Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.
You lose about 1 microlitre of water per well per day with polystyrene Linbro plates - it goes through the plastic Polypropylene and COC are better (or maybe worse if the evaporation encourages crystallization) -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en patr...@douglas.co.ukDouglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Diana Tomchick Sent: 15 January 2009 21:56 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film. Are you saying that you'd like to re-use the plate with the original screening solutions, or that you plan to clean the plates, then dispense fresh screening solutions? If you would like to re-use the original screening solutions, beware...after many weeks, they will not be the same concentrations (and in some cases, not even the same pH) as they were when first dispensed into the plates. Slow evaporation of water occurs through the plastic of the plates as well as through the tape. Diana On Jan 15, 2009, at 3:34 PM, Francis E Reyes wrote: Does such a thing exist? A 24-well microplate configuration where in substitution of glass cover slips, you have a roll of tape templated such that there are circular areas where you can add your protein where there is no adhesive, but there is adhesive everywhere else? This may be a nightmare for plate manufacturers, but to reuse the plate, you just throw away the film and tear a new one. Thanks! FR - Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D * * * * * * * * * * * * * * * * * * * * * * * * * * * * Diana R. Tomchick Associate Professor University of Texas Southwestern Medical Center Department of Biochemistry 5323 Harry Hines Blvd. Rm. ND10.214B Dallas, TX 75390-8816, U.S.A. Email: diana.tomch...@utsouthwestern.edu 214-645-6383 (phone) 214-645-6353 (fax)
[ccp4bb] Crystallography plates, hanging drop but templated sealing film.
Does such a thing exist? A 24-well microplate configuration where in substitution of glass cover slips, you have a roll of tape templated such that there are circular areas where you can add your protein where there is no adhesive, but there is adhesive everywhere else? This may be a nightmare for plate manufacturers, but to reuse the plate, you just throw away the film and tear a new one. Thanks! FR - Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.
This sounds very similar to a nifty little device the folks in Buffalo came up with: J. Appl. Cryst. (1992). 25, 324-325[ doi:10.1107/S0021889891011354 ] HANGMAN: a macromolecular hanging-drop vapor-diffusion technique J. R. Luft and G. T. DeTitta On 15 Jan 2009, at 4:34 PM, Francis E Reyes wrote: Does such a thing exist? A 24-well microplate configuration where in substitution of glass cover slips, you have a roll of tape templated such that there are circular areas where you can add your protein where there is no adhesive, but there is adhesive everywhere else? This may be a nightmare for plate manufacturers, but to reuse the plate, you just throw away the film and tear a new one. Thanks! FR - Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D --- Patrick J. Loll, Ph. D. Professor of Biochemistry Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 pat.l...@drexelmed.edu
Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.
Why not just use a 96 well plate. Should be able to do this by hand with a large enough drop. You can get them from either TTP Labtech or Grace Biosystems, I think that is the name. Cheers, Len Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval Norman, OK 73032 lmtho...@ou.edu Office: 405-325-1126 Lab: 405-325-7571 On Jan 15, 2009, at 3:34 PM, Francis E Reyes wrote: Does such a thing exist? A 24-well microplate configuration where in substitution of glass cover slips, you have a roll of tape templated such that there are circular areas where you can add your protein where there is no adhesive, but there is adhesive everywhere else? This may be a nightmare for plate manufacturers, but to reuse the plate, you just throw away the film and tear a new one. Thanks! FR - Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
