[ccp4bb] Drug Discovery RFP Application Deadline Approaching - Early Applicant Awards Due Monday!

[Nick DeHaan]

Hi CCP4BB Community,

Our Early Applicant Awards deadline (September 30th) is less than a week
away! You can apply online on our website: Atomwise.com/aims-awards/
.

Atomwise's AIMS Awards program provides academic and non-profit researchers
with virtual small-molecule screening for protein targets and compounds of
top predicted hits for physical testing. A few awards, such as the Early
Applicant Awards, also provide in-kind support (with a value of up to $20k)
for the assay work.

*Over* *300 proposals will be selected* for this cycle of the AIMS Awards
program, and hundreds of projects are already underway at institutions
around the world. Our team is incredibly excited to provide this
opportunity to the global research community, and I encourage you, your
colleagues, and your collaborators to apply!

Kind regards,
Nick

Nick DeHaan, MsE
*Partnering Executive*
*Atomwise*  | *Better Medicines Faster*
ᐧ

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Re: [ccp4bb] design specs/tolerances for crystallization rooms?

[Frank Von Delft]

We have the dehumidification for both 4C and 20C.  Yes, drops dry out faster, 
probably;  there are ways of mitigating, but to do it properly, in my opinion 
you need a 
Shifter,
 sold by OLT (*) -- it makes a big difference 
(manuscript due for submission).


[(*) That's a shameless plug to the extent that we developed it, meaning I 
would say that.  But the vendor OLT are selling and developing it on their own, 
and I'm a customer, not a founder.]



On 26/09/2019 12:59, Sergei Strelkov wrote:

Dear Frank and everyone,


Thanks for useful tips. Snow in liquid nitrogen has been annoying us on a 
regular basis in fact. During some synchrotron sessions, a large percentage of 
crystals were covered with snow. We could never fully figure out how to get rid 
of it, although filtering LN2 before filling the vessel for freezing seemed to 
help a bit...


Would installing a dehumidifier in a standard 20C crystallization room (where 
we mount most of our crystals) be a good idea? Any experiences there? I guess 
the downside would be that small crystallization drops would be drying more 
quickly...


Best wishes,

Sergei


Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49 bus 
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab 
pages: 
http://pharm.kuleuven.be/Biocrystallography


From: CCP4 bulletin board  
on behalf of Frank Von Delft 

Sent: Wednesday, September 25, 2019 7:03
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] design specs/tolerances for crystallization rooms?

My colleague Opher Gileadi gave us an excellent tip when we were designing our 
4C harvesting room, over a decade ago:  set it to 7C.  The crystals are 
unlikely to mind, but it's SO much more comfortable to be in for hours.

I seem to remember he mentioned something like a comfort inflection point as 
you approach 4C.

Install low-flow fans.  Fridge people seem to default to installing hurricane 
machines, you have to tell them that a very very small flow is enough.

Get strong light - probably even those daylight things (we don't have them).  
Being cold is miserable enough already, there's no need to compound it with 
weak light.

Vibration - that dwindles to insignificance if the air flow goes down.

Humidity - we installed (at considerable expense) a low humidity air supply - 
really hard to know just how much it helps, but a few years ago when I had it 
turned it off to help save energy, very quickly I heard complaints about snow 
in the liquid nitrogen becoming a major hassle.  So based on that set of 
anecdotes, I conclude it probably is worth having dry air.

It's much cheaper though if they can design it into the building's 
infrastructure, if it's a new building;  retrofitting turned out to be super 
expensive (in our case).

As dry as possible.  Look at and understand the psychrometric chart (google 
it):  if you're in even vaguely warm or temperate regions (or seasons), cooling 
the intake air to 4C brings it to below dew point, and then condensation and 
snow are guaranteed.

Size - make it as big as you can get away with, with lots of bench and shelf 
space.  Your students will already be miserably cold, no need for them to be 
cramped too.

Good luck!
Frank





On 24/09/2019 23:40, Scott, Emily wrote:
Anyone out there specifically design rooms for (protein) crystallization at ~22 
deg and 4 deg C?  If you have successes or failures and can share any design 
specs with regard to vibration, temperature, and humidity tolerances, it would 
be much appreciated to pass on to the architects for our new laboratory.

