Hi Oliver,
Have a look at the viral polyhedra work:
Coulibaly, F., et al., The molecular organization of cypovirus
polyhedra. Nature, 2007. 446(7131): p. 97-101.
Coulibaly, F., et al., The atomic structure of baculovirus
polyhedra reveals the independent emergence of infectious crystals in DNA and
RNA viruses. Proceedings of the National Academy of Sciences of the United
States of America, 2009. 106(52): p. 22205-22210.
Ji, X., et al., How baculovirus polyhedra fit square pegs into
round holes to robustly package viruses. Embo Journal, 2010. 29(2): p. 505-514.
The crystals are typically small; wild type ~5um^3; recombinant ~10um^3.
Because these are readily produced with very consistent unit cell, merging
multiple crystals makes sense and allows best accumulation of completeness and
redundancy. It is possible to collect complete data from a single, large
crystal but with significant radiation damage. These crystals have unusually
low solvent content ~22% so could be regarded as not generally representative.
Cheers,
Danny
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Oliver
Zeldin
Sent: 11 October 2013 01:07
To: ccp4bb
Subject: [ccp4bb] Smallest crystal used for a whole dataset (at a synchrotron!)
Dear All,
I was wondering if anyone has a more up to date reference on the smallest
crystal (fibrils not included!) that has been used to collect a whole dataset?
Also, the smallest crystals used for a multi crystal approach? In both cases,
not including any X-FEL structures.
I'm currently working off the citations in James Holton's 'A beginner's guide
to radiation damage', but am sure that there must be a new record coming from
the microfocus beamlines by now.
Cheers,
Oliver Zeldin
Brunger Lab
Stanford, CA
--
This e-mail and any attachments may contain confidential, copyright and or
privileged material, and are for the use of the intended addressee only. If you
are not the intended addressee or an authorised recipient of the addressee
please notify us of receipt by returning the e-mail and do not use, copy,
retain, distribute or disclose the information in or attached to the e-mail.
Any opinions expressed within this e-mail are those of the individual and not
necessarily of Diamond Light Source Ltd.
Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments
are free from viruses and we cannot accept liability for any damage which you
may sustain as a result of software viruses which may be transmitted in or with
the message.
Diamond Light Source Limited (company no. 4375679). Registered in England and
Wales with its registered office at Diamond House, Harwell Science and
Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
