[ccp4bb] Post-doc - Structural biology CEA Saclay 30km of Paris

[OCHSENBEIN Françoise]

  Post-doc - Structural biology for the understanding of the mechanisms of 
assembly of chromatin coupled with homologous recombination

A postdoctoral position is available in the team of Molecular Assemblies and 
Integrity of Genomes of F. Ochsenbein (CEA SACLAY, CNRS I2BC, 
http://www.i2bc.paris-saclay.fr/spip.php?article1019=en). The laboratory 
is involved in studying histone assembly coupled to DNA replication and 
implications for genome integrity1-7. A project has been funded in 
collaboration with the group of S. Lambert 
(https://science.curie.fr/recherche/biologie-et-chimie-des-radiations-cellulaires-et-cancer/u934-umr3215-genetique-et-biologie-du-developpement/equipe-lambert/)
 and M. Amor-Gueret 
(https://science.curie.fr/recherche/biologie-et-chimie-des-radiations-cellulaires-et-cancer/u934-umr3215-genetique-et-biologie-du-developpement/equipe-amor-gueret/)
 for studying of the coupling between chromatin assembly mechanisms by histone 
chaperones and homologous recombination DNA repair8. Various methods in 
structural biology (NMR, x-ray crystallography, ITC, Chromatography, fork 
reconstitutions) combined with genetic approaches in fission yeast will be will 
be used to gain insights into mechanistic understanding of these processes and 
how dysfunctions of these pathways are involved in the development of cancers. 
The project will benefit from a large and rich scientific environment of the 
new university Paris-Saclay, the proximity of the synchrotron Soleil, and 
facility for biomolecular NMR of the CEA Saclay.
The candidate should have a PhD in the field of structural biology, NMR, and / 
or protein crystallography, with experience in biochemistry, production of 
recombinant proteins. Contract of 1 year renewable 2 times. Possibility of 
recruitment in permanent contract.

Position to be filled before the end of 2017.
Remuneration: according to salary scale and experience.
Send cover letter and CV by e-mail to: 
francoise.ochsenb...@cea.fr


(1) Mousson, F.; Lautrette, A.; Thuret, J. Y.; Agez, M.; Courbeyrette, R.; 
Amigues, B.; Becker, E.; Neumann, J. M.; Guerois, R.; Mann, C.; Ochsenbein, F., 
Proceedings of the National Academy of Sciences of the United States of America 
2005, 102 (17), 5975-80.

(2) Agez, M.; Chen, J.; Guerois, R.; van Heijenoort, C.; Thuret, J. Y.; Mann, 
C.; Ochsenbein, F., Structure 2007, 15 (2), 191-9.

(3) Galvani, A.; Courbeyrette, R.; Agez, M.; Ochsenbein, F.; Mann, C.; Thuret, 
J. Y., Molecular and cellular biology 2008, 28 (11), 3672-85.

(4) Jiao, Y.; Seeger, K.; Lautrette, A.; Gaubert, A.; Mousson, F.; Guerois, R.; 
Mann, C.; Ochsenbein, F., Proceedings of the National Academy of Sciences of 
the United States of America 2012, 109 (8), 2866-71.

(5) Abascal, F.; Corpet, A.; Gurard-Levin, Z. A.; Juan, D.; Ochsenbein, F.; 
Rico, D.; Valencia, A.; Almouzni, G., Molecular biology and evolution 2013, 30 
(8), 1853-66.

(6) Richet, N.; Liu, D.; Legrand, P.; Velours, C.; Corpet, A.; Gaubert, A.; 
Bakail, M.; Moal-Raisin, G.; Guerois, R.; Compper, C.; Besle, A.; Guichard, B.; 
Almouzni, G.; Ochsenbein, F., Nucleic acids research 2015, 43 (3), 1905-17.

(7) Sauer, P. V.; Timm, J.; Liu, D.; Sitbon, D.; Boeri-Erba, E.; Velours, C.; 
Mucke, N.; Langowski, J.; Ochsenbein, F.; Almouzni, G.; Panne, D., eLife 2017, 
6.

(8) Pietrobon, V.; Freon, K.; Hardy, J.; Costes, A.; Iraqui, I.; Ochsenbein, 
F.; Lambert, S. A., PLoS biology 2014, 12 (10), e1001968.

[ccp4bb] Post-doctoral position in Integrative Structural Biology in Paris, France

[OCHSENBEIN Françoise 148691]

Dear CCP4bb members,


A 2-year post-doctoral position is available in the Molecular Assemblies and 
Signaling team (http://www-dsv.cea.fr/en/ibitecs/6) at the CEA of Saclay, Gif 
sur Yvette (30 km south of Paris), France
Start date : The position is available from september 2014 and will be held 
open until January 2015.
Salary Range: 36000€ - 41000€ per annum
Candidate : The post-doc candidate should have a strong background in 
structural biology. Previous work in peptide chemistry, biochemistry or 
cellular biology will be appreciated.
Project : The project focuses on structural biology of chromatin associated 
factors. The post-doctoral fellow will undertake structural studies of proteins 
and complexes involved in the assembly of histones with DNA. He/She will 
combine complementary methods including NMR, Xray crystallography and 
biophysical techniques protein-protein interactions. These studies will be 
associated with functional studies in collaboration with the group of G. 
Almouzni (Institut Curie). He/She will also contribute to the design inhibitors 
of the interactions found pertinent in cancer research. This work will be 
carried out in tight collaboration with the bioinformaticians of the team and 
groups of chemists.
Selected publications :

-  Abascal, F., Corpet, A., Gurard-Levin, Z.A., Juan, D., Ochsenbein, 
F., Rico, D., Valencia, A., and Almouzni, G. (2013). Subfunctionalization via 
Adaptive Evolution Influenced by Genomic Context: The Case of Histone 
Chaperones ASF1a and ASF1b. Mol Biol Evol.

