Hi Nikolas The first thing I would try is to solubilise the protein a bit more, maybe by adding a few % (1-5%) glycerol or ethylene glycol to the growth conditions when you set up the drops.
The next thing would be to experiment with ratio (protein:precipitant) and drop size. Lastly, trial addition of detergents to conditions. Best! Andy ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
