[ccp4bb] Post-doctoral Position in Structural Biology and Membrane Trafficking: CIC bioGUNE, Bilbao, Spain
The position is available in the laboratory of Aitor Hierro to work in the area of *structural biology* and *membrane trafficking*. We study the interactions, in molecular mechanistic detail, that underlie the selectivity of cargo transport vesicles for recognizing the correct target membrane. We do this using a combination of structural, biochemical and biophysical approaches. The focus of the project is on a series of soluble and membrane associated proteins (and their complexes), which coordinate tethering and fusion of transport vesicles from endosomes to the Golgi apparatus (for references, see PMDIs: 20615984; 21183348 and 17891154). The position requires PhD. degree with a strong background in molecular biology for heterologous recombinant protein expression in different hosts such Escherichia coli, insect cell-baculovirus and mammalian cells. Previous experience in X-ray crystallography and the use of biophysical or biochemical techniques for the characterization of protein complexes would be an advantage. Local resources at the institute include shared state-of-the-art facilities for high throughput crystallization, and in-house X-ray data collection. We also have regular synchrotron beamtime. Other biophysical instrumentation such DLS, ITC, DSC, CD, SEC-MALLS and fluorescence spectrometers are also available. For testing our structural and *in vitro* analyses we have complementary collaborations with leading laboratories in the cell biology field. The institute is well-situated with numerous groups working in X-ray crystallography, NMR and EM that form a critical mass in the area of structural biology. This position offers an excellent opportunity for an experienced protein biochemist/biophysicist or cell biologist to complement his or her expertise in X-ray crystallography working at the juncture between structural biology and cell biology. The position is available beginning December 2011 and funded for 3+2 years. Applications will be accepted until the position is filled. * Interested candidates* please email a cover letter, your CV, and names (including email address) of at least two referees to *r...@cicbiogune.es* with *ref. 3131*in the subject.
[ccp4bb] Platform Manager at CIC-bioGUNE (Bilbao, Spain)
A position of Manager for Macromolecular Crystallography Platform is immediately available at the Structural Biology Department of CIC bioGUNE (Bilbao, Spain). The platform includes a Bruker Microstar-H rotating anode generator, MAR345-DTB image plate, Bruker-135 mm CCD detector system, Helios confocal optics, two Oxford Cryosystems Series 700, TECAN-EVO100 liquid handling robot, Mosquito nanodispenser, a Bruker Crystal Farm, and supporting computers/software. A competitive salary will be offered. Responsibilities: Routine maintenance, operation and development of the Platform Training and supervising users Key Qualifications: BSc or higher degree in physics, chemistry, biochemistry, biophysics or related field. Working experience in the area of macromolecular crystallography, instrumentation, computer controls: Experience with robotics hardware and control systems. Experience with the collection of experimental data for biological crystallography. Experience in the use of computer programs required for biological crystallography. Excellent communication skills. To apply please submit a cover letter, CV, and three letters of reference addressed to [EMAIL PROTECTED] with ref. 8001 in the subject line.
