[ccp4bb] about partial plroteolysis

2011-04-20 Thread xinghua qin 
hello CCp4ers:

Sorry about the off-topic question. My protein is a kinase which the
predicted kinase domain is from 32aa to 319aa.
But the protein sequence after partial proteolysis is from 52aa. what should
I do?  Are there any reasonable expanation?

Thanks in advance!

Xinghua Qin

-- 
Xinghua Qin
Room 4022, Center for Life Sciences,
China Agricultural University,
No.2, Yuan Ming Yuan West Road, Haidian District，
Beijing，China，100193
Tel: +86-10-62732672

Re: [ccp4bb] about partial plroteolysis

2011-04-20 Thread Ed Pozharski 
On Wed, 2011-04-20 at 14:27 +0800, xinghua qin wrote:
 But the protein sequence after partial proteolysis is from 52aa. what
 should I do?  Are there any reasonable expanation?

The protease cut your protein at position 52 sounds reasonable enough
to me

-- 
Hurry up before we all come back to our senses!
   Julian, King of Lemurs