Dear Tim,
I have used coot.
"merge molecules" (calculate -> merge molecules).
Using coot I have moved the molecules to the same asymmetric unit.
I have the same problem with two different proteins in one case it works.
However, for the other I am still working :-(
Greetings,
Mônika
2014-02-20 11:09 GMT-03:00 Tim Gruene :
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> Dear Monika,
>
> would you mind summarising how you solved your problem? It might help
> people with similar problems!
>
> Regards,
> Tim
>
> On 02/20/2014 02:45 PM, Monika Coronado wrote:
> > Dear users,
> >
> > thanks alot for the tips. The problem was solved!
> >
> > Warmest regard,
> >
> >
> >
> > Monika Coronado
> >
> >
> > 2014-02-20 0:57 GMT-03:00 Jens Kaiser :
> >
> >> Monika, There are several possible causes for the problem you
> >> are encountering, but your description is a little too vague to
> >> discern them. Scenario 1) You ran phaser with the option "all
> >> possible spacegroups", for several different components of your
> >> crystal, setup individually, and the runs do not agree on the
> >> "best spacegroup"? -- In that case, phaser had problems
> >> determining the correct spacegroup, I'd suggest you search for
> >> all components, but in separate runs for each possible
> >> spacegroup. Scenario 2) You assumed your spacegroup assignment
> >> was correct, and ran MR for each of your components individually,
> >> and when you display the solutions, they overlap. In this case,
> >> you might have your solutions on different origins. The best way
> >> out is to use the first solution as a "fixed solution", which is
> >> possible in most MR programs, and then search for the next
> >> component. There might be other scenarios, if you describe your
> >> situation in more detail (how many components in the crystal
> >> setup, what program you used, how you used it, and what you mean
> >> by "different symmetries"), we might be able to help you better,
> >>
> >> Cheers,
> >>
> >> Jens
> >>
> >> On Tue, 2014-02-18 at 14:59 -0300, Monika Coronado wrote:
> >>> Dear,
> >>>
> >>> Does anyone know how to merge two molecules with different
> >>> symmetry?
> >>>
> >>> I will explain:
> >>>
> >>> I have done the molecular replacement using the domains of the
> >>> molecules separately, now I have to put all together, however
> >>> they have a different symmetry.
> >>>
> >>>
> >>> I will appreciate any kind of help.
> >>>
> >>>
> >>>
> >>> Regards,
> >>>
> >>>
> >>>
> >>>
> >>> Mnika
> >>>
> >>> -- __o _`\<,_ (*)/ (*) -+-+-+-+-+-+- * * * * * * * * * * * * *
> >>> * * * * * * * * * * * * * * * * * * * * * * * * * * ...E tudo
> >>> muda...
> >>>
> >>> * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
> >>> * * * * * * *
> >>>
> >>
> >>
> >>
> >
> >
>
> - --
> - --
> Dr Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
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...E tudo muda...
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