[ccp4bb] Protein fold and the moonlighting function
Dear All I am curious to know about the promiscuous activity of a protein based on their fold. Can a protein having same fold also have same function (say not 100% but some activity) as the homologous structure. Please also let me know few relevant PDB structures where such kind of activity is shown promising. Regards Rajnandani Kashyap PhD Student To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
[ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?
Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price? Thanks in advance for sparing your valuable time and efforts. Regards Rajnandani Kashyap India To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
Re: [ccp4bb] Protein Purification- reg
Dear Amala Please increase NaCl concentration to 200mM from 50mM. That can help you out by increasing affinity of your protein to bead and will delay the elution time. On Mon, Aug 13, 2018, 7:20 PM amala mathimaran wrote: > Dear All > > I am working with HIS – tag protein in N-terminal (hexa histidine). The > protein from Prokaryotic origin cloned into pET30a+ vector and expressed in > *E.coli > *BL21 cells. The expression was good. I am trying to purify a protein > using Ni-affinity column equilibrate with Buffer A 50mM Tris pH7.5, 50mM > NaCl, 15mM imidazole, 3mM BME, 5%glycerol and eluted with Buffer B ( same > as buffer A but 250mM imidazole). I eluted the protein in step wise > gradiant. the protein was less binded in Ni affinity IMAC column because > eluted fraction contain less amount and the protein remain present in the > Flow through. Can any one suggest how to increase the binding affinity of > the protein and how to purify the protein. The protein PI was 6.33 > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
