Re: [ccp4bb] Off-topic, protein in dye-front (ion front?) on native-PAGE

[zeyaul islam]

Try Tricine gel. It is particularly suited for low molecular wt proteins
and it will give you very good resolution. Even you can run it overnight at
30 V (16-18 hours).

On Thu, Jan 19, 2017 at 9:33 AM, Walt  wrote:

> Hi,
>
> I have a small protein (~9 kDa) with acidic pI (~4).
> When I run 18% native-PAGE, it appears my protein is in the dye front.
> How can I fix this problem? Changing the pH of separating gel
> might help? How about gradient native-PAGE? Thank you!
>
> Walt
>

Re: [ccp4bb] About protein aggregation～！

[zeyaul islam]

Hello Gaoyina
S. pombe riboflavin synthase has similar situation . It is homotrimeric in
solution state but the protein is monomeric in the crystal structure.

Gerhardt S, Schott AK, Kairies N, Cushman M, Illarionov B, Eisenreich W,
Bacher A, Huber R, Steinbacher S, Fischer M. Studies on the reaction
mechanism of
riboflavin synthase: X-ray crystal structure of a complex with
6-carboxyethyl-7-oxo-8-ribityllumazine. Structure. 2002 Oct;10(10):1371-81.
PubMed PMID: 12377123.


Thanks

Zeyaul


On Tue, May 26, 2015 at 11:56 PM, dpsk  wrote:

> Hi,
> Recentely,we determined a *monomer* protein structure which looks like an
> *dimer* in solution via size-exclusion chromatography and SAXS,
> have you guys met such a situation before? or some references,
>
> Thank you for your reading
>
> Best wishes,