[ccp4bb] opentrons pipetting robot

[Doug Juers]

Hello All,

I've just learned about the opentrons pipetting robot, which appears to be 
quite affordable relative to other robots. I'm wondering if anyone here on ccp4 
bb has any experience with it - for creating crystallization screens and/or 
setting drops? 

Best,
Doug


-
Douglas Juers
Physics Department
Program in BBMB
Whitman College



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

[Doug Juers]

Hi Yuvraj,

Here are some thoughts in addition to what's been suggested already:

1. A good recent reference is here: 
http://journals.iucr.org/f/issues/2015/06/00/en5564/index.html

2. The image you sent suggests cubic ice, indicating a higher glycerol 
concentration (maybe 35 or 40%) is needed. Also consider stepwise 
equilibrations.

3. Glycerol and ethylene glycol can increase protein solubility, so if the 
crystal cannot tolerate the higher glycerol concentration try some other 
classes of cryoprotective agents (e.g. sugars, methylamines (i.e. TMAO), 
alcohols, mixtures...see the above reference)


Best,
Doug