Dear all, I would just like to remind you of the upcoming deadline.
Best wishes Manfred, on behalf of the HZB-MX team the next MX-proposal application deadline: March 01, 2019 is approaching https://www.helmholtz-berlin.de/user/beamtime/proposals/index_en.html As usual, all proposals will be handled with the new version of GATE, GATE2.0, https://www.helmholtz-berlin.de/pubbin/hzbgate Hereby we would like to invite the submission of new proposals for MX-beamtime at the HZB-MX beamlines for the next beam time period (09/2019-02/2020). In order to apply for beamtime, please register at the HZB on-line access tool "GATE" (https://www.helmholtz-berlin.de/pubbin/hzbgate) and submit a new beam time application proposal. With the new GATE version, the proposal categories have been improved. We now expect from each research group only ONE proposal, which can contain up to 20 individual projects. HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2 and BL14.3. The three beamlines are equipped with state-of-the-art instrumentation and are currently the most productive MX-stations in Germany with over 300 PDB depositions annually. Beamtime is granted based on the reviewed proposals and on reports from previous research activities. Please make sure to include them if available. Experimental setup: BL14.1: - Photon energy range: 5.5-16 keV (wavelength: 0.77502.25 A) - Photon flux: 1.8x10¹¹ Phot/sec x 100 mA at sample position (0.1-1 sec exposure time per frame) - PILATUS 6M detector, 141 mm-680 mm max. distance from the sample - Microdiffractometer (MD2) with Mini-kappa goniometer - Automatic sample changer (CATS), 90 sample storage capacity (SPINE-Pin & EMBL sample magazine compatibility) - User defined beam shaping from 50 µm-100 µm diameter possible - In situ crystal-screening using 96-well plates - 32-core XEON-CPU server, with 10GB uplink to Pilatus 6M - Data collection control via MXCuBE2 - Common MX-software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - AMPTEK-XRF detector and XFEPLOT software available We are also offering the hard- and software environment for carrying out UV-RIP experiments at BL14.1. For further information, please visit: https://www.helmholtz-berlin.de/forschung/oe/np/gmx/ancillary-facilities/uvrip_en.html BL14.2: - Photon energy range: 5.5-16 keV (wavelength: 0.77502.25 A) - Photon flux: 1.6x10¹¹ Phot/sec x 100 mA at sample position (0.05-1 sec exposure time per frame) - PILATUS 3S-2M detector with 1000 µm Si sensor thickness, 85 mm - 800 mm distance from the sample (a special mode with 55 mm distance is also available upon request) - Nanodiffractometer with fast air-bearing axis and on-axis sample microscope - User defined beam shaping from 30 µm-150 µm diameter possible - Data collection control via MXCuBE2 - G-ROB sample changer for SPINE and UNIPUCK support - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Common MX-software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - AMPTEK-XRF detector and XFEPLOT software available - UV-Microsprectrophotometer offline setup available If you need atomic resolution or better, BL14.2 is the beamline of choice for you. BL14.3: - Fixed photon energy: 13.8 keV (wavelength: 0.89 A) - Photon flux: 1.6x10exp10 Phot/sec x 100mA at sample position (3-20 sec exposure time per frame) - Rayonix MX-225 X-ray detector, 54 mm-450 mm distance from the sample - MD2S microdiffractometer with mini-kappa goniometer - In situ crystal-screening using 96-well plates - RT data collection - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Data collection control via MXCuBE2 - Common MX software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - REX rapid nozzle exchanger - HC-Lab dehydration device installed (please specify HC-Lab-beamtime in your proposal if needed) - AMPTEK-XRF detector and XFEPLOT software available Other facilities: - Ultra high performance stereo microscope Leica M205A, 20-255x zoom, 8 Mpixel CCD-camera - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) S1-biolab facilities (separate registration required): - Protein production and purification (AEKTA) - nL 96-well crystallization plate formulation and storage at 5°C and 20°C - Biophysical characterization with real time PCR (thermofluor assay) - Contactless compound pipetting using ATS The HZB-MX group is also providing expert assistance as well as access to a library of fragments for carrying out crystallographic fragment-screening experiments. For more information please see https://www.helmholtz-berlin.de/forschung/oe/np/gmx/fragment-screening/index_en.html or contact mswe...@helmholtz-berlin.de<mailto:mswe...@helmholtz-berlin.de>. Please visit our web page www.helmholtz-berlin.de/bessy-mx<http://www.helmholtz-berlin.de/bessy-mx> to obtain updated information about our experimental setup and other requirements. -- Dr. Manfred S. Weiss Macromolecular Crystallography Helmholtz-Zentrum Berlin Albert-Einstein-Str. 15 D-12489 Berlin Germany ________________________________ Helmholtz-Zentrum Berlin für Materialien und Energie GmbH Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V. Aufsichtsrat: Vorsitzender Dr. Karl Eugen Huthmacher, stv. Vorsitzende Dr. Jutta Koch-Unterseher Geschäftsführung: Prof. Dr. Bernd Rech (kommissarisch), Thomas Frederking Sitz Berlin, AG Charlottenburg, 89 HRB 5583 Postadresse: Hahn-Meitner-Platz 1 D-14109 Berlin ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
