[ccp4bb] Two postdoctoral positions at the Walter and Eliza Hall Institute, Melbourne
Dear colleagues, We are seeking two talented and enthusiastic postdocs to join teams within the Inflammation Division of the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia: one focused on structural biology+cell biology; and another postdoc to study protein-protein interactions. 1. A postdoc or senior postdoc to study the structural and cell biology of pseudokinases, with a focus on the necroptosis effector MLKL https://www.wehi.edu.au/research-officer-senior-research-officer-postdoctoral-scientist Enquiries to jam...@wehi.edu.au<mailto:jam...@wehi.edu.au> 2. A postdoc to study the interactions of signaling proteins using biophysics and proteomics. https://www.wehi.edu.au/research-officer-postdoctoral-scientist Enquiries to snichol...@wehi.edu.au<mailto:snichol...@wehi.edu.au> With thanks and best wishes James -- Assoc. Prof. James M. Murphy, PhD Head, Inflammation Division Walter and Eliza Hall Institute of Medical Research 1G Royal Parade Parkville, VIC 3052, Australia T: +61 3 93452407 F: +61 3 93470852 http://www.wehi.edu.au/people/james-murphy ___ The information in this email is confidential and intended solely for the addressee. You must not disclose, forward, print or use it without the permission of the sender. The Walter and Eliza Hall Institute acknowledges the Wurundjeri people of the Kulin Nation as the traditional owners of the land where our campuses are located and the continuing connection to country and community. ___ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] Postdoctoral position in crystallography - Melbourne, Australia
Dear all, An opportunity exists for a highly skilled and experienced Postdoctoral Scientist to join my lab in the Cell Signalling Cell Death Division of the Walter and Eliza Hall Institute of Medical Research in Melbourne, Australia. The laboratory is focused on the cellular signalling functions of a group of proteins related to protein kinases, called pseudokinases, which are believed to lack catalytic activity. The position is particularly interested in understanding their functions as molecular switches and how toggling these switches, whether by endogenous protein interactors or by small molecule modulators, regulates signalling pathways. We have a particular interest in the mechanism underlying activation of the pseudokinase, MLKL, which is a key mediator of cell death in the recently described necroptosis pathway (Murphy et al., Immunity 2013 39:443; Murphy et al., Biochem J 2014 457:369; Hildebrand et al., PNAS 2014 111:15072). Applicants should possess a doctorate (PhD), with experience in protein crystallography including crystallisation, data collection and structure determination. Additional experience with cell culture techniques, including flow cytometry, immunoprecipitation, immunoblot and/or insect cell expression is also preferred. This position is available for 2 years in the first instance. Salary is dependent on qualifications and experience. Up to 17% superannuation and attractive salary packaging options are available. Applications close on March 20, 2015. Please see http://www.wehi.edu.au/research-officer-murphy-laboratory for further details on the position and application process. Best wishes James Murphy -- James M. Murphy, PhD Cell Signalling and Cell Death Division Walter and Eliza Hall Institute of Medical Research 1G Royal Parade Parkville, VIC 3052, Australia http://www.wehi.edu.au/people/james-murphy Save the date: December 5-8, 2015, San Diego, CA. Kinases and pseudokinases: Spines, scaffolds and molecular switches http://www.asbmb.org/ASBMBMeetings/SpecialSymposia/Kinases/ __ The information in this email is confidential and intended solely for the addressee. You must not disclose, forward, print or use it without the permission of the sender. __
[ccp4bb] Ion identification in crystal structures
What is a good resource for identifying what seem to be ions (Na+, Cl-, CO3-, NH4-) and not simply water molecules in a crystal structure? James W. Murphy, Ph.D. Associate Research Scientist; Dept. of Pharmacology Facility Manager; Macromolecular Crystallography Facility Yale University School of Medicine 333 Cedar Street, SHMB345 New Haven, CT 06510 Office: 203-737-1526 Cell: 203-906-5759 Fax: 203-737-2027
Re: [ccp4bb] SDS-PAGE of peptides
I have had very good results using 10% Tris-tricine gels, following the protocol in Current Protocols for our chemokines (~8,000 mw) James W. Murphy, Ph.D. Associate Research Scientist; Dept. of Pharamcology Facility Manager; Macromolecular Crystallography Facility Yale University School of Medicine 333 Cedar Street, SHMB345 New Haven, CT 06510 Office: 203-737-1526 Cell: 203-906-5759 Fax: 203-737-2027 -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jacob Keller Sent: Thursday, February 11, 2010 2:13 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] SDS-PAGE of peptides Dear Crystallographers, does anybody have any good tricks for getting nice, sharp SDS-PAGE bands of peptides 10kD? Regards, Jacob Keller *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu ***
Re: [ccp4bb] SAD phasing at home source
I have had success with Iodine, soak your crystal in your cryo + Potassium Iodide _ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of james09 pruza Sent: Monday, October 26, 2009 2:33 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] SAD phasing at home source Dear All, I need some suggestions regarding the SAD phasing using home source. What the the most commonly used HA for SAD phasing for Cu anode. All suggestions are welcome. Thanks. James
[ccp4bb] refmac and coot library file creation difficulty
I need to model in tetrachloroaurate molecules into a structure, from our heavy atom soak of Potassium tetrachloroaurate. I created a pdb file by using chemdraw and exporting as an *.sdf and opening in pymol and saving as a *.pdb (attached) I let refmac create a library file (also attached) that coot does not recognize. Also, coot does not draw bonds between the atoms. Also, refmac, even with its own library file, moves the Cl atoms too close and too far from the Au atom. It should be a planar molecule. Is there a better way to create a refmac *.cif file? The dunded prodrg server cannot handle Au atoms. I chose TCU as the monomer name as it does not occur in the refmac monomer libararies. Thanks TCU.pdb Description: Protein Databank data TCU.cif Description: Binary data
