Re: [ccp4bb] Refmac in i2

[Jonathan Cooper]

 The numbers do make sense now: AaA, AbA, etc, correspond to different HETATM 
groups and (what was confusing me a lot) the No. atoms includes riding 
hydrogens. 
On Thursday, 2 May 2019, 23:27:49 BST, Jonathan Cooper 
 wrote:  
 
 In the output statistics part of the GUI there is table of mean B-factors, e.g.
Chain mean B (No. atoms) AAA 23.6( 2596 )AaA 35.4( 29 )AbA 35.2( 10 )AcA 18.9( 
10 )AdA 58.5( 10 )AeA 39.5( 119 )BBB 25.5( 2545 )BaB 42.0( 10 )BbB 46.9( 5 )BcB 
37.3( 92 )
There is an A- and a B-chain in the structure, each with their own waters, but 
can someone explain the AaA, AbA,...AeA, BaB...BcB  notation please, since it 
is not obvious how this relates to the contents of the PDB file. Thank you.

  



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[ccp4bb] Postdoctoral Position on Membrane Protein Structural Biology at Karolinska Institutet, Sweden

[Madhuranayaki Thulasingam]

Dear bulletin board,


A postdoctoral position is available at the Department of Medical
Biochemistry and Biophysics (MBB), Division of Chemistry 2,  Karolinska
Institute, Stockholm. The project is focused on overexpression,
purification, and crystallization of human integral membrane enzymes
involved in the generation of lipid mediators of inflammation, all of which
are drug targets.


MBB is one of the largest departments at Karolinska Institutet equipped
with a range of state-of-the-art technologies in basic research, in
particular, structural biology, proteomics, imaging, and animal models. It
hosts world-leading groups in areas such as molecular neurobiology, matrix
biology, vascular biology, redox regulation, and lipid mediators of
inflammation, which together create a vibrant research environment.


A prospective candidate should hold a recently obtained PhD in structural
biology, biochemistry or a related area and a strong background in protein
chemistry, crystallization and structure determination by X-ray
crystallography and/or Cryo-EM, specifically membrane proteins. The
position is initially available for 2 years with a possibility of extension.



Interested candidates should send a letter of intention and CV including
references as a single PDF file by email to jesper.haeggst...@ki.se and

 madhuranayaki.thulasin...@ki.se. The position will remain open until
filled.


Best wishes,

Madhu.


Madhuranayaki Thulasingam, PhD
Division of Chemistry II
Department of Medical Biochemistry and Biophysics
Karolinska Institute
Biomedicum 9A
Solnavägen 17165, Solna
Stockholm, Sweden.



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[ccp4bb] Home/EU PhD studentship at Leicester

[Peter Moody]

I have funding to cover the stipend and fees for up to 4 years for a PhD
student, funded by the Royal Society.

I should be most grateful if you could tell your brightest and best
students. I am particularly keen recruit someone who wants to explore new
(and not so new)  and exciting ways to look at the way enzymes work.
Imagination and curiosity will be needed.

The link is on FindAPhD
https://www.findaphd.com/phds/project/enzyme-mechanism-through-advanced-techniques/?p109342


Please don't reply to this email address, use peter.moody at le.ac.uk

Peter Moody
Leicester Institute for Structural and Chemical Biology



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[ccp4bb] Last chance to apply: Workshop "Diffraction Data Collection Using Synchrotron Radiation"

[Manfred S. Weiss]

Dear all,

the application deadline for this popular workshop has been
extended to May 08.

Cheers, Manfred



I would hereby like to announce the 6th edition of the workshop
"Diffraction Data Collection Using Synchrotron Radiation", which
will take place from July 04-06, 2019 at the BESSY II storage
ring of the Helmholtz-Zentrum Berlin for Materials and Energy
(HZB) in Berlin.

This workshop is the successor to five previous successful work-
shops, which took place in 2007, 2009, 2011, 2013 and 2016. It is
sponsored by the German Society for Crystallography (DGK) and
organised in cooperation with the DGK Working Group 1 (Biological
Structures).

The workshop comprises a series of basic lectures on the topic
and two extended practical sessions. It is aimed at PhD students
in Biological Crystallography with little or no experience in
diffraction data collection at a synchrotron. The practical sessions
will take place at the MX beamlines located at the electron storage
ring BESSY II.

The workshop fee is 100 EUR for DGK-members and 120 EUR for non-members.
This fee covers all conference material as well as board and lodging
for two nights on the campus in Berlin-Adlershof.

For more information please visit the web page
https://www.helmholtz-berlin.de/events/dgk/index_en.html

The application page is now open. Since the number of students will
have to be limited to 20 in order to ensure that the practical sessions
run efficiently, we expect that the workshop will fill up quickly.
Participants are also expected to present a poster on their own work.
As previously, the best poster will be awarded with an attractive prize.
##

Manfred Weiss

--
Dr. Manfred S. Weiss
Macromolecular Crystallography
Helmholtz-Zentrum Berlin
Albert-Einstein-Str. 15
D-12489 Berlin
Germany



Helmholtz-Zentrum Berlin für Materialien und Energie GmbH

Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V.

Aufsichtsrat: Vorsitzender Dr. Karl Eugen Huthmacher, stv. Vorsitzende Dr. 
Jutta Koch-Unterseher
Geschäftsführung: Prof. Dr. Bernd Rech (kommissarisch), Thomas Frederking

Sitz Berlin, AG Charlottenburg, 89 HRB 5583

Postadresse:
Hahn-Meitner-Platz 1
D-14109 Berlin



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[ccp4bb] 3 years Research assistant position at the Free University of Bolzano (Italy)

[Benini Stefano]

Dear job seekers,

A 3 years Research assistant position (1+1+1) is available at the Free 
University of Bolzano to work in my group on the Euregio IPN95 project:

"Siderophore mediated iron uptake in Erwinia amylovora and Aspergillus 
fumigatus. Towards new strategies in plant and human health"

The project is in collaboration with Professor Hubertus Haas, Medical 
University Innsbruck, and Professor Sheref Mansy, University of Trento.

To apply for the position follow the link:

https://www.unibz.it/en/home/position-calls/positions-for-academic-staff/4094-chimica-organica-dr-benini?group=18

for informal enquiries (and scientific questions!) do not hesitate to contact me

some background information on one of the system to study could be found in 
this publication:
https://doi.org/10.1016/j.jsb.2018.02.002

deadline for application is 04/06/2019

have a nice day

Stefano

Stefano Benini, Ph.D. Assistant Professor
Guest editor of "Carbohydrate-Active Enzymes: Structure, Activity and Reaction 
Products"
A special issue of International Journal of Molecular 
Sciences (IF 3.69) (ISSN 1422-0067). This 
special issue belongs to the section "Molecular 
Biophysics". 
Deadline for manuscript submissions: 31 July 2019

https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes
[Banner H_smaller]

https://sbenini.people.unibz.it/

"I don't like anything that's fake and I hate pretenders!"
*
Bioorganic chemistry and Bio-Crystallography laboratory (B2Cl)
Faculty of Science and Technology
Free University of Bolzano
Piazza Università, 5
39100 Bolzano, Italy
Office (room K2.14):  +39 0471 017128
Laboratory (room E.021): +39 0471 017910
Fax: +39 0471 017009
https://sbenini.people.unibz.it/
orcid.org/-0001-6299-888X

"ogni giorno in più è un giorno in meno..."

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