[ccp4bb] OFF TOPIC: recommendations for High Pressure Homogenizers

2012-08-04 Thread Marcelo Carlos Sousa
Sorry for the off topic question, but it is relevant to those that produce 
proteins for crystallographic purposes:

We are looking to replace an old French Press with a high pressure homogenizer 
for cell disruption (E. coli and yeast). We are currently looking at the 
Avestin C3 and the Niro Panda 2000. I would appreciate any feedback (positive 
or negative) from users of these instruments (or any other competing 
homogenizer).

Thanks in advance

Marcelo


[ccp4bb] OFF TOPIC: Aquifex aeolicus DNA

2010-05-06 Thread Marcelo Carlos Sousa
Sorry for the off topic question but since Aquifex aeolicus is a favorite of 
many structural biologists I wonder if anybody can point me to a reliable 
source for DNA from this critter (or any other Aquifex). ATTC does not seem to 
have it...

Thanks in  advance

Marcelo


[ccp4bb] HHMI X-ray lab manager/Research Scientist Job

2007-11-29 Thread Marcelo Carlos Sousa

To all interested parties:

A Research Scientist / X-ray lab manager position in the lab of Tom  
Cech in Boulder, Colorado is open.


Job Description:

The Howard Hughes Medical Institute, a leading biomedical research  
organization, is seeking an experienced scientist to manage our X-ray  
crystallography facility at the Cech research laboratory at the  
University of Colorado campus in Boulder.


The facility is equipped with two Rigaku generators and R-Axis IV++  
detectors for data collection, a linux cluster, and Macintosh  
computers for calculations and graphics, and a Fluidigm microfluidics  
crystallizer. The successful candidate will (1) Carry out routine  
maintenance on these instruments and coordinate service under service  
contracts; (2) perform routine Unix system administration and  
crystallographic software management; (3) provide basic training in  
the use of x-ray crystallographic equipment and software to users;  
and (4) engage in independent or collaborative research.


Preferred qualifications include a Ph.D. degree in an appropriate  
field of science or technology, and three years of experience in a  
Research Specialist level position. Previous experience in Unix/Linux  
system administration and routine maintenance of crystallography  
hardware is essential.


HHMI offers an excellent salary and comprehensive benefits package.  
Interested candidates please forward a statement of interest, CV,  
publications and references by December 30, 2007 to Ms. Robbie  
Haywood Oglesby, Administrative Assistant, 1200 Moursund Ave S101,  
Houston, Texas 77030 or e-mail [EMAIL PROTECTED] (Include Research  
Specialist - Cech Laboratory in the subject line of your e-mail).





Marcelo C. Sousa, Ph.D.
Department of Chemistry and Biochemistry
Cristol Chemistry Building. Room 100
215 UCB
University of Colorado at Boulder
Boulder, CO 80309
Phone: (303) 735 4341
Fax: (303) 492 5894