Re: [ccp4bb] Butandiol as cryopotectant

[Steve Tuske]

20 percent (v/v).

Steve

> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delivered via an Android.
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> On Mar 22, 2010 11:45 PM, "Steve Tuske"  wrote:
>
> Hi Ulrike,
>
> 2,3-butanediol has worked very nicely as a cryoprotectant.  In my hands it
> mostly seems to decrease mosaicity.  I was able to switch from a
> three-step soaking protocol with glycerol and glucose to a quick (30
> second to one minute) soak with 2,3-BD.
>
> It's a winner.
>
> Steve
>
> I
>
>> Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. >
>> >
> Tim > > On Mon, Mar 22...
>

Re: [ccp4bb] Butandiol as cryopotectant

[Steve Tuske]

Hi Ulrike,

2,3-butanediol has worked very nicely as a cryoprotectant.  In my hands it
mostly seems to decrease mosaicity.  I was able to switch from a
three-step soaking protocol with glycerol and glucose to a quick (30
second to one minute) soak with 2,3-BD.

It's a winner.

Steve

I

> Hello Ulrike,
>
> in our lab 2,3-Butanediol has had a good reputation.
>
> Tim
>
> On Mon, Mar 22, 2010 at 01:32:33PM +, Ulrike Demmer wrote:
>> Hi everybody,
>>
>> has anyone experience with Butandiol and its different isoforms as
>> cryoprotectant ?
>>
>> Thanks,
>>
>> Ulrike
>
> --
> --
> Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
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>
>