Sincerely,
Emily Scott

--
Emily Scott, Ph.D.
Professor, Medicinal Chemistry/Pharmacology/Biophysics
Faculty Director, BioNMR Core Lab
University of Michigan
428 Church Street
Ann Arbor, MI 48109-1065
Phone:  734-764-3530
https://pharmacy.umich.edu/people/scottee
Lab webpage:  http://scottlab.info



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Re: [ccp4bb] design specs/tolerances for crystallization rooms?

[Sergei Strelkov]

Dear Frank and everyone,


Thanks for useful tips. Snow in liquid nitrogen has been annoying us on a 
regular basis in fact. During some synchrotron sessions, a large percentage of 
crystals were covered with snow. We could never fully figure out how to get rid 
of it, although filtering LN2 before filling the vessel for freezing seemed to 
help a bit...


Would installing a dehumidifier in a standard 20C crystallization room (where 
we mount most of our crystals) be a good idea? Any experiences there? I guess 
the downside would be that small crystallization drops would be drying more 
quickly...


Best wishes,

Sergei


Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of 
Pharmaceutical Sciences, KU Leuven O, Campus Gasthuisberg, Herestraat 49 bus 
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab 
pages: 
http://pharm.kuleuven.be/Biocrystallography


From: CCP4 bulletin board  on behalf of Frank Von Delft 

Sent: Wednesday, September 25, 2019 7:03
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] design specs/tolerances for crystallization rooms?

My colleague Opher Gileadi gave us an excellent tip when we were designing our 
4C harvesting room, over a decade ago:  set it to 7C.  The crystals are 
unlikely to mind, but it's SO much more comfortable to be in for hours.

I seem to remember he mentioned something like a comfort inflection point as 
you approach 4C.

Install low-flow fans.  Fridge people seem to default to installing hurricane 
machines, you have to tell them that a very very small flow is enough.

Get strong light - probably even those daylight things (we don't have them).  
Being cold is miserable enough already, there's no need to compound it with 
weak light.

Vibration - that dwindles to insignificance if the air flow goes down.

Humidity - we installed (at considerable expense) a low humidity air supply - 
really hard to know just how much it helps, but a few years ago when I had it 
turned it off to help save energy, very quickly I heard complaints about snow 
in the liquid nitrogen becoming a major hassle.  So based on that set of 
anecdotes, I conclude it probably is worth having dry air.

It's much cheaper though if they can design it into the building's 
infrastructure, if it's a new building;  retrofitting turned out to be super 
expensive (in our case).

As dry as possible.  Look at and understand the psychrometric chart (google 
it):  if you're in even vaguely warm or temperate regions (or seasons), cooling 
the intake air to 4C brings it to below dew point, and then condensation and 
snow are guaranteed.

Size - make it as big as you can get away with, with lots of bench and shelf 
space.  Your students will already be miserably cold, no need for them to be 
cramped too.

Good luck!
Frank





On 24/09/2019 23:40, Scott, Emily wrote:
Anyone out there specifically design rooms for (protein) crystallization at ~22 
deg and 4 deg C?  If you have successes or failures and can share any design 
specs with regard to vibration, temperature, and humidity tolerances, it would 
be much appreciated to pass on to the architects for our new laboratory.

Sincerely,
Emily Scott

--
Emily Scott, Ph.D.
Professor, Medicinal Chemistry/Pharmacology/Biophysics
Faculty Director, BioNMR Core Lab
University of Michigan
428 Church Street
Ann Arbor, MI 48109-1065
Phone:  734-764-3530
https://pharmacy.umich.edu/people/scottee
Lab webpage:  http://scottlab.info



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[ccp4bb] Research Scientist Position, Structural Biology at C4 Therapeutics in Watertown, MA USA

[Joe Patel]

Dear all,

We are looking for additional members to join and support our structural 
biology efforts here at C4 Therapeutics.  If you are (or soon will be) a recent 
graduate with a solid foundation in structural sciences and wish to be a part 
of an emerging new paradigm in drug discovery, please consider applying for the 
role using the link below.

https://easyapply.co/a/6fa7d973-53ef-443b-9e38-1b63b2968dad

Regards,

Joe Patel


Joe Patel, Ph.D.
Director, Biochemistry,
Biophysics & Crystallography

[C42]
490 Arsenal Way, Suite 200
Watertown, MA, 02472
www.c4therapeutics.com







C4 Therapeutics, Inc. Confidentiality Notice: This message is private and may 
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