Re: [ccp4bb] Temperature and crystallization

[Esko Oksanen]

  Dear Acoot,

  There is a paper by Monika Budayova-Spano 
(http://scripts.iucr.org/cgi-bin/paper?fw5105) on using temperature as a 
crystallisation variable that also has a number of useful references.

  HTH,
  Esko
_

Esko Oksanen, PhD
Instrument Scientist - Neutron Macromolecular Crystallography

European Spallation Source ESS AB
P.O Box 176, SE-221 00 Lund, Sweden
Visiting address: Tunavägen 24, Lund

Switchboard: +46 46 888 00 00
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E-mail: esko.oksa...@esss.se

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committed to the goal of Collectively building and operating the world's 
leading facility for research using neutrons by the second quarter of the 21st 
Century

On Nov 19, 2012, at 6:08 AM, Acoot Brett wrote:

Dear All,

Will you please give a comment on how the temperature influences on the 
possibility to get protein crystal, and how the temperatures used to get the 
protein crystals of the same protein influences the protein 3-D structures of 
the same protein got based on the crystals of the same protein got at different 
temperatures?

Cheers,

Acoot

Re: [ccp4bb] Temperature and crystallization

[Emmanuel Saridakis]

Dear Acoot,

In the website of the company Centeo, you can find the slides of a short 
presentation I had given three years ago on temperature and macromolecular 
crystalllisation. You may find some info there:

http://www.centeo.com/_fileupload/Temperature%20Control%20in%20PXTL.pdf

There is a lot more on the centeo.com site.

Best,

Emmanuel Saridakis

  - Original Message - 
  From: Acoot Brett 
  To: CCP4BB@JISCMAIL.AC.UK 
  Sent: Monday, November 19, 2012 7:08 AM
  Subject: [ccp4bb] Temperature and crystallization


Dear All,

Will you please give a comment on how the temperature influences on the 
possibility to get protein crystal, and how the temperatures used to get the 
protein crystals of the same protein influences the protein 3-D structures of 
the same protein got based on the crystals of the same protein got at different 
temperatures?

Cheers,

Acoot


   

[ccp4bb] Temperature and crystallization

[Acoot Brett]

Dear All,

Will you please give a comment on how the temperature influences on the 
possibility to get protein crystal, and how the temperatures used to get the 
protein crystals of the same protein influences the protein 3-D structures of 
the same protein got based on the crystals of the same protein got at different 
temperatures?

Cheers,

Acoot