Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

[Jurgen Bosch]

Have you tried Guanidinium chloride?

Jürgen 

> On Jul 3, 2024, at 1:22 PM, Daniele de Sanctis  wrote:
> 
> Hi Flavio
> 
> I’d suggest to contact Regenfix 
> https://www.regenfix.eu/
> 
> Usually they give you back a column better than the original one completed 
> with an experimental calibration curve.
> 
> Cheers 
> 
> Daniele
> 
> 
> 
> On Wed 3 Jul 2024 at 18:56, Nicholas Clark 
>  > wrote:
>> Hi Flavio,
>> 
>> I’d recommend reaching out to Cytiva directly 
>> (scientificsupport...@cytiva.com ). 
>> We had an issue with oxidized metal on our S200 and they were able to 
>> provide useful suggestions (plus side is, you know what they suggest won’t 
>> ruin the resin).
>> 
>> Best,
>> 
>> Nick Clark
>> 
>> Nicholas D. Clark, PhD (He/Him)
>> University at Buffalo
>> Department of Structural Biology
>> Jacob's School of Medicine & Biomedical Sciences
>> 955 Main Street 
>> 
>> Buffalo, NY 14203 
>> 
>> 
>> 
>> On Wed, Jul 3, 2024 at 12:01 PM Flavio Di Pisa > > wrote:
>>> Dear community,
>>> do you have any advice on how to remove the fluorescein from a superdex 200 
>>> increase?
>>> Thank you
>>> Flavio.
>>> 
>>> To unsubscribe from the CCP4BB list, click the following link:
>>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 
>>> 
>> To unsubscribe from the CCP4BB list, click the following link:
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> 
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Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

[Daniele de Sanctis]

Hi Flavio

I’d suggest to contact Regenfix
https://www.regenfix.eu/

Usually they give you back a column better than the original one completed
with an experimental calibration curve.

Cheers

Daniele



On Wed 3 Jul 2024 at 18:56, Nicholas Clark <
b2b1c7e93c2d-dmarc-requ...@jiscmail.ac.uk> wrote:

> Hi Flavio,
>
> I’d recommend reaching out to Cytiva directly (
> scientificsupport...@cytiva.com). We had an issue with oxidized metal on
> our S200 and they were able to provide useful suggestions (plus side is,
> you know what they suggest won’t ruin the resin).
>
> Best,
>
> Nick Clark
>
> Nicholas D. Clark, PhD (He/Him)
> University at Buffalo
> Department of Structural Biology
> Jacob's School of Medicine & Biomedical Sciences
> 955 Main Street
> 
> Buffalo, NY 14203
> 
>
>
> On Wed, Jul 3, 2024 at 12:01 PM Flavio Di Pisa  wrote:
>
>> Dear community,
>> do you have any advice on how to remove the fluorescein from a superdex
>> 200 increase?
>> Thank you
>> Flavio.
>>
>> --
>>
>> To unsubscribe from the CCP4BB list, click the following link:
>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
>> 
>>
>
> --
>
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>



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Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

[Nicholas Clark]

Hi Flavio,

I’d recommend reaching out to Cytiva directly (
scientificsupport...@cytiva.com). We had an issue with oxidized metal on
our S200 and they were able to provide useful suggestions (plus side is,
you know what they suggest won’t ruin the resin).

Best,

Nick Clark

Nicholas D. Clark, PhD (He/Him)
University at Buffalo
Department of Structural Biology
Jacob's School of Medicine & Biomedical Sciences
955 Main Street
Buffalo, NY 14203


On Wed, Jul 3, 2024 at 12:01 PM Flavio Di Pisa  wrote:

> Dear community,
> do you have any advice on how to remove the fluorescein from a superdex
> 200 increase?
> Thank you
> Flavio.
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
> 
>



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