Many thanks to all who took out some time to reply. Summary:
you can also use https://www.neb.com/en/products/c3037-nebexpress-iq-competent-e-coli-high-efficiency this has LacIq, we use it with all our pQE bassed vectors. Gentauer may still sell those… https://maxanim.com/strains/m15-prep4-escherichia-coli-strains/ we have just today obtained a plate with M15(pREP4) from someone at another lab in Madrid who also subscribes to ccp4bb. other people also offered the pREF4 plasmid Mark van Raaij Dpto de Estructura de Macromoleculas, lab 20B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092) > > >> Am 31.05.2024 um 13:17 schrieb Mark J. van Raaij <mjvanra...@cnb.csic.es>: >> >> Dear All. >> >> we are looking for E. coli strain M15 or SG13009 to use for expressing a >> protein from the vector pQE30. Are these strains still availabe commercially? >> If not, we'd be open for another way to obtain an aliquot. >> >> Wbw, >> >> Mark >> >> >> Mark van Raaij >> Dpto de Estructura de Macromoleculas, lab 20B >> Centro Nacional de Biotecnologia - CSIC >> calle Darwin 3 >> E-28049 Madrid, Spain >> tel. +34 91 585 4616 (internal 432092) >> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
