[ccp4bb] Postdoc Opportunities in Structural Virology at UNSW

[David Jacques]

3 Postdoctoral Position in Sydney, Australia:

Structural Biology of HIV:host interactions and capsid uncoating

We are seeking a 3 exceptional candidates for a postdoctoral positions in, 
protein crystallography, cryo-electron microscopy, and virology to better 
understand the how the HIV capsid responds to host molecules during the process 
of infection. We aim to apply a range of structural and virological methods to 
image model capsid systems and authentic viral capsids in the presence of 
externally applied recombinant cofactors and metabolites in order to understand 
the nature and regulation of the uncoating event. The data will build on our 
recently developed single molecule fluorescence methods for measuring HIV core 
stability and help complete our understanding of the multiple essential roles 
played by the capsid.

The successful candidates will join our team of protein scientists, 
biophysicists, and virologists at the EMBL Australia Node in Single Molecule 
Science, University of New South Wales, Sydney, Australia 
(http://sms.unsw.edu.au) and the ARC Centre of Excellence in Advanced Molecular 
Imaging (http://www.imagingcoe.org). The postdoctoral researchers will have an 
active role in conceiving and implementing approaches, training and supervision 
of research staff and students and presentation of the results in the form of 
publications, international conference presentations and potentially grant 
applications.

The postdoctoral positions are a full-time fixed term position available for 12 
months from January 2020 onwards with strong prospects for a 2-year extension. 
Salary range is A$95,449-A$102,091 per year.

For queries, please contact Dr David Jacques at 
d.jacq...@unsw.edu.au, or apply online:

https://external-careers.jobs.unsw.edu.au/cw/en/job/498123/postdoctoral-fellows-x-2-cryoelectron-microscopy-protein-crystallography

https://external-careers.jobs.unsw.edu.au/cw/en/job/498122/postdoctoral-fellow-virology


Dr David Jacques
Scientia Fellow, Single Molecule Science

Level 3, Lowy Cancer Research Centre
UNSW Sydney, NSW, 2052, Australia

T: +61 (2) 9385 8109
E: d.jacq...@unsw.edu.au
W: https://sms.unsw.edu.au/david-jacques

CRICOS Provider Code 00098G

[cid:image001.png@01D38DFE.59C971A0]




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Re: [ccp4bb] D amino acids in Coot

[Jonathan Cooper]

 Hello, you are right but you can get round it by reading in a cif for D-ala 
first i.e. 'Import cif dictionary' before doing the 'Get Monomer' thing. I got 
the cif file from the web (its attached). If you do this in the right order you 
do get a D-ala on the screen!
On Wednesday, 30 October 2019, 18:38:56 GMT, Pavel Mader 
 wrote:  
 
 Hi everyone,
if I load D-Ala (Get monomer DAL) in Coot and superpose (LSQ) it with regular 
L-Ala (ALA), the two amino acids look identical to me (not mirror images of 
each other), see attached image. I have the same problem with D-His. Is this 
normal, or am I missing something?
Thanks,
Pavel

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Component_data_DAL.cif
Description: Binary data

[ccp4bb] Cryo-EM/X-ray/NMR Postdoctoral Position

[Rajesh Ramachandran]

*POSTDOCTORAL POSITION AVAILABLE *

*STRUCTURAL BIOLOGY OF MEMBRANE REMODELING*



A NIH-funded postdoctoral position is available immediately in the
Ramachandran Lab at Case Western Reserve University (CWRU) to study the
structural aspects of protein-mediated membrane remodeling during endocytic
and mitochondrial membrane fission. The position requires a Ph.D. in
biochemistry or biophysics with a strong focus on structural biology (X-ray
crystallography, NMR, or cryo-EM) and membrane biophysics. This position
will provide an excellent opportunity to learn and apply a wide array of
structural and biophysical techniques to explore protein function in and on
membranes. The Ramachandran laboratory also employs a host of cutting-edge
spectroscopic approaches including FRET, fluorescence correlation
spectroscopy (FCS) and fluorescence lifetime imaging (FLIM) to explore
protein-protein and protein-membrane interactions in membrane remodeling
and fission, both *in vitro* and *in vivo*. The Ramachandran lab and the
facilities at CWRU are equipped with state-of-the-art instrumentation for
structural biology, including a newly acquired 300 kV Titan Krios G3i
microscope coupled to a Gatan K3 direct detection camera for
high-resolution cryo-EM.

*Requirements*: Applicants must be highly motivated and must have
demonstrated experience (i.e. relevant publications) in protein
biochemistry and structural biology. The candidate should have a strong
conceptual and experimental background in biochemistry and biophysics, as
well as in the mechanistic dissection of structure-function relationships
in proteins; he/she should be independent, proactive, hardworking and
productive; only candidates that have first-author publications (or
articles in press) will be considered. Candidates must have completed their
PhD at the time of appointment. Salary will commensurate with experience
and will adhere to current NIH guidelines. Interested candidates should
submit their CV, reprints of selected publications, three reference letters
(directly from referees), and a cover letter summarizing their experience,
long-term goals, and estimated start date directly to Rajesh Ramachandran
at rxr...@case.edu.

*Relevant Publications. *Please visit our webpage at
*https://physiology.case.edu/people/faculty/rajesh-ramachandran/*


-- 
*Rajesh Ramachandran, Ph.D.,*
*Associate Professor of **Physiology & Biophysics,*
*School of Medicine, *
*Case Western Reserve University,*
*Cleveland, OH 44106-4970, USA*
*Tel: 1-216-368-2513*



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[ccp4bb] D amino acids in Coot

[Pavel Mader]

Hi everyone,



if I load D-Ala (Get monomer DAL) in Coot and superpose (LSQ) it with
regular L-Ala (ALA), the two amino acids look identical to me (not mirror 
images of each other), see attached image. I have the same problem with D-
His. Is this normal, or am I missing something?




Thanks,




Pavel



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[ccp4bb] Reminder - last change to apply for a travel bursary to CCP4 Study Weekend 2020 - closes tomorrow!

[Karen McIntyre - UKRI STFC]

Tomorrow is the last chance for students and young post-docs at non-UK 
institutions to apply for a travel bursary towards the cost of attending the 
CCP4 Study Weekend.



More details can be found at 
http://www.cvent.com/events/ccp4-study-weekend-2020/custom-17-001c74f5ecfa4987af3e908c7a9d7d60.aspx?dvce=1



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