[ccp4bb] Research Scientist/Postdoc position- Institute of Molecular Medicine Center Translational Cancer Research of UTHealth at Houston
Dear all, Our group (Institute of Molecular Medicine - Center for Translational Cancer Research of UTHealth at Houston) is looking to hire a research scientist/postdoc immediately with experience in protein chemistry, protein engineering, and/or Bioconjugation. Our group is working on developing novel antibody-drug conjugates (ADCs) for effective cancer treatment. For more information, please visit: https://www.uth.edu/imm/faculty/profile?id=0474c506-9d8e-488b-8295-083ec70fb688 The candidate for this position will be highly motivated to lead and perform characterization and development studies on ADCs. This will include protein expression, purification, and conjugation reaction. In addition, the candidate will design and execution of development studies to identify and determine appropriate conditions for producing several types of ADCs. Successful candidates should have a Ph.D. in Biochemistry, Biophysics, Chemistry, Pharmaceutical Science, or a related field. Experience in the production, purification, and characterization of protein on a research scale is required. Demonstrate excellent communication and writing skills. Interested candidates, please send a CV and a brief statement of interest, and contact information for three references to Dr. Qingyun Liu (qingyun@uth.tmc.edu) or Dr. Yukimatsu Toh (yukimatsu@uth.tmc.edu) University of Texas Health Science Center at Houston (UTHealth) Established in 1972 by The University of Texas System Board of Regents, The University of Texas Health Science Center at Houston (UTHealth) is Houston’s Health University and Texas’ resource for health care education, innovation, scientific discovery, and excellence in patient care. The most comprehensive academic health center in the UT System and the U.S. Gulf Coast region, UTHealth is home to schools of biomedical informatics, biomedical sciences, dentistry, nursing, and public health, and the John P. and Kathrine G. McGovern Medical School. UTHealth includes The University of Texas Harris County Psychiatric Center, as well as the growing clinical practices UT Physicians, UT Dentists and UT Health Services. The university’s primary teaching hospitals are Memorial Hermann-Texas Medical Center, Children’s Memorial Hermann Hospital and Harris Health Lyndon B. Johnson Hospital. Yukimatsu Toh, Ph.D. Assistant Professor Center for Translational Cancer Research The Brown Foundation Institute of Molecular Medicine 1825 Pressler St. | Rm 33014 | Houston, TX 77030 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] multi-crystals averaging by using PHENIX 1.4-3 version
Hi all Merry Christmas!! Sorry for not ccp4 question. I have a problem about multi-crystals averaging by using PHENIX 1.4-3 version. I have two crystals. one is low resolution crystal form I(3.6A P6422; monomer) another is crystal form II(3.1A P42212; dimer). I did MAD phasing by using crystal form I and got a good density map. I want to do phase extension from crystal form I to crystal form II for model building(from phase of monomer to dimer). My question is can I use the multi-crystals averaging by the new version PHENIX 1.4-3 program? Is it possible that extend the phase from monomer to multimer? I check the phenix.multi_crystal_average, it seems that only more than one chains of crystal structures can be carried? In my case, phased crystal form I only has one molecule in asymmetry. I also want to know the details of how to use DMMULTI of ccp4i. Any suggestions and advice are welcome. Thak you very much. Jun
[ccp4bb] a refinement problem
Dear all, I have a refinement problem. I used a perfect twinning structure(data set processed with P21212, supected pseudo-symmetry and expanded the data to P2. twnning parameter used the refinement) as a search model to do MR. After MR I can get a Rfac 0.52 structure. I am refining the structureat 2.8A using CNS, however, after many refinement cycles, Rfree is still over 40% . Actually, they are the same protein. the search model is a RNA-protein complex structure, and my structure(spacegroup C2221 )is a apo structure. I can see a good density in my structure. I don't know whether the high Rfree is model bias from twinning structure or not? How to do the high Rfree refinement? Any advice and suggestion are welcome. Thank you very much in advance. Best Jun
Re: [ccp4bb] MR problem --molrep
HI all, Thank you very much for your kindly answer. I also tried to use different program like phaser, amore, and some MR servers. actualy, I don`t know how many molecule in AU clearly. 2-6 molecules in 76%- 35% of solvent content. 4mer is 50% of solvent content. the homolgous structure has high solvent content over 70%. when i used phaser program to do MR, I can get several solutions but not good structure conformation. I don`t know how to use phaser do automatic more than 2 molecules search like molrep program. As spacegroup, I checked the C222 and C2221. the phaser program can give both solutions when I changed the search model or maximum solution limit. my question is how i can search more than 2 molecules by phaser program like molrp only input over 2. by the way, my diffraction data have low completeness(55%) and low redandancy(1.8). Thanks in advance!! carl roja
[ccp4bb] MR problem --molrep
Dear all I tried to solve one structure by ccp4i molrep(resolution at 3.0 A, space group C222, sequence ID 30%). I can get a good Rfactor 0.528 at first translation function. However, the second translation function Rfac is 0.526, the third is 0.525, the fourth is 0.525. All of the translation function Rfacs are too closed. I changed the model and minimum resolution for search, the Rfactor closed no any improved. My structure estmates four molecules in the aymmetric unit. This is my first time found the closed Rfac by molrep. From the low translation function Rfac, it seems that it is correct solution. I checked the solution and found some clashes in the structure.I am not sure why the Rfactor too closed in next the molecule search? I know this is unusual solution by molrep. Does it mean the diffraction data has problem? Any suggestions are welcome.Thank you in advance! Carl soja
Re: [ccp4bb] MR problem --molrep
Dear Debajyoti Thank you very much for your help. I input the each peak values as 10 and carried out the rotation and translation function again. I didn't get a improved solution by molrep. can i edit the self-rotaion function and translation function solution file as input ? best regards, carl soja Hi, I dare to say about the possible way to do molrep from my recent experience. You can choose rotation and translation function job at first and do the self rotation fuction (for multimer) after that.Each of these run will generate two different outputs *_rf.molrep_rf ans *_srf.molrep_rf. Make the third run inputting both of these peak values. Give NMON as 4. You can also use the protein sequence hare. Then refine it with Refmac, that again should decrease your R factor. I will be highly benefitted if some one point out my mistake. regards Debajyoti On Fri, 25 Jul 2008 Carl Soja wrote : Dear all I tried to solve one structure by ccp4i molrep(resolution at 3.0 A, space group C222, sequence ID 30%). I can get a good Rfactor 0.528 at first translation function. However, the second translation function Rfac is 0.526, the third is 0.525, the fourth is 0.525. All of the translation function Rfacs are too closed. I changed the model and minimum resolution for search, the Rfactor closed no any improved. My structure estmates four molecules in the aymmetric unit. This is my first time found the closed Rfac by molrep. From the low translation function Rfac, it seems that it is correct solution. I checked the solution and found some clashes in the structure.I am not sure why the Rfactor too closed in next the molecule search? I know this is unusual solution by molrep. Does it mean the diffraction data has problem? Any suggestions are welcome.Thank you in advance! Carl soja
