[ccp4bb] NIST/NRC Postdoctoral Position in Structural Biophysics
NIST/NRC Postdoctoral Position Available in Structural Biophysics in Maryland, USA A postdoctoral position is available at the Institute for Bioscience Biotechnology Research located in Rockville, Maryland, USA (http://www.ibbr.umd.edu/). This position will investigate how N-linked glycosylation of the interleukin-7 receptor affects its binding to interleukin-7 relative to the unglycosylated receptor. The enhanced binding affinity of the glycosylated receptor to interleukin-7 is allosteric in nature, that is, the N-glycans do not participate directly in the binding interface. A biophysical approach using surface plasmon resonance, circular dichroism, fluorescence, and calorimerty will be employed. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, protein expression and purification. Prior experience in biophysical techniques and insect cell culture is preferred. NIST/NRC postdoctoral positions must be US citizens and more information can be found at http://www.nist.gov/iaao/postdoc.cfm. For further information and interest please contact Dr. Scott Walsh (email: swals...@umd.edu or phone: (240) 314-6478).
[ccp4bb] Postdoctoral Position Available in Structural Biophysics and Protein Engineering in Rockville, Maryland, USA
A NIH funded postdoctoral position is available at the Institute for Bioscience Biotechnology Research (IBBR) at the University of Maryland in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the structural, biochemical, and biophysical levels. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, bacterial/insect cell protein expression and purification. Prior experience in structural (X-ray crystallography or NMR spectroscopy) and biophysical techniques is preferred. Salary will be commensurate upon experience. For further information (http://www.ibbr.umd.edu/profiles/scott-walsh) or to apply for the position please send a cover letter, CV, and two references to Dr. Scott Walsh (email: swals...@umd.edu).
[ccp4bb] Postdoctoral Position Available in Structural Biophysics and Protein Engineering in Rockville, Maryland, USA
Postdoctoral Position Available in Structural Biophysics and Protein Engineering in Rockville, Maryland, USA A NIH funded postdoctoral position is available for a highly motivated individual at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the structural and biophysical levels. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, bacterial and insect cell protein expression and purification. Prior experience in structural (X-ray crystallography or NMR spectroscopy) and biophysical techniques (circular dichroism, calorimetry, fluorescence, etc.) is preferred. Salary will be commensurate upon experience. For further information or to apply for the positions please send a cover letter, CV, and two references to Dr. Scott Walsh (email: wal...@umbi.umd.edu).
[ccp4bb] UV microscope for screening
Dear CCP4BB members, I am shopping around for a UV microscope for the lab and would like to get some feedback about peoples' experiences using UV microscopes for screening crystals. Best, Scott * Scott T. R. Walsh, Ph.D. Assistant Professor Center for Advanced Research in Biotechnology University of Maryland Biotechnology Institute Rm 3127E CARB II 9600 Gudelsky Drive Rockville, MD 20850 email: wal...@umbi.umd.edu phone: (240) 314-6478 fax: (240) 314-6255-
[ccp4bb] Postdoctoral Positions Available in Structural Biophysics and Protein Engineering in Rockville, Maryland, USA
Postdoctoral Positions Available in Structural Biophysics and Protein Engineering in Rockville, Maryland, USA Two NIH funded postdoctoral position are available for highly motivated individuals at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the structural, biochemical, and biophysical levels. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, bacterial and insect cell protein expression and purification. Prior experience in structural (X-ray crystallography or NMR spectroscopy) and biophysical techniques is preferred. Salary will be commensurate upon experience. For further information or to apply for the positions please send a cover letter, CV, and two references to Dr. Scott Walsh (email: wal...@umbi.umd.edu).
