:  Wrong path for the dictionary files

The directory C:\Apps\CCP4-7\7.0\lib\data\monomers contains the following:

 Directory of C:\Apps\CCP4-7\7.0\lib\data\monomers

12/02/2019  15:42  .
12/02/2019  15:42  ..
12/02/2019  15:38  0
12/02/2019  15:39  1
12/02/2019  15:39  2
12/02/2019  15:39  3
12/02/2019  15:39  4
12/02/2019  15:39  5
12/02/2019  15:39  6
12/02/2019  15:39  7
12/02/2019  15:40  8
12/02/2019  15:40  9
12/02/2019  15:40  a
12/02/2019  15:40  b
12/02/2019  15:40  c
28/11/2016  21:43 7,640 COPYING
12/02/2019  15:40  d
28/11/2016  21:43 3,553 dnarna_basepairs.txt
28/11/2016  21:43 3,553 dnarna_basepairs_2.txt
28/11/2016  21:43 5,248 dnarna_params.txt
12/02/2019  15:40  e
27/04/2018  10:59   105,193 ener_lib.cif
28/11/2016  23:06   105,136 ener_one_lib.cif
12/02/2019  15:40  f
12/02/2019  15:40  g
12/02/2019  15:41  h
12/02/2019  15:41  i
12/02/2019  15:41  j
12/02/2019  15:41  k
12/02/2019  15:41  l
12/02/2019  15:41  list
12/02/2019  15:41  m
28/11/2016  21:43 1,372,146 mon_lib_ind.cif
28/11/2016  21:43 6,490,087 mon_lib_ind2.cif
28/11/2016  23:06   120 mon_lib_one_ind.cif
28/11/2016  23:06   396 mon_lib_one_ind2.cif
12/02/2019  15:41  n
31/08/2018  15:5235,312 new_entries.txt
31/08/2018  15:5223,685 not_replaced_entries.txt
12/02/2019  15:41  o
12/02/2019  15:41  p
28/11/2016  21:43 2,490,954 pdb_alt_names.txt
28/11/2016  21:43 7,794 pdb_v2to3.py
12/02/2019  15:41  q
12/02/2019  15:41  r
31/08/2018  15:5229,731 replaced_entries.txt
31/08/2018  15:52   754 revert_list.txt
12/02/2019  15:41  s
12/02/2019  15:42  t
28/11/2016  21:43   557 template_link.pdb
12/02/2019  15:42  u
12/02/2019  15:42  v
12/02/2019  15:42  w
31/08/2018  15:5224 windows_reserved_words.txt
12/02/2019  15:42  x
12/02/2019  15:42  y
12/02/2019  15:42  z
-- 
Professor Kevin Cowtan
York Structural Biology Laboratory / Department of Chemistry
University of York, Heslington, York, YO10 5DD, UK
https://www.york.ac.uk/chemistry/staff/academic/a-c/kcowtan/

EMAIL DISCLAIMER: http://www.york.ac.uk/docs/disclaimer/email.htm



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[ccp4bb] CCP-EM Madrid and Icknield High Resolution EM Model Building & Validation Workshops

[Tom Burnley - UKRI STFC]

Dear all,

There are still some places left for our two model building workshops.  Please 
note deadline for applications is tomorrow (14th Feb).

(1) Introduction to model building and refinement using cryo-EM maps.
14-15 March CNIO, Madrid

Introduction to atomic model building using EM data.  This 2 day course with 
provide lectures and tutorials covering the basics of atomic modeling with 
CCP-EM, Coot, Refmac, LocScale, Coot & Scipion.

Speakers/tutors include:
Tom Burnley (STFC), Jose María Carazo (CNB-CSIC), Ana Casañal (MRC-LMB), Paul 
Emsley (MRC-LMB), Wojtek Galej (EMBL), Arjen Jakobi (TU Delft), Rob Nichols 
(MRC-LMB), Colin Palmer (STFC).

Full details and registration:
http://www.cvent.com/d/p6qmqf

(2) Icknield Model Building Workshop
15-18 April RAL/Diamond Light Source, Oxfordshire, UK

This is a comprehensive course for EM model building covering advanced use of 
ARP/wARP Buccaneer, CCP-EM, Coot, FlexEM, ISOLDE, LocScale, MolProbity, Refmac. 
 The course is designed such that applicants bring their own 'live' high 
resolution datasets to work on with the developers during the workshop.

Tutors and topics include:

Tristan Croll (Cambridge)
ISOLDE: Interactive model building/real-space refinement based on interactive 
Molecular Dynamics

Grzegorz Chojnowski (Lamzin group, EMBL)
ARP/wARP: Automated model building of protein structures

Kevin Cowtan (York)
Buccaneer: Automated model building

Paul Emsley (MRC-LMB Cambridge)
Judit Debreczeni (AstraZeneca)
Coot: Macromolecular model building, model completion and validation

Arjen Jakobi (TU Delft)
LocScale: reference-based density scaling for local sharpening of cryo-EM maps

Jane Richardson (Duke)
MolProbity: All-atom structure validation

Garib Murshudov (MRC-LMB Cambridge)
Rangana Warshamanage (MRC-LMB)
Refmac: Refinement of high-resolution EM structures

Osman Salih (EBI)
EMBD: Deposition in EMBD

Agnel Joseph (CCP-EM, STFC) & Sony Malhotra (Topf Group, Birkbeck)
FlexEM: Fitting and refinement of atomic structures guided by cryoEM density

Full details and registration:
http://www.cvent.com/d/46qmq3

Please apply to course you think most relevant to you (email 
tom.burn...@stfc.ac.uk if unsure).  Each course has a maximum of 40 places to 
register interest.  We will select the most suitable 20 for each course and the 
course fees will be requested if your application is accepted.

Registration for each workshop will close either when all registration places 
are taken or by 14th Feb.  Successful applications will be informed shortly 
afterwards.  Any project details provided will be treated with strict 
confidence.

All the best,

Tom, Colin and Rafa




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