Re: [ccp4bb] How to rotate subunits

[Avinash Punekar]

Hi,

There is a tool InterfaceAnalyzer in the Rosetta software suite that can 
probably do this. More details here 
(https://www.rosettacommons.org/docs/latest/application_documentation/analysis/interface-analyzer)

Best wishes,
Avinash

Re: [ccp4bb] How to rotate subunits

[Eleanor Dodson]

Hmm - that is a bit vague..
There is a CCP4 program pdbset

pdbset xyzin monomer.pdb
rotate polar theta phi kappa
shift x y z
But you need to know what to rotate and shift..

Easier is to find a model and fit your monomers over the model.

Once you have the rotation done - pISA will tell you the buried surface
area.
Eleanor



On 22 September 2017 at 21:52, Vands  wrote:

> HI all,
>I have a Structure of dimer where I would like to rotate
> one monomer with respect to other to know the interface surface area
> buried in different orientations.
> which software should I use to generate different orientation?
> thanks for the help.
>
>
> --
> Vandna Kukshal
> Postdoctral Research Associate
> Dept. Biochemistry and Molecular Biophysics
> Washington University School of Medicine
> 660 S. Euclid
> , Campus
> Box 8231
> St. Louis, MO 63110
>

[ccp4bb] How to rotate subunits

[Vands]

HI all,
   I have a Structure of dimer where I would like to rotate one
monomer with respect to other to know the interface surface area buried in
different orientations.
which software should I use to generate different orientation?
thanks for the help.


-- 
Vandna Kukshal
Postdoctral Research Associate
Dept. Biochemistry and Molecular Biophysics
Washington University School of Medicine
660 S. Euclid, Campus Box 8231
St. Louis, MO 63110