[ccp4bb] AW: [ccp4bb] Detergent/lipid crystal diffraction pattern?
Hi Pius DS_1.png - protein diffraction ! DSA6E.png - surface ice formation and protein crystal which did not survive the freezing !! DSA2F.png - protein crystal which does not diffract, but cryo condition is right ! Psp4f.png - internal ice formation plus some surface ice also protein crystal Cheers Stefan
Re: [ccp4bb] Detergent/lipid crystal diffraction pattern?
Pius, Are you sure that you determined the correct cell. Which program did you use? Usually there are much less spots on an image when a crystal has so small unit size dimensions. To me the first crystal looks like protein. Send it to a synchrotron and process the data in XDS. They can process for you. Maia On 21/03/2011 4:33 PM, Maia Cherney wrote: Hi PS What is the unit cell dimensions in the first crystal? It looks like protein to me. Maia On 21/03/2011 2:03 PM, Pius Padayatti wrote: Hi all, We recently observed some diffraction from membrane protein crystallization drops diffraction that look like non-proteinaceous (please see attached files, from 4 different crystals grown in different conditions). Rains' question about about lipid and detergent diffraction is so relevant. This is most likely what lipids and detergent diffraction looks like? People with similar experience and know what could be these patterns might be from may have better suggestions and would like to hear all comments. first four images are from drops where detergent is DDM and and vapor diffusion while last image is from a crystal grown in mesophase (with monoolein). Padayatti PS On Sat, Mar 19, 2011 at 7:19 PM,Rain Field rainfiel...@163.com wrote: Hi All, I am wondering if the detergent or lipid crystal can have diffraction at low resolution. If they can, what does the diffraction pattern looks like? Are there any literatures describing these? Many thanks!
Re: [ccp4bb] Detergent/lipid crystal diffraction pattern?
Working with the H+-ATPase AHA2 (C12E8/DDM/Cymal-5 detergent mixed micelle) we always saw a ring at ~40Å which we attributed to the micelle, but we have no experimental evidence that it was indeed caused by detergents. Heres a animated gif of this phenomenon: http://www.bioxray.au.dk/~bjopp/pictures/zoom.gif Also recently I tested some very nice looking and surprisingly hexagonal xtal-like structures in a drop full of DDM crystals. I got this '6-fold' pattern: http://www.bioxray.au.dk/~bjopp/pictures/ddm_diff.png Again I'm guessing detergent, but I would like to hear any alternative explanations. :) hth -Bjørn -- Bjørn Panyella Pedersen, PhD Postdoc Macromolecular Structure Group Dept. of Biochemistry and Biophysics University of California, San Francisco MC2240, 600 - 16th Street S414 San Francisco, CA 94158-2517 Phone: +1 415-476-3937 E-mail: bj...@msg.ucsf.edu http://www.msg.ucsf.edu On 2011-03-19 16:19, Rain Field wrote: Hi All, I am wondering if the detergent or lipid crystal can have diffraction at low resolution. If they can, what does the diffraction pattern looks like? Are there any literatures describing these? Many thanks!
Re: [ccp4bb] Detergent/lipid crystal diffraction pattern?
Hi PS What is the unit cell dimensions in the first crystal? It looks like protein to me. Maia On 21/03/2011 2:03 PM, Pius Padayatti wrote: Hi all, We recently observed some diffraction from membrane protein crystallization drops diffraction that look like non-proteinaceous (please see attached files, from 4 different crystals grown in different conditions). Rains' question about about lipid and detergent diffraction is so relevant. This is most likely what lipids and detergent diffraction looks like? People with similar experience and know what could be these patterns might be from may have better suggestions and would like to hear all comments. first four images are from drops where detergent is DDM and and vapor diffusion while last image is from a crystal grown in mesophase (with monoolein). Padayatti PS On Sat, Mar 19, 2011 at 7:19 PM,Rain Field rainfiel...@163.com wrote: Hi All, I am wondering if the detergent or lipid crystal can have diffraction at low resolution. If they can, what does the diffraction pattern looks like? Are there any literatures describing these? Many thanks!
[ccp4bb] Detergent/lipid crystal diffraction pattern?
Hi All, I am wondering if the detergent or lipid crystal can have diffraction at low resolution. If they can, what does the diffraction pattern looks like? Are there any literatures describing these? Many thanks!
