Hard to comment without more information, but check how many molecules you
expect per asymmetric unit. If more than one look at the self rotation
function - it might help.
And you can with difficulty do a cross rotation between two data sets,
which sometimes suggests how crystal 1 is related to crystal 2.
But if you have the Se sites for both crystals, it is probably easier to
try to match them and get transformation matrices to use for
cross-averaging that way.
Is that your case?
Eleanor.
On 12 July 2013 03:33, Yuan SHANG shangyuan5...@gmail.com wrote:
Dear all,
Currently, I was stuck in a coiled-coil crystal. I have two
Se-derivative crystals in similar crystallization conditions. And the cell
parameters of these two are list below :
Crystal A: P21, a=69,b=43,c=135, beta=100.7
Crystal B: P21, a=29,b=230,c=42, beta=92.2
Assuming the crystal packings in A and B are overall similar, there
seems to be another P21 axis in crystal A, which may help to determine
the NCS parameters during phasing as there are two moleculars in the ASU.
Could anyone help to tell me how to derive the NCS parameters?
Best regards,
Yuan SHANG
