[ccp4bb] ACA hotel room block closing June 17

2022-06-14 Thread Anna Gardberg 
Dear Colleagues,

The ACA annual meeting in Portland is fast approaching. The meeting will be
held from July 29 - August 3, 2022. The hotel room block at the Marriott
Downtown Waterfront will be closing on June 17th. Book your room today
!  Register for
the meeting  and consider
taking one of the  workshops  on July
29.



WK1: Applications of Small Angle Scattering to Structural Biology: An
Introduction

WK2: Crystallographic and cryo-EM Structure Solution with Phenix

WK3: Cryo-Electron Tomography: State of the Art Methods

WK4: Hands-on Single-Particle Cryo-EM Data Analysis with Cryo EDU

WK5: CryoEM Sample Preparation Training using Center Merit Badges

WK6: Advanced Software Tools for Single Crystal Diffraction

WK7: Visualizing Structure

WK8: Microcrystal Electron Diffraction (MicroED)



If you are already registered for the proximally located M&M conference, you
can register for the ACA workshops and/or an ACA one day pass

.



We look forward to seeing you in Portland!



The 2022 Annual Meeting Committee



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Re: [ccp4bb] X-ray crystallography defense questions

2022-06-14 Thread Nukri Sanishvili 
Hi Jessica,
Your subject being membrane proteins, I might ask a question if your
conclusions and claims are commensurate with the data quality but as
Eleanor says, it really depends on what you are actually claiming and what
kind of data you have.
Cheers,
Nukri

On Tue, Jun 14, 2022 at 10:59 AM Jessica Besaw  wrote:

> Hello CCP4!
>
> I am preparing for my PhD defense on X-ray crystallography of membrane
> proteins.
>
> So my ask from you folks is: *What X-ray crystallography questions would
> you ask in a PhD defense? *
>
> All questions - easy, tough, tricky - are welcome.
>
> Thank you kindly,
>
> Jessica Besaw
>
>
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>



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[ccp4bb] Problem with restraint library in coot 0.9.8.3 (issue with ccp4 8.0?)

2022-06-14 Thread Zhen Gong 
Hi,

I had problems with restraint library in coot 0.9.8.1 when I updated to CCP4 
8.0. I could not do any real space refinements because of this problem. 
Yesterday, I updated to CCP4 8.0.002 and coot was updated to 0.9.8.3EL. The 
problem in restraint library was still not solved. Will re-installation of ccp4 
be able to solve the problem? Many thanks in advance!

Best,
Zhen



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Re: [ccp4bb] X-ray crystallography defense questions

2022-06-14 Thread Eleanor Dodson 
Partly depends on what you have written! Faux pas would lead to a
question.. but I would probably concentrate on what the structure has told
you about your protein system. I would expect you to know a bit about the
strengths and limitations of the technique.
Eleanor

On Tue, 14 Jun 2022 at 16:58, Jessica Besaw  wrote:

> Hello CCP4!
>
> I am preparing for my PhD defense on X-ray crystallography of membrane
> proteins.
>
> So my ask from you folks is: *What X-ray crystallography questions would
> you ask in a PhD defense? *
>
> All questions - easy, tough, tricky - are welcome.
>
> Thank you kindly,
>
> Jessica Besaw
>
>
>
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>



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Re: [ccp4bb] X-ray crystallography defense questions

2022-06-14 Thread Klaus Futterer 
Q1: who needs X-ray crystallography - we have cryoEM?


Q2: what's the phase problem and is it still a problem (after so many years)?


;-)




===
Klaus Fütterer, PhD
Reader in Structural Biology


School of Biosciences
LES CollegeEmail: 
k.futte...@bham.ac.uk
University of Birmingham Phone: +44 - 121 - 414 5895
Birmingham, B15 2TT, UK(voice mail messages will 
forward to my email inbox)

My normal working hours are Mon - Fri 8.30 - 5.30 pm.


===

From: CCP4 bulletin board  on behalf of 
jbesaw1...@gmail.com 
Sent: 14 June 2022 16:58:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] X-ray crystallography defense questions

Hello CCP4!

I am preparing for my PhD defense on X-ray crystallography of membrane proteins.

So my ask from you folks is: What X-ray crystallography questions would you ask 
in a PhD defense?

All questions - easy, tough, tricky - are welcome.

Thank you kindly,

Jessica Besaw






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[ccp4bb] X-ray crystallography defense questions

2022-06-14 Thread Jessica Besaw 
Hello CCP4!

I am preparing for my PhD defense on X-ray crystallography of membrane
proteins.

So my ask from you folks is: *What X-ray crystallography questions would
you ask in a PhD defense? *

All questions - easy, tough, tricky - are welcome.

Thank you kindly,

Jessica Besaw



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[ccp4bb] Postdoctoral position available in Bethesda, MD, US

2022-06-14 Thread Pazgier, Marzena 
A postdoctoral position is available immediately to participate in an
interdisciplinary research program in structural immunology in the
laboratory of Dr. Pazgier (https://www.usuhs.edu/node/68252) at the
Uniformed Services University of the Health Sciences (USU) in Bethesda, MD.
Candidates are expected to work on ongoing research projects in structural
biology with the aim to understand the molecular basis of the adaptive
immune response to HIV-1 and Sars-Cov-2 and the ultimate goal to develop
new protein-based therapeutics and novel vaccine concepts. Strong research
interests and experience in molecular cloning, protein expression,
molecular interaction analysis, X-ray crystallography and/or Cryo-EM, as
well as outstanding interpersonal, oral, and written communication skills,
are required for this post. Candidates with an immunological background are
invited to apply.


Candidates need to be highly motivated individuals who enjoy working as
part of a dynamic, collaborative and multidisciplinary team. Preference
will be given to candidates with exceptionally strong experimental skills,
a strong record of peer-reviewed publications, and a demonstrated
commitment to basic and translational research. Qualified candidates should
have a doctorate in chemistry/biochemistry, microbiology, immunology or a
closely-related field. Candidates at any career stage are invited.


USU is in Bethesda, MD directly across the street from the NIH, and is
therefore part of an extraordinarily vibrant local biomedical research
community. Our research projects take advantage of recently established
Core facilities for Nextgen sequencing, Bioinformatics and Structural
Biology analyses. USU has a strongly collaborative environment, with weekly
Immunology data club and journal club meetings, as well as regular research
seminars featuring local and invited speakers.


Candidates should apply to the Henry M. Jackson Foundation:
http://careers.hjf.org submitting a CV plus names and contact information
for 3 references.

Note: you may also directly contact Dr. Pazgier directly,
marzena.pazg...@usuhs.edu.

--



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