[ccp4bb] Spam : Re: [ccp4bb] Protein Ligand interaction using SPR technique

[Debasish Kumar Ghosh]

Dear Praveen,

We have very good experience with protein ligand interaction with Biacore 3000 
using CM5 chip. The NTA chip is prone to nonspecific interactions and give 
erroneous results. However the gold standard is ITC since it is more sensitive 
than Biacore 3000. 
One quick note for using Biacore 3000 is to optimize the buffer. HBS appeared 
nice for us.
Hope that helps.

Cheers!!



Debasish

CSIR- Senior Research Fellow (PhD Scholar)
C/o: Dr. Akash Ranjan
Computational and Functional Genomics Group
Centre for DNA Fingerprinting and Diagnostics
Hyderabad, INDIA

Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com
Telephone: 0091-9088334375 (M), 0091-40-24749396 (Lab)
Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html



- Original Message -
From: Praveen Tripathi 
To: CCP4BB@JISCMAIL.AC.UK
Sent: Fri, 31 Mar 2017 10:31:43 +0530 (IST)
Subject: [ccp4bb] Protein Ligand interaction using SPR technique

Dear all,
Sorry for off-topic question.
I want to study protein interaction with few small molecules using SPR
(Machine- *BIACORE 3000*).

The recombinant protein expressed in bacterial expression system is of 92
kDa (with His tag), pI= 9.

*Question-*
1. What should be the chip of choice- *NTA chip or CM5 chip* of GE
healthcare?
2. Is there any alternative available for chips and machine?

Thanks in advance.

Regards

Praveen

[ccp4bb] Spam : Re: [ccp4bb] Protein Ligand interaction using SPR technique

[Debasish Kumar Ghosh]

Debasish Kumar Ghosh

CSIR- Senior Research Fellow (PhD Scholar)
C/o: Dr. Akash Ranjan
Computational and Functional Genomics Group
Centre for DNA Fingerprinting and Diagnostics
Hyderabad, INDIA

Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com
Telephone: 0091-9088334375 (M), 0091-40-24749396 (Lab)
Lab URL: http://www.cdfd.org.in/labpages/computational_functional_genomics.html



- Original Message -
From: Praveen Tripathi 
To: CCP4BB@JISCMAIL.AC.UK
Sent: Fri, 31 Mar 2017 10:31:43 +0530 (IST)
Subject: [ccp4bb] Protein Ligand interaction using SPR technique

Dear all,
Sorry for off-topic question.
I want to study protein interaction with few small molecules using SPR
(Machine- *BIACORE 3000*).

The recombinant protein expressed in bacterial expression system is of 92
kDa (with His tag), pI= 9.

*Question-*
1. What should be the chip of choice- *NTA chip or CM5 chip* of GE
healthcare?
2. Is there any alternative available for chips and machine?

Thanks in advance.

Regards

Praveen