-  Jiao, Y., Seeger, K., Lautrette, A., Gaubert, A., Mousson, F., 
Guerois, R., Mann, C., and Ochsenbein, F. (2012). Surprising complexity of the 
Asf1 histone chaperone-Rad53 kinase interaction. Proc Natl Acad Sci U S A 109, 
2866-2871.

-  Barrault, M.B., Richet, N., Godard, C., Murciano, B., Le Tallec, B., 
Rousseau, E., Legrand, P., Charbonnier, J.B., Le Du, M.H., Guerois, R., et al. 
(2012). Dual functions of the Hsm3 protein in chaperoning and scaffolding 
regulatory particle subunits during the proteasome assembly. Proc Natl Acad Sci 
U S A 109, E1001-1010.

-  Mousseau, G., Raffy, Q., Thomas, O.P., Agez, M., Thai, R., Renault, 
J.P., Pin, S., Ochsenbein, F., Cintrat, J.C., and Rousseau, B. (2010). 
Footprinting of protein interactions by tritium labeling. Biochemistry 49, 
4297-4299.

-  Galvani, A., Courbeyrette, R., Agez, M., Ochsenbein, F., Mann, C., 
and Thuret, J.Y. (2008). In vivo study of the nucleosome assembly functions of 
ASF1 histone chaperones in human cells. Mol Cell Biol 28, 3672-3685.

-  Agez, M., Chen, J., Guerois, R., van Heijenoort, C., Thuret, J.Y., 
Mann, C., and Ochsenbein, F. (2007). Structure of the histone chaperone Asf1 
bound to the histone H3 C-terminal helix and functional insights. Structure 15, 
191-199.

-  Mousson, F., Ochsenbein, F., and Mann, C. (2007). The histone 
chaperone Asf1 at the crossroads of chromatin and DNA checkpoint pathways. 
Chromosoma 116, 79-93.

-  Mousson, F., Lautrette, A., Thuret, J.Y., Agez, M., Courbeyrette, 
R., Amigues, B., Becker, E., Neumann, J.M., Guerois, R., Mann, C., et al. 
(2005). Structural basis for the interaction of Asf1 with histone H3 and its 
functional implications. Proc Natl Acad Sci U S A 102, 5975-5980.

-

Send email applications (cover letter, CV and addresses of two referees) to :
Francoise Ochsenbein
francoise.ochsenb...@cea.frmailto:guerois%40cea.fr
iBiTec-S - Bat 144 p27 - Pc 22 – CEA
91191 Gif sur Yvette cedex – France
tel : +33 (0)1 69 08 96 79/fax : +33 (0)1 69 08 47 12





---
Francoise Ochsenbein
CEA Saclay
IBITEC-S/SB2SM/LBSR
Point courrier 22, Batiment 144
91191 Gif sur Yvette cedex
tel 00 33 1 69 08 96 79
fax 00 33 1 69 08 47 12
mail francoise.ochsenb...@cea.frmailto:francoise.ochsenb...@cea.fr
http://www-dsv.cea.fr/ibitecs/6

[ccp4bb] EMBO Workshop Advances in protein-protein analysis and modulation

[OCHSENBEIN Françoise 148691]

Dear all,

I would like to announce this EMBO work-shop dedicated to protein-protein 
interactions with a large foccus on structural biology.

“Advances in protein-protein analysis and modulation”, 9-12 september 2014 in 
Hyères, France

Application deadline: March 31 2014

Further information about how to apply, grants and fees can be found below and 
at http://events.embo.org/14-ppi/index.html

Organizing comity : Xavier Morelli (FR), Francoise Ochsenbein (FR), Andrew 
Wilson (UK), Chandra Verma (SG), Stephane Betzi (FR)

Confirmed speakers : Tom Blundell (UK); Cristina Cardoso (DE); David Fairlie 
(AU); Pierre Colas (FR) ; Christian Heinis (CH); Philippe Minard (FR); Michael 
Nilges (FR) Franca Fratarnali (UK); Ora Schueler-Furman (IL); Gianni 
Defabritiis (ES) Stefan Knapp (UK); Giovanna Zinzalla (SE) ; Nicolas Wolff (FR) 
; Christian Ottmann (NL); Michele Arkin (USA) Anna Mapp (USA); Bruno 
Villoutreix (FR)

Location : Presqu'ile de Giens. The EMBO Workshop will be hosted at the Club 
Belambra – Hyères, located in a secluded peninsula of the Mediterranean coast, 
on the French Riviera (south of France).


Protein-Protein interactions (PPIs) play central role in the regulation of 
biological processes. After more than a decade spent struggling to discover 
what features of a protein are ‘druggable’, the modulation of protein–protein 
interactions (PPIs) has emerged from both academic and corporate research as a 
promising therapeutic strategy to treat human disease. As such, PPI modulators 
(PPIMs) are the next generation of highly innovative drugs that will reach the 
market in the next decade. The challenge now is to develop techniques, 
chemistry and knowhow required to rationally identify new targets and design 
potential PPI modifying compounds. This is very different from generating small 
molecules that target the catalytic sites of enzymes and will require different 
approaches in terms of chemistry, screening assays, in silico modeling and 
cellular techniques, not to mention the need of basic understanding of PPI 
biophysics. The EMBO Workshop aims to bring together experts from various 
fields who work on understanding and modulating PPIs and also to create a 
competitive European network.
Topics:
§  PPI: New Targets / In Cells
§  PPI: Technological Advances / Methods
§  PPI analysis: Docking/Energetics/Prediction
§  PPPI modulation: Successful Examples
§  PPPI modulation: Chemical Biology / Chemoinformatics