Re: [ccp4bb] Bicelle Crystallization in a Gryphon Robot

2012-02-16 Thread Poul Nissen 
Sorry for self promotion but you might also consider the HILIDE method which is 
very similar to bicelle and sponge, only having fully solubilized 
protein:lipid:detergent complexes as input sample and therefore being fully 
compatible with regular liquid handling robotics.
See Gourdon P et al 2011, Crystal Growth & Design 11, 2098-2106 for the method, 
and Sonntag Y et al 2011, Nature Comm 2, 304 for a direct look at the bilayers 
formed in the crystal
Poul



On 16/02/2012, at 23.53, Cory Brooks  wrote:

> Hello all;
> 
> I am wondering if anyone has any experience getting a ARI Gryphon robot to
> pick up protein in a bicelle solution?
> The nano needle on our robot does not seem to want to suck the sample up
> in a variety of liquid class settings.
> Any suggestions out there on if this can be made to work?
> 
> Cheers,
> Cory
> 
> 
> Cory Brooks,Ph.D.
> 
> University of Alberta
> Postdoctoral Fellow

Re: [ccp4bb] Bicelle Crystallization in a Gryphon Robot

2012-02-16 Thread Pius Padayatti 
there is one nice JOVE video and article from Jeff Abramson lab
which was neat showing using mosquito to set up bicelle.
No offense to Art people

J Vis Exp. 2012 Jan 9;(59). pii: 3383. doi: 10.3791/3383.

High-throughput Crystallization of Membrane Proteins Using the Lipidic
Bicelle Method.

Ujwal R, Abramson J.

link video
http://www.jove.com/video/3383/high-throughput-crystallization-of-membrane-proteins-using-the-lipidic-bicelle-method

padayatti

On Thu, Feb 16, 2012 at 5:53 PM, Cory Brooks  wrote:
> Hello all;
>
> I am wondering if anyone has any experience getting a ARI Gryphon robot to
> pick up protein in a bicelle solution?
> The nano needle on our robot does not seem to want to suck the sample up
> in a variety of liquid class settings.
> Any suggestions out there on if this can be made to work?
>
> Cheers,
> Cory
>
>
> Cory Brooks,Ph.D.
>
> University of Alberta
> Postdoctoral Fellow



-- 
Pius S Padayatti,PhD,
Phone: 216-658-4528

Re: [ccp4bb] Bicelle Crystallization in a Gryphon Robot

2012-02-16 Thread Art Robbins 
Hello Cory,



Unfortunately your Crystal Gryphon was not designed to work with all
bicelle solutions.

However, your Crystal Gryphon is easily upgradeable with our new LCP
attachment.

This will allow you to do LCP, Bicelle and Sponge Phase experiments.

If you have any further questions about the Gryphon please contact David

Terrell or Dave Wright at our office (888 658 5300).



Regards,

Art Robbins

On Thu, Feb 16, 2012 at 2:53 PM, Cory Brooks  wrote:

> Hello all;
>
> I am wondering if anyone has any experience getting a ARI Gryphon robot to
> pick up protein in a bicelle solution?
> The nano needle on our robot does not seem to want to suck the sample up
> in a variety of liquid class settings.
> Any suggestions out there on if this can be made to work?
>
> Cheers,
> Cory
>
>
> Cory Brooks,Ph.D.
>
> University of Alberta
> Postdoctoral Fellow
>

[ccp4bb] Bicelle Crystallization in a Gryphon Robot

2012-02-16 Thread Cory Brooks 
Hello all;

I am wondering if anyone has any experience getting a ARI Gryphon robot to
pick up protein in a bicelle solution?
The nano needle on our robot does not seem to want to suck the sample up
in a variety of liquid class settings.
Any suggestions out there on if this can be made to work?

Cheers,
Cory


Cory Brooks,Ph.D.

University of Alberta
Postdoctoral Fellow