[ccp4bb] Dear ccp4
Dear CCp4 Thank you for all your and information suggestions regarding question Best Regards Rana
[ccp4bb] Dear ccp4
Dear ccp4 I am working on hbx (Hepatitis B x protein for both that duck and the human) I worked first on the dhbx which contained the vector pRSET-A and I didn’t get any results, then I changed the vector ( thanks to many of you who provided me with information, papers and books) using p-MAL c2x which worked perfectly throughout the whole procedure, I even now have about 60% soluble protein, then I started working on the hhbx,also changing the vector from pRSET-A to p-MAL c2xat first the PCR was successful with an obvious band on the gel, so I proceeded with double digestion using both restriction enzymes, incubated 3h then applied the insert on the gel, surprisingly what I observed was a slight band on the gel, after purifying the insert I checked the concentration which decreased from 35ng/ul before purification to 5.2ng/ul after purification, I would be grateful if any one has any idea or suggestions regarding this situation Best Regards Rana
[ccp4bb] dear ccp4
Dear ccp4 I am working on hbx (Hepatitis B x protein for both that duck and the human) I worked first on the dhbx which contained the vector pRSET-A and I didn’t get any results, then I changed the vector ( thanks to many of you who provided me with information, papers and books) using p-MAL c2x which worked perfectly throughout the whole procedure, I even now have about 60% soluble protein, then I started working on the hhbx,also changing the vector from pRSET-A to p-MAL c2xat first the PCR was successful with an obvious band on the gel, so I proceeded with double digestion using both restriction enzymes which are BamH1 and Hind111, incubated 3h then applied the insert on the gel, surprisingly what I observed was a slight band on the gel, after purifying the insert I checked the concentration which decreased from 35ng/ul before purification to 5.2ng/ul after purification, I would be grateful if any one has any idea or suggestions regarding this situation Best Regards Rana