Re: [ccp4bb] write out unmerged Scalepack file in HKL2000
Hi Alexandra, Sure. Go to the Macros tab and add no merge original index during scaling window. Cheers, Scott * Scott T. R. Walsh, Ph.D. Assistant Professor Center for Advanced Research in Biotechnology University of Maryland Biotechnology Institute Rm 3127E CARB II 9600 Gudelsky Drive Rockville, MD 20850 email: wal...@umbi.umd.edu phone: (240) 314-6478 fax: (240) 314-6255 -- - Original Message - From: Alexandra Deaconescu deac...@brandeis.edu Date: Wednesday, September 9, 2009 8:50 pm Subject: [ccp4bb] write out unmerged Scalepack file in HKL2000 To: CCP4BB@JISCMAIL.AC.UK Hi all: Is there a way to write out UNMERGED Scalepack files from HKL2000 ? In the older days of Scalepack, I remember you could have a NO MERGE in the script, but what about the newer HKL2000? If there is a way , well, I could not find it... Thanks a lot, Alex Alexandra M. Deaconescu Postdoctoral Fellow Grigorieff Laboratory Brandeis University Rosenstiel Center MS 029 415 South St. Waltham, MA 02454 USA For deliveries: Brandeis University Kalman Dock 415 South St. Waltham, MA 02454 USA
[ccp4bb] SOMoRe
Dear CC4BB users, Does any one know how to get SOMoRe for molecular replacement? The old link and email to Diane Jamrog no longer functions. Also, does any one have a Mac Intel version? Best regards, Scott * Scott T. R. Walsh, Ph.D. Center for Advanced Research in Biotechnology University of Maryland Biotechnology Institute Rm 3127E CARB II 9600 Gudelsky Drive Rockville, MD 20850 email: wal...@umbi.umd.edu phone: (240) 314-6478 fax: (240) 314-6255 --
Re: [ccp4bb] low UV reading on AKTA prime
Hi Matt, Check to make sure the A280 filter is clean. Ours was filthy. You might need to replace this (~$350). We were experiencing the same thing you mentioned and just had the GE technician out today. Also, make sure the A280 dial is set correctly on the lamp. Please check the manual for guidance. Best, Scott - Original Message - From: Matt Colins matt.colins2...@yahoo.com Date: Wednesday, July 1, 2009 4:40 pm Subject: [ccp4bb] low UV reading on AKTA prime To: CCP4BB@JISCMAIL.AC.UK Hi all, We recently got low UV reading on our AKTA prime. We contacted GE healthcare, but was told that the UV reading on AKTA prime should be 20% of the spectrometer reading, because the path length of the flow cell of AKTA prime is only 2 mm (20% of the pathlength of a spectrometer cuvette). I am not sure whether that is the case, but we used to get much higher UV readings on the same AKTA prime (almost the same as the spectrometer reading). The UV reading just keeps dropping over time in the past several months. Here I have two questions. First, should the UV reading on AKTA prime be 20% of the spectrometer reading? Second, what could go wrong with our AKTA prime? (I know it is not the lamp, because we put in a new lamp and it didn't solve the problem) Thanks a lot! Matt
[ccp4bb] Postdoctoral Position Available in Structural Biology/Biophysics in Maryland, USA
A NIH funded postdoctoral position is available at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the structural, biochemical, and biophysical levels. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, bacterial/insect cell protein expression and purification. Prior experience in structural (X- ray crystallography or NMR spectroscopy) and biophysical techniques is preferred. Salary will be commensurate upon experience. For further information or to apply for the position please send a cover letter, CV, and two references to Dr. Scott Walsh (email: wal...@umbi.umd.edu).
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[ccp4bb] Postdoctoral Positions Available in Structural Biophysics in Maryland, USA
Postdoctoral Positions Available in Structural Biophysics in Maryland, USA Several NIH funded postdoctoral positions are available at the Center for the Advanced Research in Biotechnology (CARB) at the University of Maryland Biotechnology Institute in Rockville, Maryland, USA. Several research projects are available to study the interleukin-7 signaling pathway at the structural, biochemical, and biophysical levels. Candidates must have a Ph.D. degree in biochemistry or a related field, with experience in molecular biology, protein expression and purification. Prior experience in structural (X-ray crystallography or NMR spectroscopy) and biophysical techniques is preferred. Salary will be commensurate upon experience. For further information or to apply for the positions please send a cover letter, CV, and two references to Dr. Scott Walsh (email: [EMAIL PROTECTED]).
Re: [ccp4bb] Reminder - still no 3D stereo under OS X 10.5
I think it is only X11 apps. I am able to use 3D stereo using MacPyMOL on my Mac tower runing 10.5.2. Cheers, Scott Is this true for native aqua gui programs, or just the X11 ones? On Thu, June 26, 2008 1:50 pm, eortlund wrote: Hi Everyone, I am writing everyone just to remind all those who are thinking of upgrading to 10.5 that currently quad-buffered stereo will not work for coot, pymol etc... since Apple has not done a good job with the video drivers in Leopard. So if you would like to use 3D stereo viewing, do not purchase any new Mac hardware since it will not run OS X 10.4. Eric -- Eric Ortlund, Ph.D. Assistant Professor Department of Biochemistry Emory University School of Medicine 1510 Clifton Road, NE, Room G235 Atlanta, GA 30322 Tel 404-727-5014 Fax 404-727-2738 [EMAIL PROTECTED] William G. Scott Contact info: http://chemistry.ucsc.edu/~wgscott/ *** Scott T. R. Walsh, Ph.D. Assistant Professor Department of Molecular and Cellular Biochemistry The Ohio State University College of Medicine 467 Hamilton Hall (Office) 479/483 Hamilton Hall (Lab) 1645 Neil Avenue Columbus, OH 43210 E-Mail: [EMAIL PROTECTED] Office Phone: 614-688-5802 Lab Phone: 614-688-8630 Fax: 614-292-4118 --
