Hi Afshan,
Have look at http://www.douglas.co.uk/Scaling_Up.htm, Patrick has some
general tips on scaling up. Elsewhere on his site you'll find tips for
conversion to microbatch, you may want to try that out as well. I assume
the screen crystals were not big enough to mount?
Flip
Op 4/16/2013 11:21, Afshan Begum schreef:
Dear All,
I have encountered one problem to optimization the crystallization
condition manually. Actually i got the good shape and even size
crystal in a screening plate. The condition are :
20% PEG 6000, 0.1M MES pH 6.0 whereas protein in 10mM Na-acetate pH
5.7 contain 50mM NaCl, 1mM EDTA &5mM BME.
When I tried to optimize this on Linbro the drops are become heavily
precipitate even a mints. I tried so many things to avoid this ppt
such as start PEG conc from 7%, dilute protein at half conc., add more
salt in a reservoir solution but no succeeds.
The protein prep is same which was using to get the Hits.
I would be very thankful if you people give me nice suggestions.
Thanks
Best Regards
AFSHAN
