Hi Faisal,
The solutions from BALBES have already gone through multiple rounds of
refinement which minimize the R/Rfree. If you make a number of drastic changes
to side chains or add waters, the R/Rfree should go down. However if the
structure fits the density well (with no side chains needing major adjustment)
and you have not yet added waters... you will not notice the R/Rfree go down.
What are you R/Rfree values?
---
Greg Costakes
PhD Candidate
Department of Structural Biology
Purdue University
Hockmeyer Hall, Room 320
240 S. Martin Jischke Drive, West Lafayette, IN 47907
** Hard work often pays of in time, but Procrastination always pays off now **
- Original Message -
From: "Faisal Tarique"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, August 7, 2012 4:40:38 PM
Subject: [ccp4bb] balbes solution
Dear all
i submitted one job in BALBES at YSBL server. The final outcome are showing the
result to be definite solution by stating it to be a 99%solution. but when i am
refining with refmac, Rwork and Rfree is not coming down despite my several
tries. In COOT i can see tye missing density for loop region. the data is of
2.5 angstrom resolution. --
Regards
Faisal
School of Life Sciences
JNU